                         SEQUENCE LISTING

<110>  E. I. du Pont de Nemours & Co., Inc.
     
 
<120>  MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS
        AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS

<130>  BB1598 US NA

<160>  198   

<170>  PatentIn version 3.4

<210>  1
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S consensus, optionally comprising M1, M2, M3, M8, 
       M12, M15, M16, M18, M21, M26, M38, M45, M46, M51, M63, M68, M69 
       and M70 mutation sites


<220>
<221>  misc_feature
<222>  (2)..(1270)
<223>  mutant delta-8 desaturase CDS

<400>  1
catggtgaag nnnnnncgac aggctctgcc cctcnnnatc gacggannnn nntacgacgt     60

ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact accagggacg    120

agatgctact gacgccttca tggttatgca ctctcaggaa nnnnnngaca agctcaagcg    180

aatgcccaag atcaacnnnn nntccgagct gcctccccag gctgccgtca acgaagctca    240

ggaggatttc cgaaagctcc gagaagagct gatcgccact ggcatgtttg acgcctctcc    300

cctctggtac tcgtacaaga tcnnnaccac cctgggtctt ggcgtgcttn nnnnnttcnn    360

nnnngtccag tacnnnnnnt acttcattgg tgctnnnnnn ctcggtatgc actaccagca    420

aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc gaaactggaa    480

taacnnnnnn ggtctggtct ttggcaacnn nnnncagggc ttctccgtga cctggtggaa    540

ggacagacac aacgcccatc attctgctac caacgttcag ggtcacgatc ccgacattga    600

taacctgcct ctgctcgcct ggtccgagga cgatgtcact cgagcttctc ccatctcccg    660

aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat    720

ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta    780

ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc tcaagnnnnn    840

nttccacctc ttctttatgc cctccatcct gacctcgnnn ctggtgttct ttgtttccga    900

gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg aaccactacc ctctggagaa    960

gatcggtgat tccgtctggg acggacatgg cttctctgtg ggtcagatcc atgagaccat   1020

gaacattcga cgaggcnnnn nnnnngactg gttctttgga ggcctgaact accagatcga   1080

gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga   1140

acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg aaggtctcgt   1200

catcctgctc cgatacctgn nnnnnttcgc tcgaatggcc gagaagcagc ccnnnnnnaa   1260

ggctnnntaa gc                                                       1272


<210>  2
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S consensus, optionally comprising M1, M2, M3, M8, 
       M12, M15, M16, M18, M21, M26, M38, M45, M46, M51, M63, M68, M69 
       and M70 mutation sites


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X = S (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X = K (synthetic codon-optimized) or S (mutant)

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  X = T (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  X = T (synthetic codon-optimized) or K (mutant)

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  X = T (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (54)..(54)
<223>  X = A (synthetic codon-optimized) or G (mutant)

<220>
<221>  MISC_FEATURE
<222>  (55)..(55)
<223>  X = F (synthetic codon-optimized) or Y (mutant)

<220>
<221>  MISC_FEATURE
<222>  (66)..(66)
<223>  X = P (synthetic codon-optimized) or Q (mutant)

<220>
<221>  MISC_FEATURE
<222>  (67)..(67)
<223>  X = S (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (108)..(108)
<223>  X = S (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (117)..(117)
<223>  X = G (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (118)..(118)
<223>  X = Y (synthetic codon-optimized) or F (mutant)

<220>
<221>  MISC_FEATURE
<222>  (120)..(120)
<223>  X = L (synthetic codon-optimized) or M (mutant)

<220>
<221>  MISC_FEATURE
<222>  (121)..(121)
<223>  X = M (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (125)..(125)
<223>  X = Q (synthetic codon-optimized) or H (mutant)

<220>
<221>  MISC_FEATURE
<222>  (126)..(126)
<223>  X = M (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (132)..(132)
<223>  X = V (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (133)..(133)
<223>  X = L (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (162)..(162)
<223>  X = L (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (163)..(163)
<223>  X = V (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (170)..(170)
<223>  X = G (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (171)..(171)
<223>  X = L (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (279)..(279)
<223>  X = T (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (280)..(280)
<223>  X = L (synthetic codon-optimized) or T (mutant)

<220>
<221>  MISC_FEATURE
<222>  (293)..(293)
<223>  X = L (synthetic codon-optimized) or M (mutant)

<220>
<221>  MISC_FEATURE
<222>  (346)..(346)
<223>  X = I (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (347)..(347)
<223>  X = I (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (348)..(348)
<223>  X = T (synthetic codon-optimized) or S (mutant)

<220>
<221>  MISC_FEATURE
<222>  (407)..(407)
<223>  X = A (synthetic codon-optimized) or S (mutant)

<220>
<221>  MISC_FEATURE
<222>  (408)..(408)
<223>  X = V (synthetic codon-optimized) or Q (mutant)

<220>
<221>  MISC_FEATURE
<222>  (418)..(418)
<223>  X = A (synthetic codon-optimized) or G (mutant)

<220>
<221>  MISC_FEATURE
<222>  (419)..(419)
<223>  X = G (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (422)..(422)
<223>  X = L (synthetic codon-optimized) or Q (mutant)

<400>  2

Met Val Lys Xaa Xaa Arg Gln Ala Leu Pro Leu Xaa Ile Asp Gly Xaa 
1               5                   10                  15      


Xaa Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Xaa Xaa Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Xaa Xaa Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Xaa Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Xaa Xaa Phe Xaa Xaa Val Gln Tyr Xaa Xaa Tyr Phe 
        115                 120                 125             


Ile Gly Ala Xaa Xaa Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Xaa Xaa Gly Leu Val Phe Gly Asn Xaa Xaa Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Xaa Xaa Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Xaa Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Xaa Xaa Xaa Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Xaa Xaa Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Xaa Xaa Lys Ala Xaa 
            420         


<210>  3
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S-23


<220>
<221>  misc_feature
<222>  (2)..(1270)
<223>  mutant EgD8S-23 delta-8 desaturase CDS

<400>  3
catggtgaag gcttctcgac aggctctgcc cctcgtcatc gacggaaagg tgtacgacgt     60

ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact accagggacg    120

agatgctact gacgccttca tggttatgca ctctcaggaa gccttcgaca agctcaagcg    180

aatgcccaag atcaaccagg cttccgagct gcctccccag gctgccgtca acgaagctca    240

ggaggatttc cgaaagctcc gagaagagct gatcgccact ggcatgtttg acgcctctcc    300

cctctggtac tcgtacaaga tcttgaccac cctgggtctt ggcgtgcttg ccttcttcat    360

gctggtccag taccacctgt acttcattgg tgctctcgtg ctcggtatgc actaccagca    420

aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc gaaactggaa    480

taacgtcctg ggtctggtct ttggcaacgg actccagggc ttctccgtga cctggtggaa    540

ggacagacac aacgcccatc attctgctac caacgttcag ggtcacgatc ccgacattga    600

taacctgcct ctgctcgcct ggtccgagga cgatgtcact cgagcttctc ccatctcccg    660

aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat    720

ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta    780

ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc tcaagaccct    840

gttccacctc ttctttatgc cctccatcct gacctcgatg ctggtgttct ttgtttccga    900

gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg aaccactacc ctctggagaa    960

gatcggtgat tccgtctggg acggacatgg cttctctgtg ggtcagatcc atgagaccat   1020

gaacattcga cgaggcatca ttactgactg gttctttgga ggcctgaact accagatcga   1080

gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga   1140

acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg aaggtctcgt   1200

catcctgctc cgatacctgt cccagttcgc tcgaatggcc gagaagcagc ccggtgccaa   1260

ggctcagtaa gc                                                       1272


<210>  4
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S-23, comprising M1, M2, M3, M8, M12, M15, M16, M18, 
       M21, M26, M45, M46, M68 and M70 mutation sites

<400>  4

Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys 
1               5                   10                  15      


Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe 
        115                 120                 125             


Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Gly Ala Lys Ala Gln 
            420         


<210>  5
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S-013


<220>
<221>  misc_feature
<222>  (2)..(1270)
<223>  mutant EgD8S-013 delta-8 desaturase CDS

<400>  5
catggtgaag gcttctcgac aggctctgcc cctcgtcatc gacggaaagg tgtacgacgt     60

ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact accagggacg    120

agatgctact gacgccttca tggttatgca ctctcaggaa ggctacgaca agctcaagcg    180

aatgcccaag atcaaccagg cttccgagct gcctccccag gctgccgtca acgaagctca    240

ggaggatttc cgaaagctcc gagaagagct gatcgccact ggcatgtttg acgcctctcc    300

cctctggtac tcgtacaaga tcttgaccac cctgggtctt ggcgtgcttg ccttcttcat    360

gctggtccag taccacctgt acttcattgg tgctctcgtg ctcggtatgc actaccagca    420

aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc gaaactggaa    480

taacgtcctg ggtctggtct ttggcaacgc tgtccagggc ttctccgtga cctggtggaa    540

ggacagacac aacgcccatc attctgctac caacgttcag ggtcacgatc ccgacattga    600

taacctgcct ctgctcgcct ggtccgagga cgatgtcact cgagcttctc ccatctcccg    660

aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat    720

ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta    780

ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc tcaagaccct    840

gttccacctc ttctttatgc cctccatcct gacctcgatg ctggtgttct ttgtttccga    900

gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg aaccactacc ctctggagaa    960

gatcggtgat tccgtctggg acggacatgg cttctctgtg ggtcagatcc atgagaccat   1020

gaacattcga cgaggcatca ttactgactg gttctttgga ggcctgaact accagatcga   1080

gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga   1140

acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg aaggtctcgt   1200

catcctgctc cgatacctgt cccagttcgc tcgaatggcc gagaagcagc ccggtgccaa   1260

ggctcagtaa gc                                                       1272


<210>  6
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant EgD8S-013, comprising M1, M2, M3, M8, M12, M15, M16, M18, 
       M21, M26, M38, M45, M46, M68, M69 and M70 mutation sites

<400>  6

Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys 
1               5                   10                  15      


Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Gly Tyr Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe 
        115                 120                 125             


Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Val Leu Gly Leu Val Phe Gly Asn Ala Val Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Gly Ala Lys Ala Gln 
            420         


<210>  7
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S-015


<220>
<221>  misc_feature
<222>  (2)..(1270)
<223>  mutant EgD8S-015 delta-8 desaturase CDS

<400>  7
catggtgaag gcttctcgac aggctctgcc cctcgtcatc gacggaaagg tgtacgacgt     60

ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact accagggacg    120

agatgctact gacgccttca tggttatgca ctctcaggaa ggctacgaca agctcaagcg    180

aatgcccaag atcaaccagg cttccgagct gcctccccag gctgccgtca acgaagctca    240

ggaggatttc cgaaagctcc gagaagagct gatcgccact ggcatgtttg acgcctctcc    300

cctctggtac tcgtacaaga tcttgaccac cctgggtctt ggcgtgcttg ccttcttcat    360

gctggtccag taccacctgt acttcattgg tgctgtgctg ctcggtatgc actaccagca    420

aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc gaaactggaa    480

taacgtcctg ggtctggtct ttggcaacgc tgtccagggc ttctccgtga cctggtggaa    540

ggacagacac aacgcccatc attctgctac caacgttcag ggtcacgatc ccgacattga    600

taacctgcct ctgctcgcct ggtccgagga cgatgtcact cgagcttctc ccatctcccg    660

aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat    720

ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta    780

ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc tcaagctgac    840

cttccacctc ttctttatgc cctccatcct gacctcgatg ctggtgttct ttgtttccga    900

gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg aaccactacc ctctggagaa    960

gatcggtgat tccgtctggg acggacatgg cttctctgtg ggtcagatcc atgagaccat   1020

gaacattcga cgaggcgtcc tctctgactg gttctttgga ggcctgaact accagatcga   1080

gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga   1140

acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg aaggtctcgt   1200

catcctgctc cgatacctgt cccagttcgc tcgaatggcc gagaagcagc ccggtgccaa   1260

ggctcagtaa gc                                                       1272


<210>  8
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S-015 comprising M1, M2, M3, M8, M12, M15, M16, M18, 
       M21, M26, M38, M45, M51, M63, M68, M69 and M70 mutation sites

<400>  8

Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys 
1               5                   10                  15      


Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Gly Tyr Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe 
        115                 120                 125             


Ile Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Val Leu Gly Leu Val Phe Gly Asn Ala Val Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Leu Thr Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Val Leu Ser Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Gly Ala Lys Ala Gln 
            420         


<210>  9
<211>  1272
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (2)..(1270)
<223>  synthetic delta-8 desaturase CDS, codon-optimized for Yarrowia 
       lipolytica ("D8SF" or "EgD8S")

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2006/012325 and WO 2006/012326
<311>  2005-06-24
<312>  2006-02-02
<313>  (1)..(1272)

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US-2005-0287652-A1
<311>  2005-06-24
<312>  2005-12-29
<313>  (1)..(1272)

<400>  9
catggtgaag tccaagcgac aggctctgcc cctcaccatc gacggaacta cctacgacgt     60

ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact accagggacg    120

agatgctact gacgccttca tggttatgca ctctcaggaa gccttcgaca agctcaagcg    180

aatgcccaag atcaacccct cctccgagct gcctccccag gctgccgtca acgaagctca    240

ggaggatttc cgaaagctcc gagaagagct gatcgccact ggcatgtttg acgcctctcc    300

cctctggtac tcgtacaaga tctccaccac cctgggtctt ggcgtgcttg gatacttcct    360

gatggtccag taccagatgt acttcattgg tgctgtgctg ctcggtatgc actaccagca    420

aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc gaaactggaa    480

taacctcgtg ggtctggtct ttggcaacgg actccagggc ttctccgtga cctggtggaa    540

ggacagacac aacgcccatc attctgctac caacgttcag ggtcacgatc ccgacattga    600

taacctgcct ctgctcgcct ggtccgagga cgatgtcact cgagcttctc ccatctcccg    660

aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat    720

ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta    780

ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc tcaagaccct    840

gttccacctc ttctttatgc cctccatcct gacctcgctc ctggtgttct ttgtttccga    900

gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg aaccactacc ctctggagaa    960

gatcggtgat tccgtctggg acggacatgg cttctctgtg ggtcagatcc atgagaccat   1020

gaacattcga cgaggcatca ttactgactg gttctttgga ggcctgaact accagatcga   1080

gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga   1140

acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg aaggtctcgt   1200

catcctgctc cgatacctgg ccgtgttcgc tcgaatggcc gagaagcagc ccgctggcaa   1260

ggctctctaa gc                                                       1272


<210>  10
<211>  422
<212>  PRT
<213>  Euglena gracilis


<220>
<221>  MISC_FEATURE
<223>  synthetic delta-8 desaturase codon-optimized for Yarrowia 
       lipolytica ("D8SF" or "EgD8S")

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2006/012325 and WO 2006/012326
<311>  2005-06-24
<312>  2006-02-02
<313>  (1)..(422)

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US-2005-0287652-A1
<311>  2005-06-24
<312>  2005-12-29
<313>  (1)..(422)

<400>  10

Met Val Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr 
1               5                   10                  15      


Thr Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe 
        115                 120                 125             


Ile Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Ala Gly Lys Ala Leu 
            420         


<210>  11
<211>  1271
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (4)..(1269)
<223>  delta-8 desaturase ("Eg5" or "EgD8") CDS

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2006/012325 and WO 2006/012326
<311>  2005-06-24
<312>  2006-02-02
<313>  (1)..(1271)

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US-2005-0287652-A1
<311>  2005-06-24
<312>  2005-12-29
<313>  (1)..(1271)

<400>  11
gaaatgaagt caaagcgcca agcgcttccc cttacaattg atggaacaac atatgatgtg     60

tctgcctggg tcaatttcca ccctggtggt gcggaaatta tagagaatta ccaaggaagg    120

gatgccactg atgccttcat ggttatgcac tctcaagaag ccttcgacaa gctcaagcgc    180

atgcccaaaa tcaatcccag ttctgagttg ccaccccagg ctgcagtgaa tgaagctcaa    240

gaggatttcc ggaagctccg agaagagttg atcgcaactg gcatgtttga tgcctccccc    300

ctctggtact catacaaaat cagcaccaca ctgggccttg gagtgctggg ttatttcctg    360

atggttcagt atcagatgta tttcattggg gcagtgttgc ttgggatgca ctatcaacag    420

atgggctggc tttctcatga catttgccac caccagactt tcaagaaccg gaactggaac    480

aacctcgtgg gactggtatt tggcaatggt ctgcaaggtt tttccgtgac atggtggaag    540

gacagacaca atgcacatca ttcggcaacc aatgttcaag ggcacgaccc tgatattgac    600

aacctccccc tcttagcctg gtctgaggat gacgtcacac gggcgtcacc gatttcccgc    660

aagctcattc agttccagca gtactatttc ttggtcatct gtatcttgtt gcggttcatt    720

tggtgtttcc agagcgtgtt gaccgtgcgc agtttgaagg acagagataa ccaattctat    780

cgctctcagt ataagaagga ggccattggc ctcgccctgc actggacctt gaagaccctg    840

ttccacttat tctttatgcc cagcatcctc acatcgctgt tggtgttttt cgtttcggag    900

ctggttggcg gcttcggcat tgcgatcgtg gtgttcatga accactaccc actggagaag    960

atcggggact cagtctggga tggccatgga ttctcggttg gccagatcca tgagaccatg   1020

aacattcggc gagggattat cacagattgg tttttcggag gcttgaatta ccagattgag   1080

caccatttgt ggccgaccct ccctcgccac aacctgacag cggttagcta ccaggtggaa   1140

cagctgtgcc agaagcacaa cctgccgtat cggaacccgc tgccccatga agggttggtc   1200

atcctgctgc gctatctggc ggtgttcgcc cggatggcgg agaagcaacc cgcggggaag   1260

gctctataag g                                                        1271


<210>  12
<211>  421
<212>  PRT
<213>  Euglena gracilis


<220>
<221>  MISC_FEATURE
<223>  delta-8 desaturase ("Eg5" or "EgD8")

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2006/012325 and WO 2006/012326
<311>  2005-06-24
<312>  2006-02-02
<313>  (1)..(421)

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US_2005-0287652-A1
<311>  2005-06-24
<312>  2005-12-29
<313>  (1)..(421)

<400>  12

Met Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr Thr 
1               5                   10                  15      


Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu Ile 
            20                  25                  30          


Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val Met 
        35                  40                  45              


His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile Asn 
    50                  55                  60                  


Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu 
65                  70                  75                  80  


Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp 
                85                  90                  95      


Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly Leu 
            100                 105                 110         


Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe Ile 
        115                 120                 125             


Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu Ser 
    130                 135                 140                 


His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn 
145                 150                 155                 160 


Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val Thr 
                165                 170                 175     


Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val Gln 
            180                 185                 190         


Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu 
        195                 200                 205             


Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe 
    210                 215                 220                 


Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp 
225                 230                 235                 240 


Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp Asn 
                245                 250                 255     


Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu 
            260                 265                 270         


His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser Ile 
        275                 280                 285             


Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly Phe 
    290                 295                 300                 


Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile 
305                 310                 315                 320 


Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His 
                325                 330                 335     


Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly 
            340                 345                 350         


Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg 
        355                 360                 365             


His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys 
    370                 375                 380                 


His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val Ile 
385                 390                 395                 400 


Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln Pro 
                405                 410                 415     


Ala Gly Lys Ala Leu 
            420     


<210>  13
<211>  467
<212>  PRT
<213>  Amylomyces rouxii (GenBank Accession No. AAR27297)

<400>  13

Met Ser Ser Asp Val Gly Ala Thr Val Pro His Phe Tyr Thr Arg Ala 
1               5                   10                  15      


Glu Leu Ala Asp Ile His Gln Asp Val Leu Asp Lys Lys Pro Glu Ala 
            20                  25                  30          


Arg Lys Leu Ile Val Val Glu Asn Lys Val Tyr Asp Ile Thr Asp Phe 
        35                  40                  45              


Val Phe Asp His Pro Gly Gly Glu Arg Val Leu Leu Thr Gln Glu Gly 
    50                  55                  60                  


Arg Asp Ala Thr Asp Val Phe His Glu Met His Pro Pro Ser Ala Tyr 
65                  70                  75                  80  


Glu Leu Leu Ala Asn Cys Tyr Val Gly Asp Cys Glu Pro Lys Leu Pro 
                85                  90                  95      


Ile Asp Ser Thr Asp Lys Lys Ala Leu Asn Ser Ala Ala Phe Ala Gln 
            100                 105                 110         


Glu Ile Arg Asp Leu Arg Asp Lys Leu Glu Lys Gln Gly Tyr Phe Asp 
        115                 120                 125             


Ala Ser Thr Gly Phe Tyr Ile Tyr Lys Val Ser Thr Thr Leu Leu Val 
    130                 135                 140                 


Cys Ile Val Gly Leu Ala Ile Leu Lys Ala Trp Gly Arg Glu Ser Thr 
145                 150                 155                 160 


Leu Ala Val Phe Ile Ala Ala Ser Leu Val Gly Leu Phe Trp Gln Gln 
                165                 170                 175     


Cys Gly Trp Leu Ala His Asp Tyr Ala His Tyr Gln Val Ile Lys Asp 
            180                 185                 190         


Pro Asn Val Asn Asn Leu Phe Leu Val Thr Phe Gly Asn Leu Val Gln 
        195                 200                 205             


Gly Phe Ser Leu Ser Trp Trp Lys Asn Lys His Asn Thr His His Ala 
    210                 215                 220                 


Ser Thr Asn Val Ser Gly Glu Asp Pro Asp Ile Asp Thr Ala Pro Ile 
225                 230                 235                 240 


Leu Leu Trp Asp Glu Phe Ala Val Ala Asn Phe Tyr Gly Ser Leu Lys 
                245                 250                 255     


Asp Asn Ala Ser Gly Phe Asp Arg Phe Ile Ala Glu His Ile Leu Pro 
            260                 265                 270         


Tyr Gln Thr Arg Tyr Tyr Phe Phe Ile Leu Gly Phe Ala Arg Thr Ser 
        275                 280                 285             


Trp Ala Ile Gln Ser Ile Ile Tyr Ser Phe Lys Asn Glu Thr Leu Asn 
    290                 295                 300                 


Lys Ser Lys Leu Leu Ser Trp Cys Glu Arg Ile Phe Leu Ile Val His 
305                 310                 315                 320 


Trp Val Phe Phe Thr Tyr Cys Thr Ile Ala Trp Ile Ser Ser Ile Arg 
                325                 330                 335     


Asn Ile Ala Met Phe Phe Val Val Ser Gln Ile Thr Thr Gly Tyr Leu 
            340                 345                 350         


Leu Ala Ile Val Phe Ala Met Asn His Asn Gly Met Pro Val Tyr Ser 
        355                 360                 365             


Pro Glu Glu Ala Asn His Thr Glu Phe Tyr Glu Leu Gln Cys Ile Thr 
    370                 375                 380                 


Gly Arg Asp Val Asn Cys Thr Val Phe Gly Asp Trp Leu Met Gly Gly 
385                 390                 395                 400 


Leu Asn Tyr Gln Ile Glu His His Leu Phe Pro Glu Met Pro Arg His 
                405                 410                 415     


His Leu Ser Lys Val Lys Ser Met Val Lys Pro Ile Ala Gln Lys Tyr 
            420                 425                 430         


Asn Ile Pro Tyr His Asp Thr Thr Val Ile Gly Gly Thr Ile Glu Val 
        435                 440                 445             


Leu Gln Thr Leu Asp Phe Val Gln Lys Ile Ser Gln Lys Phe Ser Lys 
    450                 455                 460                 


Lys Met Leu 
465         


<210>  14
<211>  445
<212>  PRT
<213>  Rhizopus oryzae (GenBank Accession No. AAS93682)

<400>  14

Met Ser Thr Ser Asp Arg Gln Ser Val Phe Thr Leu Lys Glu Leu Glu 
1               5                   10                  15      


Leu Ile Asn Gln Lys His Arg Asp Gly Asp Lys Ser Ala Met Lys Phe 
            20                  25                  30          


Ile Ile Ile Asp Arg Lys Val Tyr Asp Val Thr Glu Phe Leu Glu Asp 
        35                  40                  45              


His Gly Gly Ala Gln Val Leu Leu Thr His Val Gly Lys Asp Ala Ser 
    50                  55                  60                  


Asp Val Phe His Ala Met His Glu Ser Ala Tyr Glu Ile Leu Asn Asn 
65                  70                  75                  80  


Tyr Phe Val Gly Asp Val Lys Asp Ala His Val Lys Glu Thr Ser Ala 
                85                  90                  95      


Gln Phe Ala Ser Glu Met Arg Gln Leu Arg Asp Gln Leu Lys Lys Glu 
            100                 105                 110         


Gly Tyr Phe His Ser Ser Lys Ala Tyr Tyr Val Tyr Lys Val Leu Ser 
        115                 120                 125             


Thr Leu Ala Leu Cys Ala Ala Gly Leu Thr Leu Leu Tyr Ala Tyr Gly 
    130                 135                 140                 


His Thr Ser Thr Leu Ala Val Val Ala Ser Ala Ile Ile Val Gly Ile 
145                 150                 155                 160 


Phe Trp Gln Gln Cys Gly Trp Leu Ala His Asp Phe Gly His His Gln 
                165                 170                 175     


Cys Phe Glu Asp Arg Ser Trp Asn Asp Val Leu Val Val Phe Leu Gly 
            180                 185                 190         


Asn Phe Cys Gln Gly Phe Ser Leu Ser Trp Trp Lys Asn Lys His Asn 
        195                 200                 205             


Thr His His Ala Ser Thr Asn Val His Gly His Asp Asp Ile Asp Thr 
    210                 215                 220                 


Ala Val Leu Leu Trp Asp Glu Tyr Ala Ser Ala Ala Tyr Tyr Ala Ser 
225                 230                 235                 240 


Leu Asp Glu Glu Thr Met Ile Ser Arg Phe Leu Ala Glu Ser Val Leu 
                245                 250                 255     


His Gln Thr Arg Tyr Tyr Phe Phe Val Leu Gly Phe Ala Arg Leu Ser 
            260                 265                 270         


Trp Ala Ile Gln Ser Leu Leu Tyr Ser Phe Lys Gln Gly Ala Ile Asn 
        275                 280                 285             


Lys Ser His Gln Leu Asn Leu Phe Glu Arg Phe Cys Leu Val Ser His 
    290                 295                 300                 


Trp Thr Leu Phe Thr Tyr Cys Thr Leu Ala Trp Cys Ser Asn Val Tyr 
305                 310                 315                 320 


His Met Ile Leu Phe Phe Leu Val Ser Gln Ala Thr Thr Gly Tyr Thr 
                325                 330                 335     


Leu Ala Leu Val Phe Ala Leu Asn His Asn Gly Met Val Ile Thr Glu 
            340                 345                 350         


Glu Lys Ala Glu Ser Met Glu Phe Phe Glu Ile Gln Val Ile Thr Gly 
        355                 360                 365             


Arg Asp Val Thr Leu Ser Leu Gly Asp Trp Phe Met Gly Gly Leu Asn 
    370                 375                 380                 


Tyr Gln Ile Glu His His Val Phe Asn Met Arg His Asn Leu Lys Val 
385                 390                 395                 400 


Lys Met Val Lys Ser Leu Cys Lys Lys Tyr Asp Ile Asn Tyr His Asp 
                405                 410                 415     


Thr Gly Phe Leu Lys Gly Thr Leu Glu Val Leu Lys Thr Leu Asp Ile 
            420                 425                 430         


Thr Ser Lys Leu Ser Leu Gln Leu Ser Lys Lys Ser Phe 
        435                 440                 445 


<210>  15
<211>  382
<212>  PRT
<213>  Leishmania major (GenBank Accession No. CAJ09677)

<400>  15

Met Val Phe Glu Leu Thr Tyr Lys Tyr His Ser Ser Leu Lys Cys Val 
1               5                   10                  15      


Ile Arg Ile Asp Asp Asn Trp Tyr Asp Cys Thr Ser Trp Arg Asn Ser 
            20                  25                  30          


His Gly Gly Ala Gln Met Cys Asp Glu Phe His Gly Lys Asp Ala Thr 
        35                  40                  45              


Asp Ala Phe Tyr Ser Leu His Ser Lys Glu Ala Ile Gln Lys Leu Lys 
    50                  55                  60                  


Arg Met Lys Ala Leu Leu Lys Glu Gly Asp Glu Arg Asp Gln Val Ser 
65                  70                  75                  80  


Leu Asn Phe Glu Lys Leu Leu Gln Gln Leu Arg Ser Glu Gly Trp Phe 
                85                  90                  95      


Glu Arg Arg Trp Ile Ile Asp Phe Ala Arg Asn Ile Met Val Ile Val 
            100                 105                 110         


Leu Cys Val Val Gly Thr Tyr Leu Ser Tyr Ser Arg Phe Leu Ala Thr 
        115                 120                 125             


Ile Leu Ile Gly Leu Gly Met Gln Gln Gly Gly Trp Leu Ala His Asp 
    130                 135                 140                 


Phe Thr His Ala Arg Gly Lys Phe Ala Arg Phe Leu Ala Asn Ala Cys 
145                 150                 155                 160 


Gly Gly Met Ile Asn Ala Phe Ser Val Glu Trp Trp Ser Asn Lys His 
                165                 170                 175     


Asn Ser His His Ile Phe Val Asn Arg Lys Gly Met Asp Ala Asp Ile 
            180                 185                 190         


His Asn Glu Ala Leu Phe Leu Trp Val Asp Val Ser Glu Asp Thr Ala 
        195                 200                 205             


Cys Arg Arg Tyr Gln Tyr Thr Phe Tyr Leu Ala Ala Tyr Ala Leu Leu 
    210                 215                 220                 


Tyr Val Ser Trp Arg Ile Gln Ser Leu Arg Phe Ala Leu Gly Ser Gly 
225                 230                 235                 240 


Asn Arg Leu Glu Leu Ser Leu Met Ala Leu Asn Tyr Leu Trp Leu Ala 
                245                 250                 255     


Leu Leu Trp Arg Val Ser Leu Gly Ser Val Leu Leu Gly Gly Phe Phe 
            260                 265                 270         


Val Ala Val Val Val Thr Val Asn His Gln Thr Glu Glu Met Ile Glu 
        275                 280                 285             


His Asp Glu Tyr Asn Tyr Val Val Asp Gln His Arg Ala Thr Arg Gly 
    290                 295                 300                 


Val His Cys Ser Asp Phe Phe Glu Trp Phe Phe Gly Gly Met Gln Tyr 
305                 310                 315                 320 


Gln Leu Glu His His Leu Leu Met Val Arg Tyr Arg Tyr Glu Val Arg 
                325                 330                 335     


Arg Leu Leu Arg Lys Phe Ser Glu Asp Asn Gly Leu Phe His Val Glu 
            340                 345                 350         


Gly Val Trp Lys Ile Thr Lys Met Asn Phe Asp Ile Leu Tyr Ser Ile 
        355                 360                 365             


Ser Thr Thr Ala Leu Asn Ser Ala Glu Ala Lys Ser Gly Lys 
    370                 375                 380         


<210>  16
<211>  439
<212>  PRT
<213>  Mortierella isabellina (GenBank Accession No. AAG38104)

<400>  16

Met Ala Ala Ala Ser Val Arg Thr Phe Thr Arg Ala Glu Ile Leu Asn 
1               5                   10                  15      


Ala Glu Ala Leu Asn Glu Gly Lys Lys Asp Ala Glu Ala Phe Leu Met 
            20                  25                  30          


Ile Ile Asp Asn Lys Val Tyr Asp Val Arg Glu Phe Val Asp His Gly 
        35                  40                  45              


Gly Ser Val Ile Leu Thr His Val Gly Lys Asp Gly Thr Asp Val Phe 
    50                  55                  60                  


Asp Thr Phe His Glu Ala Ala Trp Glu Thr Leu Ala Asn Phe Tyr Val 
65                  70                  75                  80  


Gly Asp Ile Asp Glu Ser Asp Arg Ala Ile Lys Asn Asp Asp Phe Ala 
                85                  90                  95      


Ala Glu Val Arg Lys Leu Arg Thr Leu Phe Gln Ser Leu Gly Tyr Tyr 
            100                 105                 110         


Asp Ser Ser Lys Ala Tyr Tyr Ala Phe Lys Val Ser Phe Asn Leu Cys 
        115                 120                 125             


Ile Trp Gly Leu Ser Thr Phe Ile Val Ala Lys Trp Gly Gln Thr Ser 
    130                 135                 140                 


Thr Leu Ala Asn Val Leu Ser Ala Ala Leu Leu Gly Leu Phe Trp Gln 
145                 150                 155                 160 


Gln Cys Gly Trp Leu Ala His Asp Phe Leu His His Gln Val Phe Gln 
                165                 170                 175     


Asp Arg Phe Trp Gly Asp Leu Phe Gly Ala Phe Leu Gly Gly Val Cys 
            180                 185                 190         


Gln Gly Phe Ser Ser Ser Trp Trp Lys Asp Lys His Asn Thr His His 
        195                 200                 205             


Ala Ala Asn Val His Gly Glu Asp Asp Ile Asp Thr His Leu Leu Thr 
    210                 215                 220                 


Trp Ser Glu His Ala Leu Glu Met Phe Ser Asp Val Asp Glu Glu Leu 
225                 230                 235                 240 


Thr Arg Met Trp Ser Arg Phe Met Val Leu Asn Gln Thr Trp Phe Tyr 
                245                 250                 255     


Phe Ile Leu Ser Phe Ala Arg Leu Ser Trp Cys Leu Gln Ser Ile Leu 
            260                 265                 270         


Leu Val Leu Asn Gly Gln Ala His Lys Ser Gly Ala Arg Val Ser Ile 
        275                 280                 285             


Ser Leu Val Glu Gln Leu Ser Leu Ala Met His Trp Thr Trp Tyr Leu 
    290                 295                 300                 


Ala Thr Met Phe Leu Phe Ile Lys Asp Val Asn Met Met Val Tyr Phe 
305                 310                 315                 320 


Leu Val Ser Gln Ala Val Cys Gly Asn Leu Leu Ala Ile Val Phe Ser 
                325                 330                 335     


Leu Asn His Asn Gly Met Val Ile Ser Lys Glu Glu Ala Val Asp Met 
            340                 345                 350         


Asp Phe Phe Thr Lys Gln Ile Ile Thr Gly Arg Asp Val His Gly Leu 
        355                 360                 365             


Phe Ala Asn Trp Phe Thr Gly Gly Leu Asn Tyr Gln Ile Glu His His 
    370                 375                 380                 


Leu Phe Ser Met Arg His Asn Phe Ser Lys Ile Gln Ala Val Glu Thr 
385                 390                 395                 400 


Leu Cys Lys Lys Tyr Gly Val Arg Tyr His Thr Thr Gly Met Ile Glu 
                405                 410                 415     


Gly Thr Ala Glu Val Phe Ser Arg Leu Asn Glu Val Ser Lys Ala Ala 
            420                 425                 430         


Ser Lys Met Gly Lys Ala Gln 
        435                 


<210>  17
<211>  14655
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKLeuN-29E3


<220>
<221>  misc_feature
<222>  (8822)..(8822)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (8827)..(8830)
<223>  n is a, c, g, or t

<400>  17
cgattgttgt ctactaacta tcgtacgata acttcgtata gcatacatta tacgaagtta     60

tcgcgtcgac gagtatctgt ctgactcgtc attgccgcct ttggagtacg actccaacta    120

tgagtgtgct tggatcactt tgacgataca ttcttcgttg gaggctgtgg gtctgacagc    180

tgcgttttcg gcgcggttgg ccgacaacaa tatcagctgc aacgtcattg ctggctttca    240

tcatgatcac atttttgtcg gcaaaggcga cgcccagaga gccattgacg ttctttctaa    300

tttggaccga tagccgtata gtccagtcta tctataagtt caactaactc gtaactatta    360

ccataacata tacttcactg ccccagataa ggttccgata aaaagttctg cagactaaat    420

ttatttcagt ctcctcttca ccaccaaaat gccctcctac gaagctcgag ctaacgtcca    480

caagtccgcc tttgccgctc gagtgctcaa gctcgtggca gccaagaaaa ccaacctgtg    540

tgcttctctg gatgttacca ccaccaagga gctcattgag cttgccgata aggtcggacc    600

ttatgtgtgc atgatcaaaa cccatatcga catcattgac gacttcacct acgccggcac    660

tgtgctcccc ctcaaggaac ttgctcttaa gcacggtttc ttcctgttcg aggacagaaa    720

gttcgcagat attggcaaca ctgtcaagca ccagtaccgg tgtcaccgaa tcgccgagtg    780

gtccgatatc accaacgccc acggtgtacc cggaaccgga atcattgctg gcctgcgagc    840

tggtgccgag gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc    900

ccagtacaag gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat    960

gctggccgag ctgtcttgca agggctctct ggccactggc gagtactcca agcagaccat   1020

tgagcttgcc cgatccgacc ccgagtttgt ggttggcttc attgcccaga accgacctaa   1080

gggcgactct gaggactggc ttattctgac ccccggggtg ggtcttgacg acaagggaga   1140

cgctctcgga cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat   1200

aattgtcggc cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata   1260

ccagaaggct ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata   1320

tgtaatttaa ctgtgtatat agagagcgtg caagtatgga gcgcttgttc agcttgtatg   1380

atggtcagac gacctgtctg atcgagtatg tatgatactg cacaacctgt gtatccgcat   1440

gatctgtcca atggggcatg ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct   1500

aatacgttga actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt   1560

attctcaact acatccccag tcacaatacc accactgcac taccactaca ccaaaaccat   1620

gatcaaacca cccatggact tcctggaggc agaagaactt gttatggaaa agctcaagag   1680

agagatcata acttcgtata gcatacatta tacgaagtta tcctgcaggt aaaggaattc   1740

tggagtttct gagagaaaaa ggcaagatac gtatgtaaca aagcgacgca tggtacaata   1800

ataccggagg catgtatcat agagagttag tggttcgatg atggcactgg tgcctggtat   1860

gactttatac ggctgactac atatttgtcc tcagacatac aattacagtc aagcacttac   1920

ccttggacat ctgtaggtac cccccggcca agacgatctc agcgtgtcgt atgtcggatt   1980

ggcgtagctc cctcgctcgt caattggctc ccatctactt tcttctgctt ggctacaccc   2040

agcatgtctg ctatggctcg ttttcgtgcc ttatctatcc tcccagtatt accaactcta   2100

aatgacatga tgtgattggg tctacacttt catatcagag ataaggagta gcacagttgc   2160

ataaaaagcc caactctaat cagcttcttc ctttcttgta attagtacaa aggtgattag   2220

cgaaatctgg aagcttagtt ggccctaaaa aaatcaaaaa aagcaaaaaa cgaaaaacga   2280

aaaaccacag ttttgagaac agggaggtaa cgaaggatcg tatatatata tatatatata   2340

tatacccacg gatcccgaga ccggcctttg attcttccct acaaccaacc attctcacca   2400

ccctaattca caaccatgga gtctggaccc atgcctgctg gcattccctt ccctgagtac   2460

tatgacttct ttatggactg gaagactccc ctggccatcg ctgccaccta cactgctgcc   2520

gtcggtctct tcaaccccaa ggttggcaag gtctcccgag tggttgccaa gtcggctaac   2580

gcaaagcctg ccgagcgaac ccagtccgga gctgccatga ctgccttcgt ctttgtgcac   2640

aacctcattc tgtgtgtcta ctctggcatc accttctact acatgtttcc tgctatggtc   2700

aagaacttcc gaacccacac actgcacgaa gcctactgcg acacggatca gtccctctgg   2760

aacaacgcac ttggctactg gggttacctc ttctacctgt ccaagttcta cgaggtcatt   2820

gacaccatca tcatcatcct gaagggacga cggtcctcgc tgcttcagac ctaccaccat   2880

gctggagcca tgattaccat gtggtctggc atcaactacc aagccactcc catttggatc   2940

tttgtggtct tcaactcctt cattcacacc atcatgtact gttactatgc cttcacctct   3000

atcggattcc atcctcctgg caaaaagtac ctgacttcga tgcagattac tcagtttctg   3060

gtcggtatca ccattgccgt gtcctacctc ttcgttcctg gctgcatccg aacacccggt   3120

gctcagatgg ctgtctggat caacgtcggc tacctgtttc ccttgaccta tctgttcgtg   3180

gactttgcca agcgaaccta ctccaagcga tctgccattg ccgctcagaa aaaggctcag   3240

taagcggccg cattgatgat tggaaacaca cacatgggtt atatctaggt gagagttagt   3300

tggacagtta tatattaaat cagctatgcc aacggtaact tcattcatgt caacgaggaa   3360

ccagtgactg caagtaatat agaatttgac caccttgcca ttctcttgca ctcctttact   3420

atatctcatt tatttcttat atacaaatca cttcttcttc ccagcatcga gctcggaaac   3480

ctcatgagca ataacatcgt ggatctcgtc aatagagggc tttttggact ccttgctgtt   3540

ggccaccttg tccttgctgt ctggctcatt ctgtttcaac gccttttaat taacggagta   3600

ggtctcggtg tcggaagcga cgccagatcc gtcatcctcc tttcgctctc caaagtagat   3660

acctccgacg agctctcgga caatgatgaa gtcggtgccc tcaacgtttc ggatggggga   3720

gagatcggcg agcttgggcg acagcagctg gcagggtcgc aggttggcgt acaggttcag   3780

gtcctttcgc agcttgagga gaccctgctc gggtcgcacg tcggttcgtc cgtcgggagt   3840

ggtccatacg gtgttggcag cgcctccgac agcaccgagc ataatagagt cagcctttcg   3900

gcagatgtcg agagtagcgt cggtgatggg ctcgccctcc ttctcaatgg cagctcctcc   3960

aatgagtcgg tcctcaaaca caaactcggt gccggaggcc tcagcaacag acttgagcac   4020

cttgacggcc tcggcaatca cctcggggcc acagaagtcg ccgccgagaa gaacaatctt   4080

cttggagtca gtcttggtct tcttagtttc gggttccatt gtggatgtgt gtggttgtat   4140

gtgtgatgtg gtgtgtggag tgaaaatctg tggctggcaa acgctcttgt atatatacgc   4200

acttttgccc gtgctatgtg gaagactaaa cctccgaaga ttgtgactca ggtagtgcgg   4260

tatcggctag ggacccaaac cttgtcgatg ccgatagcat gcgacgtcgg gcccaattcg   4320

ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa   4380

aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt   4440

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa   4500

tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga   4560

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg   4620

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat   4680

ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg   4740

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata   4800

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt   4860

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat   4920

ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct   4980

tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg   5040

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat   5100

aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc   5160

gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa   5220

cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac   5280

tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga   5340

tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag   5400

agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca   5460

cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca   5520

tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa   5580

ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc   5640

tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa   5700

cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag   5760

actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct   5820

ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac   5880

tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa   5940

ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt   6000

aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat   6060

ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg   6120

agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc   6180

ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg   6240

tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag   6300

cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact   6360

ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg   6420

gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc   6480

ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg   6540

aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg   6600

cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag   6660

ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc   6720

gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct   6780

ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc   6840

ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc   6900

gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac   6960

cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcgcgcccac tgagctcgtc   7020

taacggactt gatatacaac caattaaaac aaatgaaaag aaatacagtt ctttgtatca   7080

tttgtaacaa ttaccctgta caaactaagg tattgaaatc ccacaatatt cccaaagtcc   7140

acccctttcc aaattgtcat gcctacaact catataccaa gcactaacct accaaacacc   7200

actaaaaccc cacaaaatat atcttaccga atatacagta acaagctacc accacactcg   7260

ttgggtgcag tcgccagctt aaagatatct atccacatca gccacaactc ccttccttta   7320

ataaaccgac tacacccttg gctattgagg ttatgagtga atatactgta gacaagacac   7380

tttcaagaag actgtttcca aaacgtacca ctgtcctcca ctacaaacac acccaatctg   7440

cttcttctag tcaaggttgc tacaccggta aattataaat catcatttca ttagcagggc   7500

agggcccttt ttatagagtc ttatacacta gcggaccctg ccggtagacc aacccgcagg   7560

cgcgtcagtt tgctccttcc atcaatgcgt cgtagaaacg acttactcct tcttgagcag   7620

ctccttgacc ttgttggcaa caagtctccg acctcggagg tggaggaaga gcctccgata   7680

tcggcggtag tgataccagc ctcgacggac tccttgacgg cagcctcaac agcgtcaccg   7740

gcgggcttca tgttaagaga gaacttgagc atcatggcgg cagacagaat ggtggcgtac   7800

gcaactaaca tgaatgaata cgatatacat caaagactat gatacgcagt attgcacact   7860

gtacgagtaa gagcactagc cactgcactc aagtgaaacc gttgcccggg tacgagtatg   7920

agtatgtaca gtatgtttag tattgtactt ggacagtgct tgtatcgtac attctcaagt   7980

gtcaaacata aatatccgtt gctatatcct cgcaccacca cgtagctcgc tatatccctg   8040

tgttgaatcc atccatcttg gattgccaat tgtgcacaca gaaccgggca ctcacttccc   8100

catccacact tgcggccgcg cctacttaag caacgggctt gataacagcg gggggggtgc   8160

ccacgttgtt gcggttgcgg aagaacagaa cacccttacc agcaccctcg gcaccagcgc   8220

tgggctcaac ccactggcac atacgcgcac tgcggtacat ggcgcggatg aagccacgag   8280

gaccatcctg gacatcagcc cggtagtgct tgcccatgat gggcttaatg gcctcggtgg   8340

cctcgtccgc gttgtagaag gggatgctgc tgacgtagtg gtggaggaca tgagtctcga   8400

tgatgccgtg gagaaggtgg cggccgatga agcccatctc acggtcaatg gtagcagcgg   8460

caccacggac gaagttccac tcgtcgttgg tgtagtgggg aagggtaggg tcggtgtgct   8520

ggaggaaggt gatggcaacg agccagtggt taacccagag gtagggaaca aagtaccaga   8580

tggccatgtt gtagaaaccg aacttctgaa cgaggaagta cagagcagtg gccatcagac   8640

cgataccaat atcgctgagg acgatgagct tagcgtcact gttctcgtac agagggctgc   8700

ggggatcgaa gtggttaaca ccaccgccga ggccgttatg cttgcccttg ccgcgaccct   8760

cacgctggcg ctcgtggtag ttgtggccgg taacattggt gatgaggtag ttgggccagc   8820

cnacgannnn ctcagtaaga tgagcgagct cgtgggtcat ctttccgaga cgagtagcct   8880

gctgctcgcg ggttcgggga acgaagacca tgtcacgctc catgttgcca gtggccttgt   8940

ggtgctttcg gtgggagatt tgccagctga agtaggggac aaggagggaa gagtgaagaa   9000

cccagccagt aatgtcgttg atgatgcgag aatcggagaa agcaccgtga ccgcactcat   9060

gggcaataac ccagagacca gtaccgaaaa gaccctgaag aacggtgtac acggcccaca   9120

gaccagcgcg ggcgggggtg gaggggatat attcgggggt cacaaagttg taccagatgc   9180

tgaaagtggt agtcaggagg acaatgtcgc ggaggatata accgtatccc ttgagagcgg   9240

agcgcttgaa gcagtgctta gggatggcat tgtagatgtc cttgatggta aagtcgggaa   9300

cctcgaactg gttgccgtag gtgtcgagca tgacaccata ctcggacttg ggcttggcga   9360

tatcaacctc ggacatggac gagagcgatg tggaagaggc cgagtggcgg ggagagtctg   9420

aaggagagac ggcggcagac tcagaatccg tcacagtagt tgaggtgacg gtgcgtctaa   9480

gcgcagggtt ctgcttgggc agagccgaag tggacgccat ggttgatgtg tgtttaattc   9540

aagaatgaat atagagaaga gaagaagaaa aaagattcaa ttgagccggc gatgcagacc   9600

cttatataaa tgttgccttg gacagacgga gcaagcccgc ccaaacctac gttcggtata   9660

atatgttaag ctttttaaca caaaggtttg gcttggggta acctgatgtg gtgcaaaaga   9720

ccgggcgttg gcgagccatt gcgcgggcga atggggccgt gactcgtctc aaattcgagg   9780

gcgtgcctca attcgtgccc ccgtggcttt ttcccgccgt ttccgccccg tttgcaccac   9840

tgcagccgct tctttggttc ggacaccttg ctgcgagcta ggtgccttgt gctacttaaa   9900

aagtggcctc ccaacaccaa catgacatga gtgcgtgggc caagacacgt tggcggggtc   9960

gcagtcggct caatggcccg gaaaaaacgc tgctggagct ggttcggacg cagtccgccg  10020

cggcgtatgg atatccgcaa ggttccatag cgccattgcc ctccgtcggc gtctatcccg  10080

caacctctaa atagagcggg aatataaccc aagcttcttt tttttccttt aacacgcaca  10140

cccccaacta tcatgttgct gctgctgttt gactctactc tgtggagggg tgctcccacc  10200

caacccaacc tacaggtgga tccggcgctg tgattggctg ataagtctcc tatccggact  10260

aattctgacc aatgggacat gcgcgcagga cccaaatgcc gcaattacgt aaccccaacg  10320

aaatgcctac ccctctttgg agcccagcgg ccccaaatcc ccccaagcag cccggttcta  10380

ccggcttcca tctccaagca caagcagccc ggttctaccg gcttccatct ccaagcaccc  10440

ctttctccac accccacaaa aagacccgtg caggacatcc tactgcgtcg acatcattta  10500

aattccttca cttcaagttc attcttcatc tgcttctgtt ttactttgac aggcaaatga  10560

agacatggta cgacttgatg gaggccaaga acgccatttc accccgagac accgaagtgc  10620

ctgaaatcct ggctgccccc attgataaca tcggaaacta cggtattccg gaaagtgtat  10680

atagaacctt tccccagctt gtgtctgtgg atatggatgg tgtaatcccc tttgagtact  10740

cgtcttggct tctctccgag cagtatgagg ctctctaatc tagcgcattt aatatctcaa  10800

tgtatttata tatttatctt ctcatgcggc cgctcactga atctttttgg ctcccttgtg  10860

cttcctgacg atatacgttt gcacatagaa attcaagaac aaacacaaga ctgtgccaac  10920

ataaaagtaa ttgaagaacc agccaaacat cctcatccca tcttggcgat aacagggaat  10980

gttcctgtac ttccagacaa tgtagaaacc aacattgaat tgaatgatct gcattgatgt  11040

aatcagggat tttggcatgg ggaacttcag cttgatcaat ctggtccaat aataaccgta  11100

catgatccag tggatgaaac cattcaacag cacaaaaatc caaacagctt catttcggta  11160

attatagaac agccacatat ccatcggtgc ccccaaatga tggaagaatt gcaaccaggt  11220

cagaggcttg cccatcagtg gcaaatagaa ggagtcaata tactccagga acttgctcaa  11280

atagaacaac tgcgtggtga tcctgaagac gttgttgtca aaagccttct cgcagttgtc  11340

agacataaca ccgatggtgt acatggcata tgccattgag aggaatgatc ccaacgaata  11400

aatggacatg agaaggttgt aattggtgaa aacaaacttc atacgagact gaccttttgg  11460

accaaggggg ccaagagtga acttcaagat gacaaatgcg atggacaagt aaagcacctc  11520

acagtgactg gcatcactcc agagttgggc ataatcaact ggttgggtaa aacttcctgc  11580

ccaattgaga ctatttcatt caccacctcc atggccattg ctgtagatat gtcttgtgtg  11640

taagggggtt ggggtggttg tttgtgttct tgacttttgt gttagcaagg gaagacgggc  11700

aaaaaagtga gtgtggttgg gagggagaga cgagccttat atataatgct tgtttgtgtt  11760

tgtgcaagtg gacgccgaaa cgggcaggag ccaaactaaa caaggcagac aatgcgagct  11820

taattggatt gcctgatggg caggggttag ggctcgatca atgggggtgc gaagtgacaa  11880

aattgggaat taggttcgca agcaaggctg acaagacttt ggcccaaaca tttgtacgcg  11940

gtggacaaca ggagccaccc atcgtctgtc acgggctagc cggtcgtgcg tcctgtcagg  12000

ctccacctag gctccatgcc actccataca atcccactag tgtaccgcta ggccgctttt  12060

agctcccatc taagaccccc ccaaaacctc cactgtacag tgcactgtac tgtgtggcga  12120

tcaagggcaa gggaaaaaag gcgcaaacat gcacgcatgg aatgacgtag gtaaggcgtt  12180

actagactga aaagtggcac atttcggcgt gccaaagggt cctaggtgcg tttcgcgagc  12240

tgggcgccag gccaagccgc tccaaaacgc ctctccgact ccctccagcg gcctccatat  12300

ccccatccct ctccacagca atgttgttaa gccttgcaaa cgaaaaaata gaaaggctaa  12360

taagcttcca atattgtggt gtacgctgca taacgcaaca atgagcgcca aacaacacac  12420

acacacagca cacagcagca ttaaccacga tgaacagcat gacattacag gtgggtgtgt  12480

aatcagggcc ctgattgctg gtggtgggag cccccatcat gggcagatct gcgtacactg  12540

tttaaacagt gtacgcagat ctactataga ggaacattta aattgccccg gagaagacgg  12600

ccaggccgcc tagatgacaa attcaacaac tcacagctga ctttctgcca ttgccactag  12660

gggggggcct ttttatatgg ccaagccaag ctctccacgt cggttgggct gcacccaaca  12720

ataaatgggt agggttgcac caacaaaggg atgggatggg gggtagaaga tacgaggata  12780

acggggctca atggcacaaa taagaacgaa tactgccatt aagactcgtg atccagcgac  12840

tgacaccatt gcatcatcta agggcctcaa aactacctcg gaactgctgc gctgatctgg  12900

acaccacaga ggttccgagc actttaggtt gcaccaaatg tcccaccagg tgcaggcaga  12960

aaacgctgga acagcgtgta cagtttgtct taacaaaaag tgagggcgct gaggtcgagc  13020

agggtggtgt gacttgttat agcctttaga gctgcgaaag cgcgtatgga tttggctcat  13080

caggccagat tgagggtctg tggacacatg tcatgttagt gtacttcaat cgccccctgg  13140

atatagcccc gacaataggc cgtggcctca tttttttgcc ttccgcacat ttccattgct  13200

cgatacccac accttgcttc tcctgcactt gccaacctta atactggttt acattgacca  13260

acatcttaca agcggggggc ttgtctaggg tatatataaa cagtggctct cccaatcggt  13320

tgccagtctc ttttttcctt tctttcccca cagattcgaa atctaaacta cacatcacag  13380

aattccgagc cgtgagtatc cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat  13440

gacacaatcc gaaagtcgct agcaacacac actctctaca caaactaacc cagctctggt  13500

accatggagg tcgtgaacga aatcgtctcc attggccagg aggttcttcc caaggtcgac  13560

tatgctcagc tctggtctga tgcctcgcac tgcgaggtgc tgtacctctc catcgccttc  13620

gtcatcctga agttcaccct tggtcctctc ggacccaagg gtcagtctcg aatgaagttt  13680

gtgttcacca actacaacct gctcatgtcc atctactcgc tgggctcctt cctctctatg  13740

gcctacgcca tgtacaccat tggtgtcatg tccgacaact gcgagaaggc tttcgacaac  13800

aatgtcttcc gaatcaccac tcagctgttc tacctcagca agttcctcga gtacattgac  13860

tccttctatc tgcccctcat gggcaagcct ctgacctggt tgcagttctt tcaccatctc  13920

ggagctccta tggacatgtg gctgttctac aactaccgaa acgaagccgt ttggatcttt  13980

gtgctgctca acggcttcat tcactggatc atgtacggct actattggac ccgactgatc  14040

aagctcaagt tccctatgcc caagtccctg attacttcta tgcagatcat tcagttcaac  14100

gttggcttct acatcgtctg gaagtaccgg aacattccct gctaccgaca agatggaatg  14160

agaatgtttg gctggttttt caactacttc tacgttggta ctgtcctgtg tctgttcctc  14220

aacttctacg tgcagaccta catcgtccga aagcacaagg gagccaaaaa gattcagtga  14280

gcggccgcat gtacatacaa gattatttat agaaatgaat cgcgatcgaa caaagagtac  14340

gagtgtacga gtaggggatg atgataaaag tggaagaagt tccgcatctt tggatttatc  14400

aacgtgtagg acgatacttc ctgtaaaaat gcaatgtctt taccataggt tctgctgtag  14460

atgttattaa ctaccattaa catgtctact tgtacagttg cagaccagtt ggagtataga  14520

atggtacact taccaaaaag tgttgatggt tgtaactacg atatataaaa ctgttgacgg  14580

gatccccgct gatatgccta aggaacaatc aaagaggaag atattaattc agaatgctag  14640

tatacagtta gggat                                                   14655


<210>  18
<211>  34
<212>  DNA
<213>  Escherichia coli

<400>  18
ataacttcgt ataatgtatg ctatacgaag ttat                                 34


<210>  19
<211>  828
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  misc_feature
<223>  synthetic C16/18 elongase codon-optimized for Yarrowia 
       lipolytica; U.S. Patent Application No. 11/253882, filed October 
       19, 2005

<400>  19
atggagtctg gacccatgcc tgctggcatt cccttccctg agtactatga cttctttatg     60

gactggaaga ctcccctggc catcgctgcc acctacactg ctgccgtcgg tctcttcaac    120

cccaaggttg gcaaggtctc ccgagtggtt gccaagtcgg ctaacgcaaa gcctgccgag    180

cgaacccagt ccggagctgc catgactgcc ttcgtctttg tgcacaacct cattctgtgt    240

gtctactctg gcatcacctt ctactacatg tttcctgcta tggtcaagaa cttccgaacc    300

cacacactgc acgaagccta ctgcgacacg gatcagtccc tctggaacaa cgcacttggc    360

tactggggtt acctcttcta cctgtccaag ttctacgagg tcattgacac catcatcatc    420

atcctgaagg gacgacggtc ctcgctgctt cagacctacc accatgctgg agccatgatt    480

accatgtggt ctggcatcaa ctaccaagcc actcccattt ggatctttgt ggtcttcaac    540

tccttcattc acaccatcat gtactgttac tatgccttca cctctatcgg attccatcct    600

cctggcaaaa agtacctgac ttcgatgcag attactcagt ttctggtcgg tatcaccatt    660

gccgtgtcct acctcttcgt tcctggctgc atccgaacac ccggtgctca gatggctgtc    720

tggatcaacg tcggctacct gtttcccttg acctatctgt tcgtggactt tgccaagcga    780

acctactcca agcgatctgc cattgccgct cagaaaaagg ctcagtaa                 828


<210>  20
<211>  8910
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pFmD8S

<400>  20
catggtgaag tccaagcgac aggctctgcc cctcaccatc gacggaacta cctacgacgt     60

ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact accagggacg    120

agatgctact gacgccttca tggttatgca ctctcaggaa gccttcgaca agctcaagcg    180

aatgcccaag atcaacccct cctccgagct gcctccccag gctgccgtca acgaagctca    240

ggaggatttc cgaaagctcc gagaagagct gatcgccact ggcatgtttg acgcctctcc    300

cctctggtac tcgtacaaga tctccaccac cctgggtctt ggcgtgcttg gatacttcct    360

gatggtccag taccagatgt acttcattgg tgctgtgctg ctcggtatgc actaccagca    420

aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc gaaactggaa    480

taacctcgtg ggtctggtct ttggcaacgg actccagggc ttctccgtga cctggtggaa    540

ggacagacac aacgcccatc attctgctac caacgttcag ggtcacgatc ccgacattga    600

taacctgcct ctgctcgcct ggtccgagga cgatgtcact cgagcttctc ccatctcccg    660

aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat    720

ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta    780

ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc tcaagaccct    840

gttccacctc ttctttatgc cctccatcct gacctcgctc ctggtgttct ttgtttccga    900

gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg aaccactacc ctctggagaa    960

gatcggtgat tccgtctggg acggacatgg cttctctgtg ggtcagatcc atgagaccat   1020

gaacattcga cgaggcatca ttactgactg gttctttgga ggcctgaact accagatcga   1080

gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga   1140

acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg aaggtctcgt   1200

catcctgctc cgatacctgg ccgtgttcgc tcgaatggcc gagaagcagc ccgctggcaa   1260

ggctctctaa gcggccgcca ccgcggcccg agattccggc ctcttcggcc gccaagcgac   1320

ccgggtggac gtctagaggt acctagcaat taacagatag tttgccggtg ataattctct   1380

taacctccca cactcctttg acataacgat ttatgtaacg aaactgaaat ttgaccagat   1440

attgtgtccg cggtggagct ccagcttttg ttccctttag tgagggttaa tttcgagctt   1500

ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca   1560

caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact   1620

cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct   1680

gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc   1740

ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca   1800

ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg   1860

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca   1920

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa   1980

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc   2040

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc   2100

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct   2160

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg   2220

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag   2280

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta   2340

cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg   2400

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt   2460

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt   2520

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag   2580

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat   2640

ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc   2700

tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat   2760

aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc   2820

acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag   2880

aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag   2940

agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt   3000

ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg   3060

agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt   3120

tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc   3180

tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc   3240

attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa   3300

taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg   3360

aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc   3420

caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag   3480

gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt   3540

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt   3600

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc   3660

acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt   3720

gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct   3780

cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg   3840

atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag   3900

tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa   3960

tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga   4020

tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa   4080

atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg   4140

cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa   4200

gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt   4260

tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg   4320

ccccccctcg aggtcgatgg tgtcgataag cttgatatcg aattcatgtc acacaaaccg   4380

atcttcgcct caaggaaacc taattctaca tccgagagac tgccgagatc cagtctacac   4440

tgattaattt tcgggccaat aatttaaaaa aatcgtgtta tataatatta tatgtattat   4500

atatatacat catgatgata ctgacagtca tgtcccattg ctaaatagac agactccatc   4560

tgccgcctcc aactgatgtt ctcaatattt aaggggtcat ctcgcattgt ttaataataa   4620

acagactcca tctaccgcct ccaaatgatg ttctcaaaat atattgtatg aacttatttt   4680

tattacttag tattattaga caacttactt gctttatgaa aaacacttcc tatttaggaa   4740

acaatttata atggcagttc gttcatttaa caatttatgt agaataaatg ttataaatgc   4800

gtatgggaaa tcttaaatat ggatagcata aatgatatct gcattgccta attcgaaatc   4860

aacagcaacg aaaaaaatcc cttgtacaac ataaatagtc atcgagaaat atcaactatc   4920

aaagaacagc tattcacacg ttactattga gattattatt ggacgagaat cacacactca   4980

actgtctttc tctcttctag aaatacaggt acaagtatgt actattctca ttgttcatac   5040

ttctagtcat ttcatcccac atattccttg gatttctctc caatgaatga cattctatct   5100

tgcaaattca acaattataa taagatatac caaagtagcg gtatagtggc aatcaaaaag   5160

cttctctggt gtgcttctcg tatttatttt tattctaatg atccattaaa ggtatatatt   5220

tatttcttgt tatataatcc ttttgtttat tacatgggct ggatacataa aggtattttg   5280

atttaatttt ttgcttaaat tcaatccccc ctcgttcagt gtcaactgta atggtaggaa   5340

attaccatac ttttgaagaa gcaaaaaaaa tgaaagaaaa aaaaaatcgt atttccaggt   5400

tagacgttcc gcagaatcta gaatgcggta tgcggtacat tgttcttcga acgtaaaagt   5460

tgcgctccct gagatattgt acatttttgc ttttacaagt acaagtacat cgtacaacta   5520

tgtactactg ttgatgcatc cacaacagtt tgttttgttt ttttttgttt tttttttttc   5580

taatgattca ttaccgctat gtatacctac ttgtacttgt agtaagccgg gttattggcg   5640

ttcaattaat catagactta tgaatctgca cggtgtgcgc tgcgagttac ttttagctta   5700

tgcatgctac ttgggtgtaa tattgggatc tgttcggaaa tcaacggatg ctcaaccgat   5760

ttcgacagta ataatttgaa tcgaatcgga gcctaaaatg aacccgagta tatctcataa   5820

aattctcggt gagaggtctg tgactgtcag tacaaggtgc cttcattatg ccctcaacct   5880

taccatacct cactgaatgt agtgtacctc taaaaatgaa atacagtgcc aaaagccaag   5940

gcactgagct cgtctaacgg acttgatata caaccaatta aaacaaatga aaagaaatac   6000

agttctttgt atcatttgta acaattaccc tgtacaaact aaggtattga aatcccacaa   6060

tattcccaaa gtccacccct ttccaaattg tcatgcctac aactcatata ccaagcacta   6120

acctaccaaa caccactaaa accccacaaa atatatctta ccgaatatac agtaacaagc   6180

taccaccaca ctcgttgggt gcagtcgcca gcttaaagat atctatccac atcagccaca   6240

actcccttcc tttaataaac cgactacacc cttggctatt gaggttatga gtgaatatac   6300

tgtagacaag acactttcaa gaagactgtt tccaaaacgt accactgtcc tccactacaa   6360

acacacccaa tctgcttctt ctagtcaagg ttgctacacc ggtaaattat aaatcatcat   6420

ttcattagca gggcagggcc ctttttatag agtcttatac actagcggac cctgccggta   6480

gaccaacccg caggcgcgtc agtttgctcc ttccatcaat gcgtcgtaga aacgacttac   6540

tccttcttga gcagctcctt gaccttgttg gcaacaagtc tccgacctcg gaggtggagg   6600

aagagcctcc gatatcggcg gtagtgatac cagcctcgac ggactccttg acggcagcct   6660

caacagcgtc accggcgggc ttcatgttaa gagagaactt gagcatcatg gcggcagaca   6720

gaatggtggc aatggggttg accttctgct tgccgagatc gggggcagat ccgtgacagg   6780

gctcgtacag accgaacgcc tcgttggtgt cgggcagaga agccagagag gcggagggca   6840

gcagacccag agaaccgggg atgacggagg cctcgtcgga gatgatatcg ccaaacatgt   6900

tggtggtgat gatgatacca ttcatcttgg agggctgctt gatgaggatc atggcggccg   6960

agtcgatcag ctggtggttg agctcgagct gggggaattc gtccttgagg actcgagtga   7020

cagtctttcg ccaaagtcga gaggaggcca gcacgttggc cttgtcaaga gaccacacgg   7080

gaagaggggg gttgtgctga agggccagga aggcggccat tcgggcaatt cgctcaacct   7140

caggaacgga gtaggtctcg gtgtcggaag cgacgccaga tccgtcatcc tcctttcgct   7200

ctccaaagta gatacctccg acgagctctc ggacaatgat gaagtcggtg ccctcaacgt   7260

ttcggatggg ggagagatcg gcgagcttgg gcgacagcag ctggcagggt cgcaggttgg   7320

cgtacaggtt caggtccttt cgcagcttga ggagaccctg ctcgggtcgc acgtcggttc   7380

gtccgtcggg agtggtccat acggtgttgg cagcgcctcc gacagcaccg agcataatag   7440

agtcagcctt tcggcagatg tcgagagtag cgtcggtgat gggctcgccc tccttctcaa   7500

tggcagctcc tccaatgagt cggtcctcaa acacaaactc ggtgccggag gcctcagcaa   7560

cagacttgag caccttgacg gcctcggcaa tcacctcggg gccacagaag tcgccgccga   7620

gaagaacaat cttcttggag tcagtcttgg tcttcttagt ttcgggttcc attgtggatg   7680

tgtgtggttg tatgtgtgat gtggtgtgtg gagtgaaaat ctgtggctgg caaacgctct   7740

tgtatatata cgcacttttg cccgtgctat gtggaagact aaacctccga agattgtgac   7800

tcaggtagtg cggtatcggc tagggaccca aaccttgtcg atgccgatag cgctatcgaa   7860

cgtaccccag ccggccggga gtatgtcgga ggggacatac gagatcgtca agggtttgtg   7920

gccaactggt aaataaatga tgtcgacgtt taaacagtgt acgcagatct actatagagg   7980

aacatttaaa ttgccccgga gaagacggcc aggccgccta gatgacaaat tcaacaactc   8040

acagctgact ttctgccatt gccactaggg gggggccttt ttatatggcc aagccaagct   8100

ctccacgtcg gttgggctgc acccaacaat aaatgggtag ggttgcacca acaaagggat   8160

gggatggggg gtagaagata cgaggataac ggggctcaat ggcacaaata agaacgaata   8220

ctgccattaa gactcgtgat ccagcgactg acaccattgc atcatctaag ggcctcaaaa   8280

ctacctcgga actgctgcgc tgatctggac accacagagg ttccgagcac tttaggttgc   8340

accaaatgtc ccaccaggtg caggcagaaa acgctggaac agcgtgtaca gtttgtctta   8400

acaaaaagtg agggcgctga ggtcgagcag ggtggtgtga cttgttatag cctttagagc   8460

tgcgaaagcg cgtatggatt tggctcatca ggccagattg agggtctgtg gacacatgtc   8520

atgttagtgt acttcaatcg ccccctggat atagccccga caataggccg tggcctcatt   8580

tttttgcctt ccgcacattt ccattgctcg gtacccacac cttgcttctc ctgcacttgc   8640

caaccttaat actggtttac attgaccaac atcttacaag cggggggctt gtctagggta   8700

tatataaaca gtggctctcc caatcggttg ccagtctctt ttttcctttc tttccccaca   8760

gattcgaaat ctaaactaca catcacagaa ttccgagccg tgagtatcca cgacaagatc   8820

agtgtcgaga cgacgcgttt tgtgtaatga cacaatccga aagtcgctag caacacacac   8880

tctctacaca aactaaccca gctctggtac                                    8910


<210>  21
<211>  7145
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKUNFmkF2


<220>
<221>  misc_feature
<222>  (692)..(695)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (700)..(700)
<223>  n is a, c, g, or t

<400>  21
catggcgtcc acttcggctc tgcccaagca gaaccctgcg cttagacgca ccgtcacctc     60

aactactgtg acggattctg agtctgccgc cgtctctcct tcagactctc cccgccactc    120

ggcctcttcc acatcgctct cgtccatgtc cgaggttgat atcgccaagc ccaagtccga    180

gtatggtgtc atgctcgaca cctacggcaa ccagttcgag gttcccgact ttaccatcaa    240

ggacatctac aatgccatcc ctaagcactg cttcaagcgc tccgctctca agggatacgg    300

ttatatcctc cgcgacattg tcctcctgac taccactttc agcatctggt acaactttgt    360

gacccccgaa tatatcccct ccacccccgc ccgcgctggt ctgtgggccg tgtacaccgt    420

tcttcagggt cttttcggta ctggtctctg ggttattgcc catgagtgcg gtcacggtgc    480

tttctccgat tctcgcatca tcaacgacat tactggctgg gttcttcact cttccctcct    540

tgtcccctac ttcagctggc aaatctccca ccgaaagcac cacaaggcca ctggcaacat    600

ggagcgtgac atggtcttcg ttccccgaac ccgcgagcag caggctactc gtctcggaaa    660

gatgacccac gagctcgctc atcttactga gnnnntcgtn ggctggccca actacctcat    720

caccaatgtt accggccaca actaccacga gcgccagcgt gagggtcgcg gcaagggcaa    780

gcataacggc ctcggcggtg gtgttaacca cttcgatccc cgcagccctc tgtacgagaa    840

cagtgacgct aagctcatcg tcctcagcga tattggtatc ggtctgatgg ccactgctct    900

gtacttcctc gttcagaagt tcggtttcta caacatggcc atctggtact ttgttcccta    960

cctctgggtt aaccactggc tcgttgccat caccttcctc cagcacaccg accctaccct   1020

tccccactac accaacgacg agtggaactt cgtccgtggt gccgctgcta ccattgaccg   1080

tgagatgggc ttcatcggcc gccaccttct ccacggcatc atcgagactc atgtcctcca   1140

ccactacgtc agcagcatcc ccttctacaa cgcggacgag gccaccgagg ccattaagcc   1200

catcatgggc aagcactacc gggctgatgt ccaggatggt cctcgtggct tcatccgcgc   1260

catgtaccgc agtgcgcgta tgtgccagtg ggttgagccc agcgctggtg ccgagggtgc   1320

tggtaagggt gttctgttct tccgcaaccg caacaacgtg ggcacccccc ccgctgttat   1380

caagcccgtt gcttaagtag gcgcggccgc tatttatcac tctttacaac ttctacctca   1440

actatctact ttaataaatg aatatcgttt attctctatg attactgtat atgcgttcct   1500

ctaagacaaa tcgaaaccag catgtgatcg aatggcatac aaaagtttct tccgaagttg   1560

atcaatgtcc tgatagtcag gcagcttgag aagattgaca caggtggagg ccgtagggaa   1620

ccgatcaacc tgtctaccag cgttacgaat ggcaaatgac gggttcaaag ccttgaatcc   1680

ttgcaatggt gccttggata ctgatgtcac aaacttaaga agcagccgct tgtcctcttc   1740

ctcgatcgat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac   1800

aacgtacgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca taaggtccga   1860

ccttatcggc aagctcaatg agctccttgg tggtggtaac atccagagaa gcacacaggt   1920

tggttttctt ggctgccacg agcttgagca ctcgagcggc aaaggcggac ttgtggacgt   1980

tagctcgagc ttcgtaggag ggcattttgg tggtgaagag gagactgaaa taaatttagt   2040

ctgcagaact ttttatcgga accttatctg gggcagtgaa gtatatgtta tggtaatagt   2100

tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc aaattagaaa   2160

gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca tgatgaaagc   2220

cagcaatgac gttgcagctg atattgttgt cggccaaccg cgccgaaaac gcagctgtca   2280

gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac tcatagttgg   2340

agtcgtactc caaaggcggc aatgacgagt cagacagata ctcgtcgacc ttttccttgg   2400

gaaccaccac cgtcagccct tctgactcac gtattgtagc caccgacaca ggcaacagtc   2460

cgtggatagc agaatatgtc ttgtcggtcc atttctcacc aactttaggc gtcaagtgaa   2520

tgttgcagaa gaagtatgtg ccttcattga gaatcggtgt tgctgatttc aataaagtct   2580

tgagatcagt ttggcgcgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg   2640

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg   2700

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg   2760

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa   2820

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg   2880

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc   2940

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc   3000

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc   3060

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg   3120

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc   3180

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga   3240

gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc   3300

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac   3360

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg   3420

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc   3480

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa   3540

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta   3600

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt   3660

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag   3720

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca   3780

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc   3840

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt   3900

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag   3960

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt   4020

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat   4080

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt   4140

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc   4200

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat   4260

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag   4320

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt   4380

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg   4440

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta   4500

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc   4560

gcgcacattt ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa   4620

ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa   4680

tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa   4740

atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact   4800

attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc   4860

actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa   4920

tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc   4980

gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt   5040

cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat   5100

tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta   5160

cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt   5220

tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc   5280

gaattgggcc cgacgtcgca tgcagtggtg gtattgtgac tggggatgta gttgagaata   5340

agtcatacac aagtcagctt tcttcgagcc tcatataagt ataagtagtt caacgtatta   5400

gcactgtacc cagcatctcc gtatcgagaa acacaacaac atgccccatt ggacagatca   5460

tgcggataca caggttgtgc agtatcatac atactcgatc agacaggtcg tctgaccatc   5520

atacaagctg aacaagcgct ccatacttgc acgctctcta tatacacagt taaattacat   5580

atccatagtc taacctctaa cagttaatct tctggtaagc ctcccagcca gccttctggt   5640

atcgcttggc ctcctcaata ggatctcggt tctggccgta cagacctcgg ccgacaatta   5700

tgatatccgt tccggtagac atgacatcct caacagttcg gtactgctgt ccgagagcgt   5760

ctcccttgtc gtcaagaccc accccggggg tcagaataag ccagtcctca gagtcgccct   5820

taattaattt gaatcgaatc gatgagccta aaatgaaccc gagtatatct cataaaattc   5880

tcggtgagag gtctgtgact gtcagtacaa ggtgccttca ttatgccctc aaccttacca   5940

tacctcactg aatgtagtgt acctctaaaa atgaaataca gtgccaaaag ccaaggcact   6000

gagctcgtct aacggacttg atatacaacc aattaaaaca aatgaaaaga aatacagttc   6060

tttgtatcat ttgtaacaat taccctgtac aaactaaggt attgaaatcc cacaatattc   6120

ccaaagtcca cccctttcca aattgtcatg cctacaactc atataccaag cactaaccta   6180

ccgtttaaac agtgtacgca gatctactat agaggaacat ttaaattgcc ccggagaaga   6240

cggccaggcc gcctagatga caaattcaac aactcacagc tgactttctg ccattgccac   6300

tagggggggg cctttttata tggccaagcc aagctctcca cgtcggttgg gctgcaccca   6360

acaataaatg ggtagggttg caccaacaaa gggatgggat ggggggtaga agatacgagg   6420

ataacggggc tcaatggcac aaataagaac gaatactgcc attaagactc gtgatccagc   6480

gactgacacc attgcatcat ctaagggcct caaaactacc tcggaactgc tgcgctgatc   6540

tggacaccac agaggttccg agcactttag gttgcaccaa atgtcccacc aggtgcaggc   6600

agaaaacgct ggaacagcgt gtacagtttg tcttaacaaa aagtgagggc gctgaggtcg   6660

agcagggtgg tgtgacttgt tatagccttt agagctgcga aagcgcgtat ggatttggct   6720

catcaggcca gattgagggt ctgtggacac atgtcatgtt agtgtacttc aatcgccccc   6780

tggatatagc cccgacaata ggccgtggcc tcattttttt gccttccgca catttccatt   6840

gctcgatacc cacaccttgc ttctcctgca cttgccaacc ttaatactgg tttacattga   6900

ccaacatctt acaagcgggg ggcttgtcta gggtatatat aaacagtggc tctcccaatc   6960

ggttgccagt ctcttttttc ctttctttcc ccacagattc gaaatctaaa ctacacatca   7020

cagaattccg agccgtgagt atccacgaca agatcagtgt cgagacgacg cgttttgtgt   7080

aatgacacaa tccgaaagtc gctagcaaca cacactctct acacaaacta acccagctct   7140

ggtac                                                               7145


<210>  22
<211>  5473
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW287F

<400>  22
ggccgcattg atgattggaa acacacacat gggttatatc taggtgagag ttagttggac     60

agttatatat taaatcagct atgccaacgg taacttcatt catgtcaacg aggaaccagt    120

gactgcaagt aatatagaat ttgaccacct tgccattctc ttgcactcct ttactatatc    180

tcatttattt cttatataca aatcacttct tcttcccagc atcgagctcg gaaacctcat    240

gagcaataac atcgtggatc tcgtcaatag agggcttttt ggactccttg ctgttggcca    300

ccttgtcctt gctgtctggc tcattctgtt tcaacgcctt ttaattaatc gagcttggcg    360

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac    420

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca    480

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat    540

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc    600

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca    660

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca    720

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg    780

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg    840

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt    900

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt    960

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc   1020

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt   1080

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt   1140

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc   1200

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa   1260

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt   1320

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct   1380

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta   1440

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa   1500

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc   1560

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact   1620

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc   1680

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt   1740

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta   1800

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg   1860

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt   1920

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc   1980

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt   2040

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc   2100

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc   2160

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa   2220

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac   2280

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa   2340

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt   2400

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa   2460

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct   2520

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc   2580

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc   2640

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt   2700

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg   2760

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt   2820

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta   2880

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt   2940

aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca   3000

actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg   3060

gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta   3120

aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc   3180

ccctcgaggt cgacgtttaa acagtgtacg cagtactata gaggaacatc gattgccccg   3240

gagaagacgg ccaggccgcc tagatgacaa attcaacaac tcacagctga ctttctgcca   3300

ttgccactag gggggggcct ttttatatgg ccaagccaag ctctccacgt cggttgggct   3360

gcacccaaca ataaatgggt agggttgcac caacaaaggg atgggatggg gggtagaaga   3420

tacgaggata acggggctca atggcacaaa taagaacgaa tactgccatt aagactcgtg   3480

atccagcgac tgacaccatt gcatcatcta agggcctcaa aactacctcg gaactgctgc   3540

gctgatctgg acaccacaga ggttccgagc actttaggtt gcaccaaatg tcccaccagg   3600

tgcaggcaga aaacgctgga acagcgtgta cagtttgtct taacaaaaag tgagggcgct   3660

gaggtcgagc agggtggtgt gacttgttat agcctttaga gctgcgaaag cgcgtatgga   3720

tttggctcat caggccagat tgagggtctg tggacacatg tcatgttagt gtacttcaat   3780

cgccccctgg atatagcccc gacaataggc cgtggcctca tttttttgcc ttccgcacat   3840

ttccattgct cggtacccac accttgcttc tcctgcactt gccaacctta atactggttt   3900

acattgacca acatcttaca agcggggggc ttgtctaggg tatatataaa cagtggctct   3960

cccaatcggt tgccagtctc ttttttcctt tctttcccca cagattcgaa atctaaacta   4020

cacatcacac aatgcctgtt actgacgtcc ttaagcgaaa gtccggtgtc atcgtcggcg   4080

acgatgtccg agccgtgagt atccacgaca agatcagtgt cgagacgacg cgttttgtgt   4140

aatgacacaa tccgaaagtc gctagcaaca cacactctct acacaaacta acccagctct   4200

ccatggtgaa gtccaagcga caggctctgc ccctcaccat cgacggaact acctacgacg   4260

tctccgcttg ggtgaacttc caccctggtg gagctgaaat cattgagaac taccagggac   4320

gagatgctac tgacgccttc atggttatgc actctcagga agccttcgac aagctcaagc   4380

gaatgcccaa gatcaacccc tcctccgagc tgcctcccca ggctgccgtc aacgaagctc   4440

aggaggattt ccgaaagctc cgagaagagc tgatcgccac tggcatgttt gacgcctctc   4500

ccctctggta ctcgtacaag atctccacca ccctgggtct tggcgtgctt ggatacttcc   4560

tgatggtcca gtaccagatg tacttcattg gtgctgtgct gctcggtatg cactaccagc   4620

aaatgggatg gctgtctcat gacatctgcc accaccagac cttcaagaac cgaaactgga   4680

ataacctcgt gggtctggtc tttggcaacg gactccaggg cttctccgtg acctggtgga   4740

aggacagaca caacgcccat cattctgcta ccaacgttca gggtcacgat cccgacattg   4800

ataacctgcc tctgctcgcc tggtccgagg acgatgtcac tcgagcttct cccatctccc   4860

gaaagctcat tcagttccaa cagtactatt tcctggtcat ctgtattctc ctgcgattca   4920

tctggtgttt ccagtctgtg ctgaccgttc gatccctcaa ggaccgagac aaccagttct   4980

accgatctca gtacaagaaa gaggccattg gactcgctct gcactggact ctcaagaccc   5040

tgttccacct cttctttatg ccctccatcc tgacctcgct cctggtgttc tttgtttccg   5100

agctcgtcgg tggcttcgga attgccatcg tggtcttcat gaaccactac cctctggaga   5160

agatcggtga ttccgtctgg gacggacatg gcttctctgt gggtcagatc catgagacca   5220

tgaacattcg acgaggcatc attactgact ggttctttgg aggcctgaac taccagatcg   5280

agcaccatct ctggcccacc ctgcctcgac acaacctcac tgccgtttcc taccaggtgg   5340

aacagctgtg ccagaagcac aacctcccct accgaaaccc tctgccccat gaaggtctcg   5400

tcatcctgct ccgatacctg gccgtgttcg ctcgaatggc cgagaagcag cccgctggca   5460

aggctctcta agc                                                      5473


<210>  23
<211>  9513
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW214

<400>  23
ggtggagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat     60

ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag    120

ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg    180

cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa    240

tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca    300

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg    360

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc    420

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc    480

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac    540

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc    600

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata    660

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc    720

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca    780

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag    840

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta    900

gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg    960

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc   1020

agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt   1080

ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa   1140

ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat   1200

atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga   1260

tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac   1320

gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg   1380

ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg   1440

caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt   1500

cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct   1560

cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat   1620

cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta   1680

agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca   1740

tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat   1800

agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac   1860

atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa   1920

ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt   1980

cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg   2040

caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat   2100

attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt   2160

agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc   2220

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac   2280

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg   2340

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt   2400

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc   2460

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct   2520

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga   2580

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga   2640

attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg   2700

ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc   2760

tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac   2820

ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga   2880

ggtcgatggt gtcgataagc ttgatatcga attcatgtca cacaaaccga tcttcgcctc   2940

aaggaaacct aattctacat ccgagagact gccgagatcc agtctacact gattaatttt   3000

cgggccaata atttaaaaaa atcgtgttat ataatattat atgtattata tatatacatc   3060

atgatgatac tgacagtcat gtcccattgc taaatagaca gactccatct gccgcctcca   3120

actgatgttc tcaatattta aggggtcatc tcgcattgtt taataataaa cagactccat   3180

ctaccgcctc caaatgatgt tctcaaaata tattgtatga acttattttt attacttagt   3240

attattagac aacttacttg ctttatgaaa aacacttcct atttaggaaa caatttataa   3300

tggcagttcg ttcatttaac aatttatgta gaataaatgt tataaatgcg tatgggaaat   3360

cttaaatatg gatagcataa atgatatctg cattgcctaa ttcgaaatca acagcaacga   3420

aaaaaatccc ttgtacaaca taaatagtca tcgagaaata tcaactatca aagaacagct   3480

attcacacgt tactattgag attattattg gacgagaatc acacactcaa ctgtctttct   3540

ctcttctaga aatacaggta caagtatgta ctattctcat tgttcatact tctagtcatt   3600

tcatcccaca tattccttgg atttctctcc aatgaatgac attctatctt gcaaattcaa   3660

caattataat aagatatacc aaagtagcgg tatagtggca atcaaaaagc ttctctggtg   3720

tgcttctcgt atttattttt attctaatga tccattaaag gtatatattt atttcttgtt   3780

atataatcct tttgtttatt acatgggctg gatacataaa ggtattttga tttaattttt   3840

tgcttaaatt caatcccccc tcgttcagtg tcaactgtaa tggtaggaaa ttaccatact   3900

tttgaagaag caaaaaaaat gaaagaaaaa aaaaatcgta tttccaggtt agacgttccg   3960

cagaatctag aatgcggtat gcggtacatt gttcttcgaa cgtaaaagtt gcgctccctg   4020

agatattgta catttttgct tttacaagta caagtacatc gtacaactat gtactactgt   4080

tgatgcatcc acaacagttt gttttgtttt tttttgtttt ttttttttct aatgattcat   4140

taccgctatg tatacctact tgtacttgta gtaagccggg ttattggcgt tcaattaatc   4200

atagacttat gaatctgcac ggtgtgcgct gcgagttact tttagcttat gcatgctact   4260

tgggtgtaat attgggatct gttcggaaat caacggatgc tcaaccgatt tcgacagtaa   4320

taatttgaat cgaatcggag cctaaaatga acccgagtat atctcataaa attctcggtg   4380

agaggtctgt gactgtcagt acaaggtgcc ttcattatgc cctcaacctt accatacctc   4440

actgaatgta gtgtacctct aaaaatgaaa tacagtgcca aaagccaagg cactgagctc   4500

gtctaacgga cttgatatac aaccaattaa aacaaatgaa aagaaataca gttctttgta   4560

tcatttgtaa caattaccct gtacaaacta aggtattgaa atcccacaat attcccaaag   4620

tccacccctt tccaaattgt catgcctaca actcatatac caagcactaa cctaccaaac   4680

accactaaaa ccccacaaaa tatatcttac cgaatataca gtaacaagct accaccacac   4740

tcgttgggtg cagtcgccag cttaaagata tctatccaca tcagccacaa ctcccttcct   4800

ttaataaacc gactacaccc ttggctattg aggttatgag tgaatatact gtagacaaga   4860

cactttcaag aagactgttt ccaaaacgta ccactgtcct ccactacaaa cacacccaat   4920

ctgcttcttc tagtcaaggt tgctacaccg gtaaattata aatcatcatt tcattagcag   4980

ggcagggccc tttttataga gtcttataca ctagcggacc ctgccggtag accaacccgc   5040

aggcgcgtca gtttgctcct tccatcaatg cgtcgtagaa acgacttact ccttcttgag   5100

cagctccttg accttgttgg caacaagtct ccgacctcgg aggtggagga agagcctccg   5160

atatcggcgg tagtgatacc agcctcgacg gactccttga cggcagcctc aacagcgtca   5220

ccggcgggct tcatgttaag agagaacttg agcatcatgg cggcagacag aatggtggca   5280

atggggttga ccttctgctt gccgagatcg ggggcagatc cgtgacaggg ctcgtacaga   5340

ccgaacgcct cgttggtgtc gggcagagaa gccagagagg cggagggcag cagacccaga   5400

gaaccgggga tgacggaggc ctcgtcggag atgatatcgc caaacatgtt ggtggtgatg   5460

atgataccat tcatcttgga gggctgcttg atgaggatca tggcggccga gtcgatcagc   5520

tggtggttga gctcgagctg ggggaattcg tccttgagga ctcgagtgac agtctttcgc   5580

caaagtcgag aggaggccag cacgttggcc ttgtcaagag accacacggg aagagggggg   5640

ttgtgctgaa gggccaggaa ggcggccatt cgggcaattc gctcaacctc aggaacggag   5700

taggtctcgg tgtcggaagc gacgccagat ccgtcatcct cctttcgctc tccaaagtag   5760

atacctccga cgagctctcg gacaatgatg aagtcggtgc cctcaacgtt tcggatgggg   5820

gagagatcgg cgagcttggg cgacagcagc tggcagggtc gcaggttggc gtacaggttc   5880

aggtcctttc gcagcttgag gagaccctgc tcgggtcgca cgtcggttcg tccgtcggga   5940

gtggtccata cggtgttggc agcgcctccg acagcaccga gcataataga gtcagccttt   6000

cggcagatgt cgagagtagc gtcggtgatg ggctcgccct ccttctcaat ggcagctcct   6060

ccaatgagtc ggtcctcaaa cacaaactcg gtgccggagg cctcagcaac agacttgagc   6120

accttgacgg cctcggcaat cacctcgggg ccacagaagt cgccgccgag aagaacaatc   6180

ttcttggagt cagtcttggt cttcttagtt tcgggttcca ttgtggatgt gtgtggttgt   6240

atgtgtgatg tggtgtgtgg agtgaaaatc tgtggctggc aaacgctctt gtatatatac   6300

gcacttttgc ccgtgctatg tggaagacta aacctccgaa gattgtgact caggtagtgc   6360

ggtatcggct agggacccaa accttgtcga tgccgatagc gctatcgaac gtaccccagc   6420

cggccgggag tatgtcggag gggacatacg agatcgtcaa gggtttgtgg ccaactggta   6480

aataaatgat gtcgacgttt aaacagtgta cgcagtacta tagaggaaca attgccccgg   6540

agaagacggc caggccgcct agatgacaaa ttcaacaact cacagctgac tttctgccat   6600

tgccactagg ggggggcctt tttatatggc caagccaagc tctccacgtc ggttgggctg   6660

cacccaacaa taaatgggta gggttgcacc aacaaaggga tgggatgggg ggtagaagat   6720

acgaggataa cggggctcaa tggcacaaat aagaacgaat actgccatta agactcgtga   6780

tccagcgact gacaccattg catcatctaa gggcctcaaa actacctcgg aactgctgcg   6840

ctgatctgga caccacagag gttccgagca ctttaggttg caccaaatgt cccaccaggt   6900

gcaggcagaa aacgctggaa cagcgtgtac agtttgtctt aacaaaaagt gagggcgctg   6960

aggtcgagca gggtggtgtg acttgttata gcctttagag ctgcgaaagc gcgtatggat   7020

ttggctcatc aggccagatt gagggtctgt ggacacatgt catgttagtg tacttcaatc   7080

gccccctgga tatagccccg acaataggcc gtggcctcat ttttttgcct tccgcacatt   7140

tccattgctc ggtacccaca ccttgcttct cctgcacttg ccaaccttaa tactggttta   7200

cattgaccaa catcttacaa gcggggggct tgtctagggt atatataaac agtggctctc   7260

ccaatcggtt gccagtctct tttttccttt ctttccccac agattcgaaa tctaaactac   7320

acatcacaca atgcctgtta ctgacgtcct taagcgaaag tccggtgtca tcgtcggcga   7380

cgatgtccga gccgtgagta tccacgacaa gatcagtgtc gagacgacgc gttttgtgta   7440

atgacacaat ccgaaagtcg ctagcaacac acactctcta cacaaactaa cccagctctc   7500

catggcatgg atggtacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg   7560

cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag   7620

cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga   7680

tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa   7740

aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt   7800

gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga   7860

tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga   7920

actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa   7980

gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg taatgctcta   8040

caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg   8100

taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg   8160

tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt   8220

gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa   8280

aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa   8340

gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga   8400

agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt   8460

aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat   8520

gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt   8580

taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga   8640

agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc   8700

gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg   8760

tccgcaagtg cacgggaata tttcgccact ggcggaagca acgcgtaaac tcgacccgac   8820

gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga   8880

tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt   8940

ggaaacggca gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca   9000

gccgattatc atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac   9060

cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga   9120

tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca   9180

aggcatattg cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa   9240

gtcggcggct tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca   9300

gcagggaggc aaacaatgat taattaacta gagcggccgc caccgcggcc cgagattccg   9360

gcctcttcgg ccgccaagcg acccgggtgg acgtctagag gtacctagca attaacagat   9420

agtttgccgg tgataattct cttaacctcc cacactcctt tgacataacg atttatgtaa   9480

cgaaactgaa atttgaccag atattgtgtc cgc                                9513


<210>  24
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 1A

<400>  24
tggtaccatg gtgaaggctt ctcgacaggc tctgcccct                            39


<210>  25
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 1B

<400>  25
aggggcagag cctgtcgaga agccttcacc atggtacca                            39


<210>  26
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2A

<400>  26
caggctctgc ccctcgtcat cgacggaact ac                                   32


<210>  27
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2B

<400>  27
gtagttccgt cgatgacgag gggcagagcc tg                                   32


<210>  28
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3A

<400>  28
ctcaccatcg acggaaaggt gtacgacgtc tccgctt                              37


<210>  29
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3B

<400>  29
aagcggagac gtcgtacacc tttccgtcga tggtgag                              37


<210>  30
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 4A

<400>  30
acgtctccgc ttgggtggac gagcaccctg gtggagctg                            39


<210>  31
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 4B

<400>  31
cagctccacc agggtgctcg tccacccaag cggagacgt                            39


<210>  32
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5A

<400>  32
cttccaccct ggtggagact ctatcattga gaactacca                            39


<210>  33
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5B

<400>  33
tggtagttct caatgataga gtctccacca gggtggaag                            39


<210>  34
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 6A

<400>  34
agccttcgac aagctcctgc gaatgcccaa gatc                                 34


<210>  35
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 6B

<400>  35
gatcttgggc attcgcagga gcttgtcgaa ggct                                 34


<210>  36
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 7A

<400>  36
tcgacaagct caagcgagcc gtcaagatca acccctcct                            39


<210>  37
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 7B

<400>  37
aggaggggtt gatcttgacg gctcgcttga gcttgtcga                            39


<210>  38
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 8A

<400>  38
aatgcccaag atcaaccagg cttccgagct gcctcccca                            39


<210>  39
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 8B

<400>  39
tggggaggca gctcggaagc ctggttgatc ttgggcatt                            39


<210>  40
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 9A

<400>  40
tcctccgagc tgcctcagcc tgctgccgtc aacgaag                              37


<210>  41
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 9B

<400>  41
cttcgttgac ggcagcaggc tgaggcagct cggagga                              37


<210>  42
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 10A

<400>  42
aggctgccgt caacgaacag gctgaggatt tccgaaagct                           40


<210>  43
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 10B

<400>  43
agctttcgga aatcctcagc ctgttcgttg acggcagcct                           40


<210>  44
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 11A

<400>  44
aggatttccg aaagctcgcc atcgagctga tcgccactgg                           40


<210>  45
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 11B

<400>  45
ccagtggcga tcagctcgat ggcgagcttt cggaaatcct                           40


<210>  46
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 12A

<400>  46
tcctgctccg atacctgtcc cagttcgctc gaatggccga                           40


<210>  47
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 12B

<400>  47
tcggccattc gagcgaactg ggacaggtat cggagcagg                            39


<210>  48
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 13A

<400>  48
tggccgtgtt cgctcgactg tccgagaagc agcccgct                             38


<210>  49
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 13B

<400>  49
agcgggctgc ttctcggaca gtcgagcgaa cacggcca                             38


<210>  50
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 14A

<400>  50
tcgaatggcc gagaaggtgt acgctggcaa ggctctc                              37


<210>  51
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 14B

<400>  51
gagagccttg ccagcgtaca ccttctcggc cattcga                              37


<210>  52
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 15A

<400>  52
agcccgctgg caaggctcag taagcggccg ccaccgc                              37


<210>  53
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 15B

<400>  53
gcggtggcgg ccgcttactg agccttgcca gcgggct                              37


<210>  54
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 16A

<400>  54
gtactcgtac aagatcgtca ccaccctggg tct                                  33


<210>  55
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 16B

<400>  55
agacccaggg tggtgacgat cttgtacgag tac                                  33


<210>  56
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 17A

<400>  56
actcgtacaa gatctccttc gctctgggtc ttggcgtgct                           40


<210>  57
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 17B

<400>  57
agcacgccaa gacccagagc gaaggagatc ttgtacgagt                           40


<210>  58
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 18A

<400>  58
gcgtgcttgg atacttcatg ctggtccagt accagatg                             38


<210>  59
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 18B

<400>  59
catctggtac tggaccagca tgaagtatcc aagcacgc                             38


<210>  60
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 19A

<400>  60
tggatacttc ctgatgtccc agtaccagat gtac                                 34


<210>  61
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 19B

<400>  61
gtacatctgg tactgggaca tcaggaagta tcca                                 34


<210>  62
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 20A

<400>  62
gatacttcct gatggtctac cagcagatgt acttcattgg                           40


<210>  63
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 20B

<400>  63
ccaatgaagt acatctgctg gtagaccatc aggaagtatc                           40


<210>  64
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 21A

<400>  64
tgatggtcca gtaccacctg tacttcattg gtgc                                 34


<210>  65
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 21B

<400>  65
gcaccaatga agtacaggtg gtactggacc atca                                 34


<210>  66
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 22A

<400>  66
tggtccagta ccagatgcag ttcattggtg ctgtgct                              37


<210>  67
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 22B

<400>  67
agcacagcac caatgaactg catctggtac tggacca                              37


<210>  68
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 23A

<400>  68
cctcttcttt atgcccaaca tcctgacctc gctc                                 34


<210>  69
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 23B

<400>  69
gagcgaggtc aggatgttgg gcataaagaa gagg                                 34


<210>  70
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 24A

<400>  70
tcttctttat gccctcccct atgacctcgc tcctggtg                             38


<210>  71
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 24B

<400>  71
caccaggagc gaggtcatag gggagggcat aaagaaga                             38


<210>  72
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 25A

<400>  72
ttatgccctc catcctggtc gtgctcctgg tgttctttg                            39


<210>  73
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 25B

<400>  73
caaagaacac caggagcacg accaggatgg agggcataa                            39


<210>  74
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 26A

<400>  74
cctccatcct gacctcgatg ctggtgttct ttgtttc                              37


<210>  75
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 26B

<400>  75
gaaacaaaga acaccagcat cgaggtcagg atggagg                              37


<210>  76
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 27A

<400>  76
gacctcgctc ctggtgacct ttgtttccga gctc                                 34


<210>  77
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 27B

<400>  77
gagctcggaa acaaaggtca ccaggagcga ggtc                                 34


<210>  78
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 28A

<400>  78
gctcctggtg ttcttttctt ccgagctcgt cggt                                 34


<210>  79
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 28B

<400>  79
accgacgagc tcggaagaaa agaacaccag gagc                                 34


<210>  80
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 29A

<400>  80
acaacctccc ctaccgaacc actctgcccc atgaaggt                             38


<210>  81
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 29B

<400>  81
accttcatgg ggcagagtgg ttcggtaggg gaggttgt                             38


<210>  82
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 30A

<400>  82
tcccctaccg aaaccctggc atgcatgaag gtctcgtcat                           40


<210>  83
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 30B

<400>  83
atgacgagac cttcatgcat gccagggttt cggtagggga                           40


<210>  84
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 31A

<400>  84
gtgaagtcca agcgactgtc tctgcccctc accatc                               36


<210>  85
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 31B

<400>  85
gatggtgagg ggcagagaca gtcgcttgga cttcac                               36


<210>  86
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 32A

<400>  86
ccaagcgaca ggctctgtgg cagaccatcg acggaactac                           40


<210>  87
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 32B

<400>  87
gtagttccgt cgatggtctg ccacagagcc tgtcgcttgg                           40


<210>  88
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 33A

<400>  88
aactacctac gacgtcttct cttgggtgaa cttccac                              37


<210>  89
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 33B

<400>  89
gtggaagttc acccaagaga agacgtcgta ggtagtt                              37


<210>  90
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 34A

<400>  90
agatgctact gacgcctccc tggttatgca ctctcag                              37


<210>  91
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 34B

<400>  91
ctgagagtgc ataaccaggg aggcgtcagt agcatct                              37


<210>  92
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 35A

<400>  92
ctactgacgc cttcatgttc ctgcactctc aggaagcc                             38


<210>  93
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 35B

<400>  93
ggcttcctga gagtgcagga acatgaaggc gtcagtag                             38


<210>  94
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 36A

<400>  94
tgaaatcatt gagaacttca acggacgaga tgctactga                            39


<210>  95
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 36B

<400>  95
tcagtagcat ctcgtccgtt gaagttctca atgatttca                            39


<210>  96
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 37A

<400>  96
ccttcatggt tatgcacacc aacgaagcct tcgacaagct                           40


<210>  97
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 37B

<400>  97
agcttgtcga aggcttcgtt ggtgtgcata accatgaagg                           40


<210>  98
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 38A

<400>  98
atgcactctc aggaaggcta cgacaagctc aagcga                               36


<210>  99
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 38B

<400>  99
tcgcttgagc ttgtcgtagc cttcctgaga gtgcat                               36


<210>  100
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 39A

<400>  100
ctcaagcgaa tgcccaagct tgatccctcc tttgagct                             38


<210>  101
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 39B

<400>  101
agctcaaagg agggatcaag cttgggcatt cgcttgag                             38


<210>  102
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 40A

<400>  102
tcaacccctc ctttgatgtg cctccccagg ctg                                  33


<210>  103
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 40B

<400>  103
cagcctgggg aggcacatca aaggaggggt tga                                  33


<210>  104
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 41A

<400>  104
tgcctcccca ggctggactc aacgaagctc agg                                  33


<210>  105
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 41B

<400>  105
cctgagcttc gttgagtcca gcctggggag gca                                  33


<210>  106
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 42A

<400>  106
gaaagctccg agaagacatc atcgccactg gcatgt                               36


<210>  107
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 42B

<400>  107
acatgccagt ggcgatgatg tcttctcgga gctttc                               36


<210>  108
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 43A

<400>  108
ccactggcat gtttgaggtc tctcccctct ggtac                                35


<210>  109
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 43B

<400>  109
gtaccagagg ggagagacct caaacatgcc agtgg                                35


<210>  110
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 44A

<400>  110
gtacaagatc tccacctcgg tgggtcttgg cgtgct                               36


<210>  111
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 44B

<400>  111
agcacgccaa gacccaccga ggtggagatc ttgtac                               36


<210>  112
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 45A

<400>  112
ggtcttggcg tgcttgcctt cttcctgatg gtccag                               36


<210>  113
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 45B

<400>  113
ctggaccatc aggaagaagg caagcacgcc aagacc                               36


<210>  114
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 46A

<400>  114
gtacttcatt ggtgctctcg tgctcggtat gcacta                               36


<210>  115
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 46B

<400>  115
tagtgcatac cgagcacgag agcaccaatg aagtac                               36


<210>  116
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 47A

<400>  116
agggtcacga tcccgagctt gataacctgc ctct                                 34


<210>  117
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 47B

<400>  117
agaggcaggt tatcaagctc gggatcgtga ccct                                 34


<210>  118
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 48A

<400>  118
ccaacagtac tatttcgtcc tcatctgtat tctcct                               36


<210>  119
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 48B

<400>  119
aggagaatac agatgaggac gaaatagtac tgttgg                               36


<210>  120
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 49A

<400>  120
ctcgctcctg gtgttcgttc tttccgagct cgtcggt                              37


<210>  121
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 49B

<400>  121
accgacgagc tcggaaagaa cgaacaccag gagcgag                              37


<210>  122
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 50A

<400>  122
gtggcttcgg aattgccgtg atcgtcttca tgaaccacta                           40


<210>  123
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 50B

<400>  123
tagtggttca tgaagacgat cacggcaatt ccgaagccac                           40


<210>  124
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 51A (for 347I to L and 348T to S mutations)

<400>  124
cattcgacga ggcatcctct ctgactggtt ctttgg                               36


<210>  125
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 51B

<400>  125
ccaaagaacc agtcagagag gatgcctcgt cgaatg                               36


<210>  126
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 51A (for 346I to V, 347I to L and 348T to S mutations)

<400>  126
gaacattcga cgaggcgtcc tctctgactg gttctttgg                            39


<210>  127
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 52A

<400>  127
tgccccatga aggtctcatc gtcctgctcc gatacct                              37


<210>  128
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 52B

<400>  128
aggtatcgga gcaggacgat gagaccttca tggggca                              37


<210>  129
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 53A

<400>  129
gtccaagcga caggctgttc ccctcaccat cgacg                                35


<210>  130
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 53B

<400>  130
cgtcgatggt gaggggaaca gcctgtcgct tggac                                35


<210>  131
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 54A

<400>  131
gacggaacta cctacgagat ctccgcttgg gtgaac                               36


<210>  132
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 54B

<400>  132
gttcacccaa gcggagatct cgtaggtagt tccgtc                               36


<210>  133
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 55A

<400>  133
accctggtgg agctgaactg attgagaact accagg                               36


<210>  134
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 55B

<400>  134
cctggtagtt ctcaatcagt tcagctccac cagggt                               36


<210>  135
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 56A

<400>  135
acgagatgct actgacggct acatggttat gcactctc                             38


<210>  136
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 56B

<400>  136
gagagtgcat aaccatgtag ccgtcagtag catctcgt                             38


<210>  137
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 57A

<400>  137
ctcaggaagc cttcgaccga atcaagcgaa tgcccaag                             38


<210>  138
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 57B

<400>  138
cttgggcatt cgcttgattc ggtcgaaggc ttcctgag                             38


<210>  139
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 58A

<400>  139
gcgaatgccc aagatccaac cctcctccga gctgc                                35


<210>  140
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 58B

<400>  140
gcagctcgga ggagggttgg atcttgggca ttcgc                                35


<210>  141
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 59A

<400>  141
cctccgagct gcctcccaac ggtgccgtca acgaagctc                            39


<210>  142
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 59B

<400>  142
gagcttcgtt gacggcaccg ttgggaggca gctcggagg                            39


<210>  143
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 60A

<400>  143
gatcgccact ggcatgtacg acgcctctcc cctctg                               36


<210>  144
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 60B

<400>  144
cagaggggag aggcgtcgta catgccagtg gcgatc                               36


<210>  145
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 61A

<400>  145
ctgtattctc ctgcgaatct tctggtgttt ccagtc                               36


<210>  146
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 61B

<400>  146
gactggaaac accagaagat tcgcaggaga atacag                               36


<210>  147
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 62A

<400>  147
gaaagaggcc attggaatgt ctctgcactg gactct                               36


<210>  148
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 62B

<400>  148
agagtccagt gcagagacat tccaatggcc tctttc                               36


<210>  149
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 63A

<400>  149
tgcactggac tctcaagctg accttccacc tcttctttat                           40


<210>  150
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 63B

<400>  150
ataaagaaga ggtggaaggt cagcttgaga gtccagtgca                           40


<210>  151
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 64A

<400>  151
cacctgtact tcattgctgg tctcgtgctc ggtatg                               36


<210>  152
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 64B

<400>  152
cataccgagc acgagaccag caatgaagta caggtg                               36


<210>  153
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 65A

<400>  153
ttccgagctc gtcggtttcg gcggaattgc catcgtg                              37


<210>  154
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 65B

<400>  154
cacgatggca attccgccga aaccgacgag ctcggaa                              37


<210>  155
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 66A

<400>  155
agttccaaca gtacttctat ctggtcatct gtattc                               36


<210>  156
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 66B

<400>  156
gaatacagat gaccagatag aagtactgtt ggaact                               36


<210>  157
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 67A

<400>  157
atgccctcca tcctgagcct gatgttcgtg ttcgtt                               36


<210>  158
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 67B

<400>  158
aacgaacacg aacatcaggc tcaggatgga gggcat                               36


<210>  159
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 68A

<400>  159
cgaaactgga ataacgtcct gggtctggtc tttg                                 34


<210>  160
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 68B

<400>  160
caaagaccag acccaggacg ttattccagt ttcg                                 34


<210>  161
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 69A

<400>  161
tggtctttgg caacgctgtc cagggcttct ccg                                  33


<210>  162
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 69B

<400>  162
cggagaagcc ctggacagcg ttgccaaaga cca                                  33


<210>  163
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 70A

<400>  163
gccgagaagg tgtacggtgc caaggctcag taagcg                               36


<210>  164
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 70B

<400>  164
cgcttactga gccttggcac cgtacacctt ctcggc                               36


<210>  165
<211>  8560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKO2UFkF2


<220>
<221>  misc_feature
<222>  (692)..(695)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (700)..(700)
<223>  n is a, c, g, or t

<400>  165
catggcgtcc acttcggctc tgcccaagca gaaccctgcg cttagacgca ccgtcacctc     60

aactactgtg acggattctg agtctgccgc cgtctctcct tcagactctc cccgccactc    120

ggcctcttcc acatcgctct cgtccatgtc cgaggttgat atcgccaagc ccaagtccga    180

gtatggtgtc atgctcgaca cctacggcaa ccagttcgag gttcccgact ttaccatcaa    240

ggacatctac aatgccatcc ctaagcactg cttcaagcgc tccgctctca agggatacgg    300

ttatatcctc cgcgacattg tcctcctgac taccactttc agcatctggt acaactttgt    360

gacccccgaa tatatcccct ccacccccgc ccgcgctggt ctgtgggccg tgtacaccgt    420

tcttcagggt cttttcggta ctggtctctg ggttattgcc catgagtgcg gtcacggtgc    480

tttctccgat tctcgcatca tcaacgacat tactggctgg gttcttcact cttccctcct    540

tgtcccctac ttcagctggc aaatctccca ccgaaagcac cacaaggcca ctggcaacat    600

ggagcgtgac atggtcttcg ttccccgaac ccgcgagcag caggctactc gtctcggaaa    660

gatgacccac gagctcgctc atcttactga gnnnntcgtn ggctggccca actacctcat    720

caccaatgtt accggccaca actaccacga gcgccagcgt gagggtcgcg gcaagggcaa    780

gcataacggc ctcggcggtg gtgttaacca cttcgatccc cgcagccctc tgtacgagaa    840

cagtgacgct aagctcatcg tcctcagcga tattggtatc ggtctgatgg ccactgctct    900

gtacttcctc gttcagaagt tcggtttcta caacatggcc atctggtact ttgttcccta    960

cctctgggtt aaccactggc tcgttgccat caccttcctc cagcacaccg accctaccct   1020

tccccactac accaacgacg agtggaactt cgtccgtggt gccgctgcta ccattgaccg   1080

tgagatgggc ttcatcggcc gccaccttct ccacggcatc atcgagactc atgtcctcca   1140

ccactacgtc agcagcatcc ccttctacaa cgcggacgag gccaccgagg ccattaagcc   1200

catcatgggc aagcactacc gggctgatgt ccaggatggt cctcgtggct tcatccgcgc   1260

catgtaccgc agtgcgcgta tgtgccagtg ggttgagccc agcgctggtg ccgagggtgc   1320

tggtaagggt gttctgttct tccgcaaccg caacaacgtg ggcacccccc ccgctgttat   1380

caagcccgtt gcttaagtag gcgcggccgc aagtgtggat ggggaagtga gtgcccggtt   1440

ctgtgtgcac aattggcaat ccaagatgga tggattcaac acagggatat agcgagctac   1500

gtggtggtgc gaggatatag caacggatat ttatgtttga cacttgagaa tgtacgatac   1560

aagcactgtc caagtacaat actaaacata ctgtacatac tcatactcgt acccgggcaa   1620

cggtttcact tgagtgcagt ggctagtgct cttactcgta cagtgtgcaa tactgcgtat   1680

catagtcttt gatgtatatc gtattcattc atgttagttg cgtacgggtg aagcttccac   1740

tggtcggcgt ggtagtgggg cagagtgggg tcggtgtgct gcaggtaggt gatggccacg   1800

agccagtggt tgacccacag gtaggggatc aggtagtaga gggtgacgga agccaggccc   1860

catcggttga tggagtatgc gatgacggac atggtgatac caataccgac gttagagatc   1920

cagatgttga accagtcctt cttctcaaac agcggggcgt tggggttgaa gtggttgaca   1980

gcccatttgt tgagcttggg gtacttctgt ccggtaacgt aagacagcag atacagaggc   2040

catccaaaca cctgctgggt gatgaggccg tagagggtca tgaggggagc gtcctcagca   2100

agctcagacc agtcatgggc gcctcggttc tccataaact cctttcggtc cttgggcaca   2160

aacaccatat cacgggtgag gtgaccagtg gacttgtggt gcatggagtg ggtcagcttc   2220

caggcgtagt aagggaccag catggaggag tgcagaaccc atccggtgac gttgttgacg   2280

gtgttagagt cggagaaagc agagtggcca cactcgtggg caagaaccca cagaccggtg   2340

ccaaacagac cctggacaat ggagtacatg gcccaggcca cagctcggcc ggaagccgag   2400

ggaataagag gcaggtacgc gtaggccatg taggcaaaaa cggcgataaa gaagcaggcg   2460

cgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc   2520

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta   2580

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag   2640

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg   2700

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg   2760

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg   2820

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga   2880

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc   2940

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt   3000

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact   3060

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg   3120

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt   3180

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt   3240

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct   3300

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg   3360

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt   3420

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt   3480

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc   3540

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg   3600

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc   3660

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg   3720

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca   3780

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga   3840

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct   3900

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg   3960

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca   4020

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata   4080

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct   4140

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact   4200

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa   4260

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc   4320

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga   4380

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga   4440

aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat   4500

caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc   4560

tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc   4620

gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac   4680

tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca   4740

ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg   4800

agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag   4860

aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc   4920

accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc   4980

gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag   5040

ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt   5100

gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt   5160

cgcatgcttg aatctacaag taggagggtt ggagtgatta agtgaaactt ctttaacggc   5220

tctatgccag ttctattgat atccgaaaca tcagtatgaa ggtctgataa gggtgacttc   5280

ttcccacaga ttcgtatcag tacgagtacg agaccggtac ttgtaacagt attgatacta   5340

aagggaaact acaacggttg tcagcgtaat gtgacttcgc ccatgaacgc agacacgcag   5400

tgccgagtgc ggtgatatcg cctactcgtt acgtccatgg actacacaac ccctcggctt   5460

cgcttggctt agcctcgggc tcggtgctgt tcagttaaaa cacaatcaaa taacatttct   5520

actttttaga aggcaggccg tcaggagcaa ctccgactcc attgacgttt ctaaacatct   5580

gaatgccttc cttaccttca acaaactggc aggttcgggc gacagtgtaa agagacttga   5640

tgaagttggt gtcgtcgtgt cggtagtgct tgcccatgac cttcttgatc ttctcagtgg   5700

cgattcgggc gttgtagaag ggaattccgt cgtcgcctga gtcgacgagt atctgtctga   5760

ctcgtcattg ccgcctttgg agtacgactc caactatgag tgtgcttgga tcactttgac   5820

gatacattct tcgttggagg ctgtgggtct gacagctgcg ttttcggcgc ggttggccga   5880

caacaatatc agctgcaacg tcattgctgg ctttcatcat gatcacattt ttgtcggcaa   5940

aggcgacgcc cagagagcca ttgacgttct ttctaatttg gaccgatagc cgtatagtcc   6000

agtctatcta taagttcaac taactcgtaa ctattaccat aacatatact tcactgcccc   6060

agataaggtt ccgataaaaa gttctgcaga ctaaatttat ttcagtctcc tcttcaccac   6120

caaaatgccc tcctacgaag ctcgagctaa cgtccacaag tccgcctttg ccgctcgagt   6180

gctcaagctc gtggcagcca agaaaaccaa cctgtgtgct tctctggatg ttaccaccac   6240

caaggagctc attgagcttg ccgataaggt cggaccttat gtgtgcatga tcaaaaccca   6300

tatcgacatc attgacgact tcacctacgc cggcactgtg ctccccctca aggaacttgc   6360

tcttaagcac ggtttcttcc tgttcgagga cagaaagttc gcagatattg gcaacactgt   6420

caagcaccag taccggtgtc accgaatcgc cgagtggtcc gatatcacca acgcccacgg   6480

tgtacccgga accggaatca ttgctggcct gcgagctggt gccgaggaaa ctgtctctga   6540

acagaagaag gaggacgtct ctgactacga gaactcccag tacaaggagt tcctagtccc   6600

ctctcccaac gagaagctgg ccagaggtct gctcatgctg gccgagctgt cttgcaaggg   6660

ctctctggcc actggcgagt actccaagca gaccattgag cttgcccgat ccgaccccga   6720

gtttgtggtt ggcttcattg cccagaaccg acctaagggc gactctgagg actggcttat   6780

tctgaccccc ggggtgggtc ttgacgacaa gggagacgct ctcggacagc agtaccgaac   6840

tgttgaggat gtcatgtcta ccggaacgga tatcataatt gtcggccgag gtctgtacgg   6900

ccagaaccga gatcctattg aggaggccaa gcgataccag aaggctggct gggaggctta   6960

ccagaagatt aactgttaga ggttagacta tggatatgta atttaactgt gtatatagag   7020

agcgtgcaag tatggagcgc ttgttcagct tgtatgatgg tcagacgacc tgtctgatcg   7080

agtatgtatg atactgcaca acctgtgtat ccgcatgatc tgtccaatgg ggcatgttgt   7140

tgtgtttctc gatacggaga tgctgggtac agtgctaata cgttgaacta cttatactta   7200

tatgaggctc gaagaaagct gacttgtgta tgacttaatt aatttgaatc gaatcgatga   7260

gcctaaaatg aacccgagta tatctcataa aattctcggt gagaggtctg tgactgtcag   7320

tacaaggtgc cttcattatg ccctcaacct taccatacct cactgaatgt agtgtacctc   7380

taaaaatgaa atacagtgcc aaaagccaag gcactgagct cgtctaacgg acttgatata   7440

caaccaatta aaacaaatga aaagaaatac agttctttgt atcatttgta acaattaccc   7500

tgtacaaact aaggtattga aatcccacaa tattcccaaa gtccacccct ttccaaattg   7560

tcatgcctac aactcatata ccaagcacta acctaccgtt taaacagtgt acgcagatct   7620

actatagagg aacatttaaa ttgccccgga gaagacggcc aggccgccta gatgacaaat   7680

tcaacaactc acagctgact ttctgccatt gccactaggg gggggccttt ttatatggcc   7740

aagccaagct ctccacgtcg gttgggctgc acccaacaat aaatgggtag ggttgcacca   7800

acaaagggat gggatggggg gtagaagata cgaggataac ggggctcaat ggcacaaata   7860

agaacgaata ctgccattaa gactcgtgat ccagcgactg acaccattgc atcatctaag   7920

ggcctcaaaa ctacctcgga actgctgcgc tgatctggac accacagagg ttccgagcac   7980

tttaggttgc accaaatgtc ccaccaggtg caggcagaaa acgctggaac agcgtgtaca   8040

gtttgtctta acaaaaagtg agggcgctga ggtcgagcag ggtggtgtga cttgttatag   8100

cctttagagc tgcgaaagcg cgtatggatt tggctcatca ggccagattg agggtctgtg   8160

gacacatgtc atgttagtgt acttcaatcg ccccctggat atagccccga caataggccg   8220

tggcctcatt tttttgcctt ccgcacattt ccattgctcg atacccacac cttgcttctc   8280

ctgcacttgc caaccttaat actggtttac attgaccaac atcttacaag cggggggctt   8340

gtctagggta tatataaaca gtggctctcc caatcggttg ccagtctctt ttttcctttc   8400

tttccccaca gattcgaaat ctaaactaca catcacagaa ttccgagccg tgagtatcca   8460

cgacaagatc agtgtcgaga cgacgcgttt tgtgtaatga cacaatccga aagtcgctag   8520

caacacacac tctctacaca aactaaccca gctctggtac                         8560


<210>  166
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  166

His Xaa Xaa Xaa His 
1               5   


<210>  167
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  167

His Xaa Xaa Xaa Xaa His 
1               5       


<210>  168
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  168

His Xaa Xaa His His 
1               5   


<210>  169
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  169

His Xaa Xaa Xaa His His 
1               5       


<210>  170
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  X = His (H) or Gln (Q)

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  170

Xaa Xaa Xaa His His 
1               5   


<210>  171
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  X = His (H) or Gln (Q)

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  171

Xaa Xaa Xaa Xaa His His 
1               5       


<210>  172
<211>  1064
<212>  DNA
<213>  Isochrysis galbana (GenBank Accession No. AF390174)


<220>
<221>  CDS
<222>  (2)..(793)
<223>  delta-9 elongase

<400>  172
g atg gcc ctc gca aac gac gcg gga gag cgc atc tgg gcg gct gtg acc     49
  Met Ala Leu Ala Asn Asp Ala Gly Glu Arg Ile Trp Ala Ala Val Thr       
  1               5                   10                  15            

gac ccg gaa atc ctc att ggc acc ttc tcg tac ttg cta ctc aaa ccg       97
Asp Pro Glu Ile Leu Ile Gly Thr Phe Ser Tyr Leu Leu Leu Lys Pro         
            20                  25                  30                  

ctg ctc cgc aat tcc ggg ctg gtg gat gag aag aag ggc gca tac agg      145
Leu Leu Arg Asn Ser Gly Leu Val Asp Glu Lys Lys Gly Ala Tyr Arg         
        35                  40                  45                      

acg tcc atg atc tgg tac aac gtt ctg ctg gcg ctc ttc tct gcg ctg      193
Thr Ser Met Ile Trp Tyr Asn Val Leu Leu Ala Leu Phe Ser Ala Leu         
    50                  55                  60                          

agc ttc tac gtg acg gcg acc gcc ctc ggc tgg gac tat ggt acg ggc      241
Ser Phe Tyr Val Thr Ala Thr Ala Leu Gly Trp Asp Tyr Gly Thr Gly         
65                  70                  75                  80          

gcg tgg ctg cgc agg caa acc ggc gac aca ccg cag ccg ctc ttc cag      289
Ala Trp Leu Arg Arg Gln Thr Gly Asp Thr Pro Gln Pro Leu Phe Gln         
                85                  90                  95              

tgc ccg tcc ccg gtt tgg gac tcg aag ctc ttc aca tgg acc gcc aag      337
Cys Pro Ser Pro Val Trp Asp Ser Lys Leu Phe Thr Trp Thr Ala Lys         
            100                 105                 110                 

gca ttc tat tac tcc aag tac gtg gag tac ctc gac acg gcc tgg ctg      385
Ala Phe Tyr Tyr Ser Lys Tyr Val Glu Tyr Leu Asp Thr Ala Trp Leu         
        115                 120                 125                     

gtg ctc aag ggc aag agg gtc tcc ttt ctc cag gcc ttc cac cac ttt      433
Val Leu Lys Gly Lys Arg Val Ser Phe Leu Gln Ala Phe His His Phe         
    130                 135                 140                         

ggc gcg ccg tgg gat gtg tac ctc ggc att cgg ctg cac aac gag ggc      481
Gly Ala Pro Trp Asp Val Tyr Leu Gly Ile Arg Leu His Asn Glu Gly         
145                 150                 155                 160         

gta tgg atc ttc atg ttt ttc aac tcg ttc att cac acc atc atg tac      529
Val Trp Ile Phe Met Phe Phe Asn Ser Phe Ile His Thr Ile Met Tyr         
                165                 170                 175             

acc tac tac ggc ctc acc gcc gcc ggg tat aag ttc aag gcc aag ccg      577
Thr Tyr Tyr Gly Leu Thr Ala Ala Gly Tyr Lys Phe Lys Ala Lys Pro         
            180                 185                 190                 

ctc atc acc gcg atg cag atc tgc cag ttc gtg ggc ggc ttc ctg ttg      625
Leu Ile Thr Ala Met Gln Ile Cys Gln Phe Val Gly Gly Phe Leu Leu         
        195                 200                 205                     

gtc tgg gac tac atc aac gtc ccc tgc ttc aac tcg gac aaa ggg aag      673
Val Trp Asp Tyr Ile Asn Val Pro Cys Phe Asn Ser Asp Lys Gly Lys         
    210                 215                 220                         

ttg ttc agc tgg gct ttc aac tat gca tac gtc ggc tcg gtc ttc ttg      721
Leu Phe Ser Trp Ala Phe Asn Tyr Ala Tyr Val Gly Ser Val Phe Leu         
225                 230                 235                 240         

ctc ttc tgc cac ttt ttc tac cag gac aac ttg gca acg aag aaa tcg      769
Leu Phe Cys His Phe Phe Tyr Gln Asp Asn Leu Ala Thr Lys Lys Ser         
                245                 250                 255             

gcc aag gcg ggc aag cag ctc tag gcctcgagcc ggctcgcggg ttcaaggagg     823
Ala Lys Ala Gly Lys Gln Leu                                             
            260                                                         

gcgacacggg ggtgggacgt ttgcatggag atggattgtg gatgtcctta cgccttactc    883

atcaatgtcc tcccatctct cccctctaga ccttctacta gccatctaga agggcagctc    943

agagacggat accgttcccc ctccccttcc ttttcgtctt tgctttgcca ttgtttgttt   1003

gtctctattt tttaaactat tgacgctaac gcgttacgct cgcaaaaaaa aaaaaaaaaa   1063

a                                                                   1064


<210>  173
<211>  263
<212>  PRT
<213>  Isochrysis galbana (GenBank Accession No. AF390174)

<400>  173

Met Ala Leu Ala Asn Asp Ala Gly Glu Arg Ile Trp Ala Ala Val Thr 
1               5                   10                  15      


Asp Pro Glu Ile Leu Ile Gly Thr Phe Ser Tyr Leu Leu Leu Lys Pro 
            20                  25                  30          


Leu Leu Arg Asn Ser Gly Leu Val Asp Glu Lys Lys Gly Ala Tyr Arg 
        35                  40                  45              


Thr Ser Met Ile Trp Tyr Asn Val Leu Leu Ala Leu Phe Ser Ala Leu 
    50                  55                  60                  


Ser Phe Tyr Val Thr Ala Thr Ala Leu Gly Trp Asp Tyr Gly Thr Gly 
65                  70                  75                  80  


Ala Trp Leu Arg Arg Gln Thr Gly Asp Thr Pro Gln Pro Leu Phe Gln 
                85                  90                  95      


Cys Pro Ser Pro Val Trp Asp Ser Lys Leu Phe Thr Trp Thr Ala Lys 
            100                 105                 110         


Ala Phe Tyr Tyr Ser Lys Tyr Val Glu Tyr Leu Asp Thr Ala Trp Leu 
        115                 120                 125             


Val Leu Lys Gly Lys Arg Val Ser Phe Leu Gln Ala Phe His His Phe 
    130                 135                 140                 


Gly Ala Pro Trp Asp Val Tyr Leu Gly Ile Arg Leu His Asn Glu Gly 
145                 150                 155                 160 


Val Trp Ile Phe Met Phe Phe Asn Ser Phe Ile His Thr Ile Met Tyr 
                165                 170                 175     


Thr Tyr Tyr Gly Leu Thr Ala Ala Gly Tyr Lys Phe Lys Ala Lys Pro 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Cys Gln Phe Val Gly Gly Phe Leu Leu 
        195                 200                 205             


Val Trp Asp Tyr Ile Asn Val Pro Cys Phe Asn Ser Asp Lys Gly Lys 
    210                 215                 220                 


Leu Phe Ser Trp Ala Phe Asn Tyr Ala Tyr Val Gly Ser Val Phe Leu 
225                 230                 235                 240 


Leu Phe Cys His Phe Phe Tyr Gln Asp Asn Leu Ala Thr Lys Lys Ser 
                245                 250                 255     


Ala Lys Ala Gly Lys Gln Leu 
            260             


<210>  174
<211>  792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgD9e: synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US-2005-0287652-A1
<311>  2005-06-24
<312>  2005-12-29
<313>  (1)..(792)

<400>  174
atggctctgg ccaacgacgc tggcgagcga atctgggctg ccgtcaccga tcccgaaatc     60

ctcattggca ccttctccta cctgctcctg aagcctctcc tgcgaaactc tggtctcgtg    120

gacgagaaga aaggagccta ccgaacctcc atgatctggt acaacgtcct cctggctctc    180

ttctctgccc tgtccttcta cgtgactgcc accgctctcg gctgggacta cggtactgga    240

gcctggctgc gaagacagac cggtgatact ccccagcctc tctttcagtg tccctctcct    300

gtctgggact ccaagctgtt cacctggact gccaaggcct tctactattc taagtacgtg    360

gagtacctcg acaccgcttg gctggtcctc aagggcaagc gagtgtcctt tctgcaggcc    420

ttccatcact ttggagctcc ctgggacgtc tacctcggca ttcgactgca caacgagggt    480

gtgtggatct tcatgttctt taactcgttc attcacacca tcatgtacac ctactatgga    540

ctgactgccg ctggctacaa gttcaaggcc aagcctctga tcactgccat gcagatttgc    600

cagttcgtcg gtggctttct cctggtctgg gactacatca acgttccctg cttcaactct    660

gacaagggca agctgttctc ctgggctttc aactacgcct acgtcggatc tgtctttctc    720

ctgttctgtc acttctttta ccaggacaac ctggccacca agaaatccgc taaggctggt    780

aagcagcttt ag                                                        792


<210>  175
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(777)
<223>  delta-9 elongase; U.S. Patent Application No. 60/739989, filed 
       11/23/2005

<400>  175
atggaggtgg tgaatgaaat agtctcaatt gggcaggaag ttttacccaa agttgattat     60

gcccaactct ggagtgatgc cagtcactgt gaggtgcttt acttgtccat cgcatttgtc    120

atcttgaagt tcactcttgg cccccttggt ccaaaaggtc agtctcgtat gaagtttgtt    180

ttcaccaatt acaaccttct catgtccatt tattcgttgg gatcattcct ctcaatggca    240

tatgccatgt acaccatcgg tgttatgtct gacaactgcg agaaggcttt tgacaacaac    300

gtcttcagga tcaccacgca gttgttctat ttgagcaagt tcctggagta tattgactcc    360

ttctatttgc cactgatggg caagcctctg acctggttgc aattcttcca tcatttgggg    420

gcaccgatgg atatgtggct gttctataat taccgaaatg aagctgtttg gatttttgtg    480

ctgttgaatg gtttcatcca ctggatcatg tacggttatt attggaccag attgatcaag    540

ctgaagttcc ccatgccaaa atccctgatt acatcaatgc agatcattca attcaatgtt    600

ggtttctaca ttgtctggaa gtacaggaac attccctgtt atcgccaaga tgggatgagg    660

atgtttggct ggttcttcaa ttacttttat gttggcacag tcttgtgttt gttcttgaat    720

ttctatgtgc aaacgtatat cgtcaggaag cacaagggag ccaaaaagat tcagtga       777


<210>  176
<211>  258
<212>  PRT
<213>  Euglena gracilis


<220>
<221>  MISC_FEATURE
<222>  (1)..(258)
<223>  delta-9 elongase; U.S. Patent Application No. 60/739989, filed 
       11/23/2005

<400>  176

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  177
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(777)
<223>  synthetic delta-9 elongase codon-optimized for Yarrowia 
       lipolytica; U.S. Patent Application No. 60/739989, filed 
       11/23/2005

<400>  177
atggaggtcg tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat     60

gctcagctct ggtctgatgc ctcgcactgc gaggtgctgt acctctccat cgccttcgtc    120

atcctgaagt tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg    180

ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc    240

tacgccatgt acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat    300

gtcttccgaa tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc    360

ttctatctgc ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga    420

gctcctatgg acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg    480

ctgctcaacg gcttcattca ctggatcatg tacggctact attggacccg actgatcaag    540

ctcaagttcc ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt    600

ggcttctaca tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga    660

atgtttggct ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac    720

ttctacgtgc agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtga       777


<210>  178
<211>  120
<212>  PRT
<213>  Saccharomyces cerevisiae (GenBank Accession No. P40312)

<400>  178

Met Pro Lys Val Tyr Ser Tyr Gln Glu Val Ala Glu His Asn Gly Pro 
1               5                   10                  15      


Glu Asn Phe Trp Ile Ile Ile Asp Asp Lys Val Tyr Asp Val Ser Gln 
            20                  25                  30          


Phe Lys Asp Glu His Pro Gly Gly Asp Glu Ile Ile Met Asp Leu Gly 
        35                  40                  45              


Gly Gln Asp Ala Thr Glu Ser Phe Val Asp Ile Gly His Ser Asp Glu 
    50                  55                  60                  


Ala Leu Arg Leu Leu Lys Gly Leu Tyr Ile Gly Asp Val Asp Lys Thr 
65                  70                  75                  80  


Ser Glu Arg Val Ser Val Glu Lys Val Ser Thr Ser Glu Asn Gln Ser 
                85                  90                  95      


Lys Gly Ser Gly Thr Leu Val Val Ile Leu Ala Ile Leu Met Leu Gly 
            100                 105                 110         


Val Ala Tyr Tyr Leu Leu Asn Glu 
        115                 120 


<210>  179
<211>  124
<212>  PRT
<213>  Schizosaccharomyces pombe (GenBank Accession No. O94391)

<400>  179

Met Ser Val Lys Tyr Phe Glu Pro Glu Glu Ile Val Glu His Asn Asn 
1               5                   10                  15      


Ser Lys Asp Met Tyr Met Val Ile Asn Gly Lys Val Tyr Asp Val Ser 
            20                  25                  30          


Asn Phe Ala Asp Asp His Pro Gly Gly Leu Asp Ile Met Leu Asp Tyr 
        35                  40                  45              


Ala Gly Gln Asp Ala Thr Lys Ala Tyr Gln Asp Ile Gly His Ser Ile 
    50                  55                  60                  


Ala Ala Asp Glu Leu Leu Glu Glu Met Tyr Ile Gly Asp Leu Lys Pro 
65                  70                  75                  80  


Gly Thr Glu Glu Arg Leu Lys Glu Leu Lys Lys Pro Arg Ser Phe Asp 
                85                  90                  95      


Asn Asp Thr Pro Pro Leu Pro Leu Leu Ile Ala Leu Ile Val Leu Pro 
            100                 105                 110         


Ala Ile Ala Val Ile Val Phe Val Lys Leu Asn Lys 
        115                 120                 


<210>  180
<211>  8739
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY116

<400>  180
ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc gggtggacgt     60

ctagaggtac ctagcaatta acagatagtt tgccggtgat aattctctta acctcccaca    120

ctcctttgac ataacgattt atgtaacgaa actgaaattt gaccagatat tgtgtccgcg    180

gtggagctcc agcttttgtt ccctttagtg agggtttaaa cgagcttggc gtaatcatgg    240

tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cgtacgagcc    300

ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg    360

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc    420

ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact    480

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta    540

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag    600

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc    660

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta    720

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg    780

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc    840

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac    900

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac    960

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg   1020

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga   1080

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt   1140

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag   1200

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct   1260

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg   1320

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat   1380

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc   1440

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg   1500

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct   1560

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca   1620

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg   1680

ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg   1740

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc   1800

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag   1860

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg   1920

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag   1980

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat   2040

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg   2100

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca   2160

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca   2220

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat   2280

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag   2340

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc   2400

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt   2460

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc   2520

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta   2580

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc   2640

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg   2700

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt   2760

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat   2820

tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg   2880

aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg   2940

caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg   3000

ccagtgaatt gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg   3060

tcgatggtgt cgataagctt gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa   3120

ggaaacctaa ttctacatcc gagagactgc cgagatccag tctacactga ttaattttcg   3180

ggccaataat ttaaaaaaat cgtgttatat aatattatat gtattatata tatacatcat   3240

gatgatactg acagtcatgt cccattgcta aatagacaga ctccatctgc cgcctccaac   3300

tgatgttctc aatatttaag gggtcatctc gcattgttta ataataaaca gactccatct   3360

accgcctcca aatgatgttc tcaaaatata ttgtatgaac ttatttttat tacttagtat   3420

tattagacaa cttacttgct ttatgaaaaa cacttcctat ttaggaaaca atttataatg   3480

gcagttcgtt catttaacaa tttatgtaga ataaatgtta taaatgcgta tgggaaatct   3540

taaatatgga tagcataaat gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa   3600

aaaatccctt gtacaacata aatagtcatc gagaaatatc aactatcaaa gaacagctat   3660

tcacacgtta ctattgagat tattattgga cgagaatcac acactcaact gtctttctct   3720

cttctagaaa tacaggtaca agtatgtact attctcattg ttcatacttc tagtcatttc   3780

atcccacata ttccttggat ttctctccaa tgaatgacat tctatcttgc aaattcaaca   3840

attataataa gatataccaa agtagcggta tagtggcaat caaaaagctt ctctggtgtg   3900

cttctcgtat ttatttttat tctaatgatc cattaaaggt atatatttat ttcttgttat   3960

ataatccttt tgtttattac atgggctgga tacataaagg tattttgatt taattttttg   4020

cttaaattca atcccccctc gttcagtgtc aactgtaatg gtaggaaatt accatacttt   4080

tgaagaagca aaaaaaatga aagaaaaaaa aaatcgtatt tccaggttag acgttccgca   4140

gaatctagaa tgcggtatgc ggtacattgt tcttcgaacg taaaagttgc gctccctgag   4200

atattgtaca tttttgcttt tacaagtaca agtacatcgt acaactatgt actactgttg   4260

atgcatccac aacagtttgt tttgtttttt tttgtttttt ttttttctaa tgattcatta   4320

ccgctatgta tacctacttg tacttgtagt aagccgggtt attggcgttc aattaatcat   4380

agacttatga atctgcacgg tgtgcgctgc gagttacttt tagcttatgc atgctacttg   4440

ggtgtaatat tgggatctgt tcggaaatca acggatgctc aaccgatttc gacagtaatt   4500

aattaatttg aatcgaatcg gagcctaaaa tgaacccgag tatatctcat aaaattctcg   4560

gtgagaggtc tgtgactgtc agtacaaggt gccttcatta tgccctcaac cttaccatac   4620

ctcactgaat gtagtgtacc tctaaaaatg aaatacagtg ccaaaagcca aggcactgag   4680

ctcgtctaac ggacttgata tacaaccaat taaaacaaat gaaaagaaat acagttcttt   4740

gtatcatttg taacaattac cctgtacaaa ctaaggtatt gaaatcccac aatattccca   4800

aagtccaccc ctttccaaat tgtcatgcct acaactcata taccaagcac taacctacca   4860

aacaccacta aaaccccaca aaatatatct taccgaatat acagtaacaa gctaccacca   4920

cactcgttgg gtgcagtcgc cagcttaaag atatctatcc acatcagcca caactccctt   4980

cctttaataa accgactaca cccttggcta ttgaggttat gagtgaatat actgtagaca   5040

agacactttc aagaagactg tttccaaaac gtaccactgt cctccactac aaacacaccc   5100

aatctgcttc ttctagtcaa ggttgctaca ccggtaaatt ataaatcatc atttcattag   5160

cagggcaggg ccctttttat agagtcttat acactagcgg accctgccgg tagaccaacc   5220

cgcaggcgcg tcagtttgct ccttccatca atgcgtcgta gaaacgactt actccttctt   5280

gagcagctcc ttgaccttgt tggcaacaag tctccgacct cggaggtgga ggaagagcct   5340

ccgatatcgg cggtagtgat accagcctcg acggactcct tgacggcagc ctcaacagcg   5400

tcaccggcgg gcttcatgtt aagagagaac ttgagcatca tggcggcaga cagaatggtg   5460

gcaatggggt tgaccttctg cttgccgaga tcgggggcag atccgtgaca gggctcgtac   5520

agaccgaacg cctcgttggt gtcgggcaga gaagccagag aggcggaggg cagcagaccc   5580

agagaaccgg ggatgacgga ggcctcgtcg gagatgatat cgccaaacat gttggtggtg   5640

atgatgatac cattcatctt ggagggctgc ttgatgagga tcatggcggc cgagtcgatc   5700

agctggtggt tgagctcgag ctgggggaat tcgtccttga ggactcgagt gacagtcttt   5760

cgccaaagtc gagaggaggc cagcacgttg gccttgtcaa gagaccacac gggaagaggg   5820

gggttgtgct gaagggccag gaaggcggcc attcgggcaa ttcgctcaac ctcaggaacg   5880

gagtaggtct cggtgtcgga agcgacgcca gatccgtcat cctcctttcg ctctccaaag   5940

tagatacctc cgacgagctc tcggacaatg atgaagtcgg tgccctcaac gtttcggatg   6000

ggggagagat cggcgagctt gggcgacagc agctggcagg gtcgcaggtt ggcgtacagg   6060

ttcaggtcct ttcgcagctt gaggagaccc tgctcgggtc gcacgtcggt tcgtccgtcg   6120

ggagtggtcc atacggtgtt ggcagcgcct ccgacagcac cgagcataat agagtcagcc   6180

tttcggcaga tgtcgagagt agcgtcggtg atgggctcgc cctccttctc aatggcagct   6240

cctccaatga gtcggtcctc aaacacaaac tcggtgccgg aggcctcagc aacagacttg   6300

agcaccttga cggcctcggc aatcacctcg gggccacaga agtcgccgcc gagaagaaca   6360

atcttcttgg agtcagtctt ggtcttctta gtttcgggtt ccattgtgga tgtgtgtggt   6420

tgtatgtgtg atgtggtgtg tggagtgaaa atctgtggct ggcaaacgct cttgtatata   6480

tacgcacttt tgcccgtgct atgtggaaga ctaaacctcc gaagattgtg actcaggtag   6540

tgcggtatcg gctagggacc caaaccttgt cgatgccgat agcgctatcg aacgtacccc   6600

agccggccgg gagtatgtcg gaggggacat acgagatcgt caagggtttg tggccaactg   6660

gtatttaaat gtagctaacg gtagcaggcg aactactggt acatacctcc cccggaatat   6720

gtacaggcat aatgcgtatc tgtgggacat gtggtcgttg cgccattatg taagcagcgt   6780

gtactcctct gactgtccat atggtttgct ccatctcacc ctcatcgttt tcattgttca   6840

caggcggcca caaaaaaact gtcttctctc cttctctctt cgccttagtc tactcggacc   6900

agttttagtt tagcttggcg ccactggata aatgagacct caggccttgt gatgaggagg   6960

tcacttatga agcatgttag gaggtgcttg tatggataga gaagcaccca aaataataag   7020

aataataata aaacaggggg cgttgtcatt tcatatcgtg ttttcaccat caatacacct   7080

ccaaacaatg cccttcatgt ggccagcccc aatattgtcc tgtagttcaa ctctatgcag   7140

ctcgtatctt attgagcaag taaaactctg tcagccgata ttgcccgacc cgcgacaagg   7200

gtcaacaagg tggtgtaagg ccttcgcaga agtcaaaact gtgccaaaca aacatctaga   7260

gtctctttgg tgtttctcgc atatatttwa tcggctgtct tacgtatttg cgcctcggta   7320

ccggactaat ttcggatcat ccccaatacg ctttttcttc gcagctgtca acagtgtcca   7380

tgatctatcc acctaaatgg gtcatatgag gcgtataatt tcgtggtgct gataataatt   7440

cccatatatt tgacacaaaa cttccccccc tagacataca tctcacaatc tcacttcttg   7500

tgcttctgtc acacatctcc tccagctgac ttcaactcac acctctgccc cagttggtct   7560

acagcggtat aaggtttctc cgcatagagg tgcaccactc ctcccgatac ttgtttgtgt   7620

gacttgtggg tcacgacata tatatctaca cacattgcgc caccctttgg ttcttccagc   7680

acaacaaaaa cacgacacgc taaccatggc caatttactg accgtacacc aaaatttgcc   7740

tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag   7800

ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg   7860

ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg   7920

cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt   7980

gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc   8040

tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa   8100

acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag   8160

cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt   8220

acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag   8280

aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc   8340

acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga   8400

tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc   8460

caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat   8520

ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg   8580

tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc   8640

tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac   8700

aggggcaatg gtgcgcctgc tggaagatgg cgattaagc                          8739


<210>  181
<211>  15304
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKO2UF8289


<220>
<221>  misc_feature
<222>  (5601)..(5601)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (5606)..(5609)
<223>  n is a, c, g, or t

<400>  181
cgatcgagga agaggacaag cggctgcttc ttaagtttgt gacatcagta tccaaggcac     60

cattgcaagg attcaaggct ttgaacccgt catttgccat tcgtaacgct ggtagacagg    120

ttgatcggtt ccctacggcc tccacctgtg tcaatcttct caagctgcct gactatcagg    180

acattgatca acttcggaag aaacttttgt atgccattcg atcacatgct ggtttcgatt    240

tgtcttagag gaacgcatat acagtaatca tagagaataa acgatattca tttattaaag    300

tagatagttg aggtagaagt tgtaaagagt gataaatagc ggccgctcac tgaatctttt    360

tggctccctt gtgcttcctg acgatatacg tttgcacata gaaattcaag aacaaacaca    420

agactgtgcc aacataaaag taattgaaga accagccaaa catcctcatc ccatcttggc    480

gataacaggg aatgttcctg tacttccaga caatgtagaa accaacattg aattgaatga    540

tctgcattga tgtaatcagg gattttggca tggggaactt cagcttgatc aatctggtcc    600

aataataacc gtacatgatc cagtggatga aaccattcaa cagcacaaaa atccaaacag    660

cttcatttcg gtaattatag aacagccaca tatccatcgg tgcccccaaa tgatggaaga    720

attgcaacca ggtcagaggc ttgcccatca gtggcaaata gaaggagtca atatactcca    780

ggaacttgct caaatagaac aactgcgtgg tgatcctgaa gacgttgttg tcaaaagcct    840

tctcgcagtt gtcagacata acaccgatgg tgtacatggc atatgccatt gagaggaatg    900

atcccaacga ataaatggac atgagaaggt tgtaattggt gaaaacaaac ttcatacgag    960

actgaccttt tggaccaagg gggccaagag tgaacttcaa gatgacaaat gcgatggaca   1020

agtaaagcac ctcacagtga ctggcatcac tccagagttg ggcataatca actggttggg   1080

taaaacttcc tgcccaattg agactatttc attcaccacc tccatggtta gcgtgtcgtg   1140

tttttgttgt gctggaagaa ccaaagggtg gcgcaatgtg tgtagatata tatgtcgtga   1200

cccacaagtc acacaaacaa gtatcgggag gagtggtgca cctctatgcg gagaaacctt   1260

ataccgctgt agaccaactg gggcagaggt gtgagttgaa gtcagctgga ggagatgtgt   1320

gacagaagca caagaagtga gattgtgaga tgtatgtcta gggggggaag ttttgtgtca   1380

aatatatggg aattattatc agcaccacga aattatacgc ctcatatgac ccatttaggt   1440

ggatagatca tggacactgt tgacagctgc gaagaaaaag cgtattgggg atgatccgaa   1500

attagtccgg taccgaggcg caaatacgta agacagccga twaaatatat gcgagaaaca   1560

ccaaagagac tctagatgtt tgtttggcac agttttgact tctgcgaagg ccttacacca   1620

ccttgttgac ccttgtcgcg ggtcgggcaa tatcggctga cagagtttta cttgctcaat   1680

aagatacgag ctgcatagag ttgaactaca ggacaatatt ggggctggcc acatgaaggg   1740

cattgtttgg aggtgtattg atggtgaaaa cacgatatga aatgacaacg ccccctgttt   1800

tattattatt cttattattt tgggtgcttc tctatccata caagcacctc ctaacatgct   1860

tcataagtga cctcctcatc acaaggcctg aggtctcatt tatccagtgg cgccaagcta   1920

aactaaaact ggtccgagta gactaaggcg aagagagaag gagagaagac agtttttttg   1980

tggccgcctg tgaacaatga aaacgatgag ggtgagatgg agcaaaccat atggtttaaa   2040

cagtcagagg agtacacgct gcttacataa tggcgcaacg accacatgtc ccacagatac   2100

gcatcgattc gattcaaatt aattaaaagg cgttgaaaca gaatgagcca gacagcaagg   2160

acaaggtggc caacagcaag gagtccaaaa agccctctat tgacgagatc cacgatgtta   2220

ttgctcatga ggtttccgag ctcgatgctg ggaagaagaa gtgatttgta tataagaaat   2280

aaatgagata tagtaaagga gtgcaagaga atggcaaggt ggtcaaattc tatattactt   2340

gcagtcactg gttcctcgtt gacatgaatg aagttaccgt tggcatagct gatttaatat   2400

ataactgtcc aactaactct cacctagata taacccatgt gtgtgtttcc aatcatcaat   2460

gcggccgctt actgagcctt ggcaccgggc tgcttctcgg ccattcgagc gaactgggac   2520

aggtatcgga gcaggatgac gagaccttca tggggcagag ggtttcggta ggggaggttg   2580

tgcttctggc acagctgttc cacctggtag gaaacggcag tgaggttgtg tcgaggcagg   2640

gtgggccaga gatggtgctc gatctggtag ttcaggcctc caaagaacca gtcagtaatg   2700

atgcctcgtc gaatgttcat ggtctcatgg atctgaccca cagagaagcc atgtccgtcc   2760

cagacggaat caccgatctt ctccagaggg tagtggttca tgaagaccac gatggcaatt   2820

ccgaagccac cgacgagctc ggaaacaaag aacaccagca tcgaggtcag gatggagggc   2880

ataaagaaga ggtggaacag ggtcttgaga gtccagtgca gagcgagtcc aatggcctct   2940

ttcttgtact gagatcggta gaactggttg tctcggtcct tgagggatcg aacggtcagc   3000

acagactgga aacaccagat gaatcgcagg agaatacaga tgaccaggaa atagtactgt   3060

tggaactgaa tgagctttcg ggagatggga gaagctcgag tgacatcgtc ctcggaccag   3120

gcgagcagag gcaggttatc aatgtcggga tcgtgaccct gaacgttggt agcagaatga   3180

tgggcgttgt gtctgtcctt ccaccaggtc acggagaagc cctggagtcc gttgccaaag   3240

accagaccca ggacgttatt ccagtttcgg ttcttgaagg tctggtggtg gcagatgtca   3300

tgagacagcc atcccatttg ctggtagtgc ataccgagca cgagagcacc aatgaagtac   3360

aggtggtact ggaccagcat gaagaaggca agcacgccaa gacccagggt ggtcaagatc   3420

ttgtacgagt accagagggg agaggcgtca aacatgccag tggcgatcag ctcttctcgg   3480

agctttcgga aatcctcctg agcttcgttg acggcagcct ggggaggcag ctcggaagcc   3540

tggttgatct tgggcattcg cttgagcttg tcgaaggctt cctgagagtg cataaccatg   3600

aaggcgtcag tagcatctcg tccctggtag ttctcaatga tttcagctcc accagggtgg   3660

aagttcaccc aagcggagac gtcgtacacc tttccgtcga tgacgagggg cagagcctgt   3720

cgagaagcct tcaccatggc cattgctgta gatatgtctt gtgtgtaagg gggttggggt   3780

ggttgtttgt gttcttgact tttgtgttag caagggaaga cgggcaaaaa agtgagtgtg   3840

gttgggaggg agagacgagc cttatatata atgcttgttt gtgtttgtgc aagtggacgc   3900

cgaaacgggc aggagccaaa ctaaacaagg cagacaatgc gagcttaatt ggattgcctg   3960

atgggcaggg gttagggctc gatcaatggg ggtgcgaagt gacaaaattg ggaattaggt   4020

tcgcaagcaa ggctgacaag actttggccc aaacatttgt acgcggtgga caacaggagc   4080

cacccatcgt ctgtcacggg ctagccggtc gtgcgtcctg tcaggctcca cctaggctcc   4140

atgccactcc atacaatccc actagtgtac cgctaggccg cttttagctc ccatctaaga   4200

cccccccaaa acctccactg tacagtgcac tgtactgtgt ggcgatcaag ggcaagggaa   4260

aaaaggcgca aacatgcacg catggaatga cgtaggtaag gcgttactag actgaaaagt   4320

ggcacatttc ggcgtgccaa agggtcctag gtgcgtttcg cgagctgggc gccaggccaa   4380

gccgctccaa aacgcctctc cgactccctc cagcggcctc catatcccca tccctctcca   4440

cagcaatgtt gttaagcctt gcaaacgaaa aaatagaaag gctaataagc ttccaatatt   4500

gtggtgtacg ctgcataacg caacaatgag cgccaaacaa cacacacaca cagcacacag   4560

cagcattaac cacgatgttt aaacagtgta cgcagatccc gtcaacagtt ttatatatcg   4620

tagttacaac catcaacact ttttggtaag tgtaccattc tatactccaa ctggtctgca   4680

actgtacaag tagacatgtt aatggtagtt aataacatct acagcagaac ctatggtaaa   4740

gacattgcat ttttacagga agtatcgtcc tacacgttga taaatccaaa gatgcggaac   4800

ttcttccact tttatcatca tcccctactc gtacactcgt actctttgtt cgatcgcgat   4860

tcatttctat aaataatctt gtatgtacat gcggccgcgc ctacttaagc aacgggcttg   4920

ataacagcgg ggggggtgcc cacgttgttg cggttgcgga agaacagaac acccttacca   4980

gcaccctcgg caccagcgct gggctcaacc cactggcaca tacgcgcact gcggtacatg   5040

gcgcggatga agccacgagg accatcctgg acatcagccc ggtagtgctt gcccatgatg   5100

ggcttaatgg cctcggtggc ctcgtccgcg ttgtagaagg ggatgctgct gacgtagtgg   5160

tggaggacat gagtctcgat gatgccgtgg agaaggtggc ggccgatgaa gcccatctca   5220

cggtcaatgg tagcagcggc accacggacg aagttccact cgtcgttggt gtagtgggga   5280

agggtagggt cggtgtgctg gaggaaggtg atggcaacga gccagtggtt aacccagagg   5340

tagggaacaa agtaccagat ggccatgttg tagaaaccga acttctgaac gaggaagtac   5400

agagcagtgg ccatcagacc gataccaata tcgctgagga cgatgagctt agcgtcactg   5460

ttctcgtaca gagggctgcg gggatcgaag tggttaacac caccgccgag gccgttatgc   5520

ttgcccttgc cgcgaccctc acgctggcgc tcgtggtagt tgtggccggt aacattggtg   5580

atgaggtagt tgggccagcc nacgannnnc tcagtaagat gagcgagctc gtgggtcatc   5640

tttccgagac gagtagcctg ctgctcgcgg gttcggggaa cgaagaccat gtcacgctcc   5700

atgttgccag tggccttgtg gtgctttcgg tgggagattt gccagctgaa gtaggggaca   5760

aggagggaag agtgaagaac ccagccagta atgtcgttga tgatgcgaga atcggagaaa   5820

gcaccgtgac cgcactcatg ggcaataacc cagagaccag taccgaaaag accctgaaga   5880

acggtgtaca cggcccacag accagcgcgg gcgggggtgg aggggatata ttcgggggtc   5940

acaaagttgt accagatgct gaaagtggta gtcaggagga caatgtcgcg gaggatataa   6000

ccgtatccct tgagagcgga gcgcttgaag cagtgcttag ggatggcatt gtagatgtcc   6060

ttgatggtaa agtcgggaac ctcgaactgg ttgccgtagg tgtcgagcat gacaccatac   6120

tcggacttgg gcttggcgat atcaacctcg gacatggacg agagcgatgt ggaagaggcc   6180

gagtggcggg gagagtctga aggagagacg gcggcagact cagaatccgt cacagtagtt   6240

gaggtgacgg tgcgtctaag cgcagggttc tgcttgggca gagccgaagt ggacgccatg   6300

gttgtgaatt agggtggtga gaatggttgg ttgtagggaa gaatcaaagg ccggtctcgg   6360

gatccgtggg tatatatata tatatatata tatacgatcc ttcgttacct ccctgttctc   6420

aaaactgtgg tttttcgttt ttcgtttttt gctttttttg atttttttag ggccaactaa   6480

gcttccagat ttcgctaatc acctttgtac taattacaag aaaggaagaa gctgattaga   6540

gttgggcttt ttatgcaact gtgctactcc ttatctctga tatgaaagtg tagacccaat   6600

cacatcatgt catttagagt tggtaatact gggaggatag ataaggcacg aaaacgagcc   6660

atagcagaca tgctgggtgt agccaagcag aagaaagtag atgggagcca attgacgagc   6720

gagggagcta cgccaatccg acatacgaca cgctgagatc gtcttggccg gggggtacct   6780

acagatgtcc aagggtaagt gcttgactgt aattgtatgt ctgaggacaa atatgtagtc   6840

agccgtataa agtcatacca ggcaccagtg ccatcatcga accactaact ctctatgata   6900

catgcctccg gtattattgt accatgcgtc gctttgttac atacgtatct tgcctttttc   6960

tctcagaaac tccagacttt ggctattggt cgagataagc ccggaccata gtgagtcttt   7020

cacactctac atttctccct tgctccaact atttaaattg ccccggagaa gacggccagg   7080

ccgcctagat gacaaattca acaactcaca gctgactttc tgccattgcc actagggggg   7140

ggccttttta tatggccaag ccaagctctc cacgtcggtt gggctgcacc caacaataaa   7200

tgggtagggt tgcaccaaca aagggatggg atggggggta gaagatacga ggataacggg   7260

gctcaatggc acaaataaga acgaatactg ccattaagac tcgtgatcca gcgactgaca   7320

ccattgcatc atctaagggc ctcaaaacta cctcggaact gctgcgctga tctggacacc   7380

acagaggttc cgagcacttt aggttgcacc aaatgtccca ccaggtgcag gcagaaaacg   7440

ctggaacagc gtgtacagtt tgtcttaaca aaaagtgagg gcgctgaggt cgagcagggt   7500

ggtgtgactt gttatagcct ttagagctgc gaaagcgcgt atggatttgg ctcatcaggc   7560

cagattgagg gtctgtggac acatgtcatg ttagtgtact tcaatcgccc cctggatata   7620

gccccgacaa taggccgtgg cctcattttt ttgccttccg cacatttcca ttgctcggta   7680

cccacacctt gcttctcctg cacttgccaa ccttaatact ggtttacatt gaccaacatc   7740

ttacaagcgg ggggcttgtc tagggtatat ataaacagtg gctctcccaa tcggttgcca   7800

gtctcttttt tcctttcttt ccccacagat tcgaaatcta aactacacat cacagaattc   7860

cgagccgtga gtatccacga caagatcagt gtcgagacga cgcgttttgt gtaatgacac   7920

aatccgaaag tcgctagcaa cacacactct ctacacaaac taacccagct ctggtaccat   7980

ggtgaaggct tctcgacagg ctctgcccct cgtcatcgac ggaaaggtgt acgacgtctc   8040

cgcttgggtg aacttccacc ctggtggagc tgaaatcatt gagaactacc agggacgaga   8100

tgctactgac gccttcatgg ttatgcactc tcaggaagcc ttcgacaagc tcaagcgaat   8160

gcccaagatc aaccaggctt ccgagctgcc tccccaggct gccgtcaacg aagctcagga   8220

ggatttccga aagctccgag aagagctgat cgccactggc atgtttgacg cctctcccct   8280

ctggtactcg tacaagatct tgaccaccct gggtcttggc gtgcttgcct tcttcatgct   8340

ggtccagtac cacctgtact tcattggtgc tctcgtgctc ggtatgcact accagcaaat   8400

gggatggctg tctcatgaca tctgccacca ccagaccttc aagaaccgaa actggaataa   8460

cgtcctgggt ctggtctttg gcaacggact ccagggcttc tccgtgacct ggtggaagga   8520

cagacacaac gcccatcatt ctgctaccaa cgttcagggt cacgatcccg acattgataa   8580

cctgcctctg ctcgcctggt ccgaggacga tgtcactcga gcttctccca tctcccgaaa   8640

gctcattcag ttccaacagt actatttcct ggtcatctgt attctcctgc gattcatctg   8700

gtgtttccag tctgtgctga ccgttcgatc cctcaaggac cgagacaacc agttctaccg   8760

atctcagtac aagaaagagg ccattggact cgctctgcac tggactctca agaccctgtt   8820

ccacctcttc tttatgccct ccatcctgac ctcgatgctg gtgttctttg tttccgagct   8880

cgtcggtggc ttcggaattg ccatcgtggt cttcatgaac cactaccctc tggagaagat   8940

cggtgattcc gtctgggacg gacatggctt ctctgtgggt cagatccatg agaccatgaa   9000

cattcgacga ggcatcatta ctgactggtt ctttggaggc ctgaactacc agatcgagca   9060

ccatctctgg cccaccctgc ctcgacacaa cctcactgcc gtttcctacc aggtggaaca   9120

gctgtgccag aagcacaacc tcccctaccg aaaccctctg ccccatgaag gtctcgtcat   9180

cctgctccga tacctgtccc agttcgctcg aatggccgag aagcagcccg gtgccaaggc   9240

tcagtaagcg gccgcaagtg tggatgggga agtgagtgcc cggttctgtg tgcacaattg   9300

gcaatccaag atggatggat tcaacacagg gatatagcga gctacgtggt ggtgcgagga   9360

tatagcaacg gatatttatg tttgacactt gagaatgtac gatacaagca ctgtccaagt   9420

acaatactaa acatactgta catactcata ctcgtacccg ggcaacggtt tcacttgagt   9480

gcagtggcta gtgctcttac tcgtacagtg tgcaatactg cgtatcatag tctttgatgt   9540

atatcgtatt cattcatgtt agttgcgtac gggtgaagct tccactggtc ggcgtggtag   9600

tggggcagag tggggtcggt gtgctgcagg taggtgatgg ccacgagcca gtggttgacc   9660

cacaggtagg ggatcaggta gtagagggtg acggaagcca ggccccatcg gttgatggag   9720

tatgcgatga cggacatggt gataccaata ccgacgttag agatccagat gttgaaccag   9780

tccttcttct caaacagcgg ggcgttgggg ttgaagtggt tgacagccca tttgttgagc   9840

ttggggtact tctgtccggt aacgtaagac agcagataca gaggccatcc aaacacctgc   9900

tgggtgatga ggccgtagag ggtcatgagg ggagcgtcct cagcaagctc agaccagtca   9960

tgggcgcctc ggttctccat aaactccttt cggtccttgg gcacaaacac catatcacgg  10020

gtgaggtgac cagtggactt gtggtgcatg gagtgggtca gcttccaggc gtagtaaggg  10080

accagcatgg aggagtgcag aacccatccg gtgacgttgt tgacggtgtt agagtcggag  10140

aaagcagagt ggccacactc gtgggcaaga acccacagac cggtgccaaa cagaccctgg  10200

acaatggagt acatggccca ggccacagct cggccggaag ccgagggaat aagaggcagg  10260

tacgcgtagg ccatgtaggc aaaaacggcg ataaagaagc aggcgcgcca gctgcattaa  10320

tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg  10380

ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag  10440

gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa  10500

ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc  10560

cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca  10620

ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg  10680

accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct  10740

catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt  10800

gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag  10860

tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc  10920

agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac  10980

actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga  11040

gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc  11100

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg  11160

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca  11220

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt  11280

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca  11340

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg  11400

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca  11460

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt  11520

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt  11580

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca  11640

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca  11700

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga  11760

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact  11820

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga  11880

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg  11940

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc  12000

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga  12060

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat  12120

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt  12180

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt  12240

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgat  12300

gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc  12360

gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa  12420

taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt  12480

gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg  12540

cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt  12600

ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga  12660

gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg  12720

ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg  12780

cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag  12840

ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa  12900

ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca  12960

gtgaattgta atacgactca ctatagggcg aattgggccc gacgtcgcat gcttgaatct  13020

acaagtagga gggttggagt gattaagtga aacttcttta acggctctat gccagttcta  13080

ttgatatccg aaacatcagt atgaaggtct gataagggtg acttcttccc acagattcgt  13140

atcagtacga gtacgagacc ggtacttgta acagtattga tactaaaggg aaactacaac  13200

ggttgtcagc gtaatgtgac ttcgcccatg aacgcagaca cgcagtgccg agtgcggtga  13260

tatcgcctac tcgttacgtc catggactac acaacccctc ggcttcgctt ggcttagcct  13320

cgggctcggt gctgttcagt taaaacacaa tcaaataaca tttctacttt ttagaaggca  13380

ggccgtcagg agcaactccg actccattga cgtttctaaa catctgaatg ccttccttac  13440

cttcaacaaa ctggcaggtt cgggcgacag tgtaaagaga cttgatgaag ttggtgtcgt  13500

cgtgtcggta gtgcttgccc atgaccttct tgatcttctc agtggcgatt cgggcgttgt  13560

agaagggaat tcctttacct gcaggataac ttcgtataat gtatgctata cgaagttatg  13620

atctctctct tgagcttttc cataacaagt tcttctgcct ccaggaagtc catgggtggt  13680

ttgatcatgg ttttggtgta gtggtagtgc agtggtggta ttgtgactgg ggatgtagtt  13740

gagaataagt catacacaag tcagctttct tcgagcctca tataagtata agtagttcaa  13800

cgtattagca ctgtacccag catctccgta tcgagaaaca caacaacatg ccccattgga  13860

cagatcatgc ggatacacag gttgtgcagt atcatacata ctcgatcaga caggtcgtct  13920

gaccatcata caagctgaac aagcgctcca tacttgcacg ctctctatat acacagttaa  13980

attacatatc catagtctaa cctctaacag ttaatcttct ggtaagcctc ccagccagcc  14040

ttctggtatc gcttggcctc ctcaatagga tctcggttct ggccgtacag acctcggccg  14100

acaattatga tatccgttcc ggtagacatg acatcctcaa cagttcggta ctgctgtccg  14160

agagcgtctc ccttgtcgtc aagacccacc ccgggggtca gaataagcca gtcctcagag  14220

tcgcccttag gtcggttctg ggcaatgaag ccaaccacaa actcggggtc ggatcgggca  14280

agctcaatgg tctgcttgga gtactcgcca gtggccagag agcccttgca agacagctcg  14340

gccagcatga gcagacctct ggccagcttc tcgttgggag aggggactag gaactccttg  14400

tactgggagt tctcgtagtc agagacgtcc tccttcttct gttcagagac agtttcctcg  14460

gcaccagctc gcaggccagc aatgattccg gttccgggta caccgtgggc gttggtgata  14520

tcggaccact cggcgattcg gtgacaccgg tactggtgct tgacagtgtt gccaatatct  14580

gcgaactttc tgtcctcgaa caggaagaaa ccgtgcttaa gagcaagttc cttgaggggg  14640

agcacagtgc cggcgtaggt gaagtcgtca atgatgtcga tatgggtttt gatcatgcac  14700

acataaggtc cgaccttatc ggcaagctca atgagctcct tggtggtggt aacatccaga  14760

gaagcacaca ggttggtttt cttggctgcc acgagcttga gcactcgagc ggcaaaggcg  14820

gacttgtgga cgttagctcg agcttcgtag gagggcattt tggtggtgaa gaggagactg  14880

aaataaattt agtctgcaga actttttatc ggaaccttat ctggggcagt gaagtatatg  14940

ttatggtaat agttacgagt tagttgaact tatagataga ctggactata cggctatcgg  15000

tccaaattag aaagaacgtc aatggctctc tgggcgtcgc ctttgccgac aaaaatgtga  15060

tcatgatgaa agccagcaat gacgttgcag ctgatattgt tgtcggccaa ccgcgccgaa  15120

aacgcagctg tcagacccac agcctccaac gaagaatgta tcgtcaaagt gatccaagca  15180

cactcatagt tggagtcgta ctccaaaggc ggcaatgacg agtcagacag atactcgtcg  15240

acgcgataac ttcgtataat gtatgctata cgaagttatc gtacgatagt tagtagacaa  15300

caat                                                               15304


<210>  182
<211>  1288
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified mutant EgD8S-23, comprising a 5' Not1 site

<400>  182
gcggccgcac catggtgaag gcttctcgac aggctctgcc cctcgtcatc gacggaaagg     60

tgtacgacgt ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact    120

accagggacg agatgctact gacgccttca tggttatgca ctctcaggaa gccttcgaca    180

agctcaagcg aatgcccaag atcaaccagg cttccgagct gcctccccag gctgccgtca    240

acgaagctca ggaggatttc cgaaagctcc gagaagagct gatcgccact ggcatgtttg    300

acgcctctcc cctctggtac tcgtacaaga tcttgaccac cctgggtctt ggcgtgcttg    360

ccttcttcat gctggtccag taccacctgt acttcattgg tgctctcgtg ctcggtatgc    420

actaccagca aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc    480

gaaactggaa taacgtcctg ggtctggtct ttggcaacgg actccagggc ttctccgtga    540

cctggtggaa ggacagacac aacgcccatc attctgctac caacgttcag ggtcacgatc    600

ccgacattga taacctgcct ctgctcgcct ggtccgagga cgatgtcact cgagcttctc    660

ccatctcccg aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc    720

tgcgattcat ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca    780

accagttcta ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc    840

tcaagaccct gttccacctc ttctttatgc cctccatcct gacctcgatg ctggtgttct    900

ttgtttccga gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg aaccactacc    960

ctctggagaa gatcggtgat tccgtctggg acggacatgg cttctctgtg ggtcagatcc   1020

atgagaccat gaacattcga cgaggcatca ttactgactg gttctttgga ggcctgaact   1080

accagatcga gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct   1140

accaggtgga acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg   1200

aaggtctcgt catcctgctc cgatacctgt cccagttcgc tcgaatggcc gagaagcagc   1260

ccggtgccaa ggctcagtaa gcggccgc                                      1288


<210>  183
<211>  5252
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKR457


<220>
<221>  misc_feature
<222>  (3872)..(3872)
<223>  n is a, c, g, or t

<400>  183
gtacgtgggc ggatcccccg ggctgcagga attcactggc cgtcgtttta caacgtcgtg     60

actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca    120

gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga    180

atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc    240

gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac    300

acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca    360

gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga    420

aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa    480

taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt    540

gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa    600

tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta    660

ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag    720

taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca    780

gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta    840

aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc    900

gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc    960

ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca   1020

ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc   1080

acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca   1140

taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac   1200

tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg   1260

cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg   1320

ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg   1380

gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac   1440

gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc   1500

aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct   1560

aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc   1620

actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc   1680

gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg   1740

atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa   1800

atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc   1860

ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt   1920

gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa   1980

cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc   2040

tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc   2100

cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct   2160

ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat   2220

gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc   2280

tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg   2340

ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc   2400

gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg   2460

cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca   2520

gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact   2580

ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa   2640

acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactcg acgtacgtcc   2700

tcgaagagaa gggttaataa cacatttttt aacattttta acacaaattt tagttattta   2760

aaaatttatt aaaaaattta aaataagaag aggaactctt taaataaatc taacttacaa   2820

aatttatgat ttttaataag ttttcaccaa taaaaaatgt cataaaaata tgttaaaaag   2880

tatattatca atattctctt tatgataaat aaaaagaaaa aaaaaataaa agttaagtga   2940

aaatgagatt gaagtgactt taggtgtgta taaatatatc aaccccgcca acaatttatt   3000

taatccaaat atattgaagt atattattcc atagccttta tttatttata tatttattat   3060

ataaaagctt tatttgttct aggttgttca tgaaatattt ttttggtttt atctccgttg   3120

taagaaaatc atgtgctttg tgtcgccact cactattgca gctttttcat gcattggtca   3180

gattgacggt tgattgtatt tttgtttttt atggttttgt gttatgactt aagtcttcat   3240

ctctttatct cttcatcagg tttgatggtt acctaatatg gtccatgggt acatgcatgg   3300

ttaaattagg tggccaactt tgttgtgaac gatagaattt tttttatatt aagtaaacta   3360

tttttatatt atgaaataat aataaaaaaa atattttatc attattaaca aaatcatatt   3420

agttaatttg ttaactctat aataaaagaa atactgtaac attcacatta catggtaaca   3480

tctttccacc ctttcatttg ttttttgttt gatgactttt tttcttgttt aaatttattt   3540

cccttctttt aaatttggaa tacattatca tcatatataa actaaaatac taaaaacagg   3600

attacacaaa tgataaataa taacacaaat atttataaat ctagctgcaa tatatttaaa   3660

ctagctatat cgatattgta aaataaaact agctgcattg atactgataa aaaaatatca   3720

tgtgctttct ggactgatga tgcagtatac ttttgacatt gcctttattt tatttttcag   3780

aaaagctttc ttagttctgg gttcttcatt atttgtttcc catctccatt gtgaattgaa   3840

tcatttgctt cgtgtcacaa atacaattta gntaggtaca tgcattggtc agattcacgg   3900

tttattatgt catgacttaa gttcatggta gtacattacc tgccacgcat gcattatatt   3960

ggttagattt gataggcaaa tttggttgtc aacaatataa atataaataa tgtttttata   4020

ttacgaaata acagtgatca aaacaaacag ttttatcttt attaacaaga ttttgttttt   4080

gtttgatgac gttttttaat gtttacgctt tcccccttct tttgaattta gaacacttta   4140

tcatcataaa atcaaatact aaaaaaatta catatttcat aaataataac acaaatattt   4200

ttaaaaaatc tgaaataata atgaacaata ttacatatta tcacgaaaat tcattaataa   4260

aaatattata taaataaaat gtaatagtag ttatatgtag gaaaaaagta ctgcacgcat   4320

aatatataca aaaagattaa aatgaactat tataaataat aacactaaat taatggtgaa   4380

tcatatcaaa ataatgaaaa agtaaataaa atttgtaatt aacttctata tgtattacac   4440

acacaaataa taaataatag taaaaaaaat tatgataaat atttaccatc tcataagata   4500

tttaaaataa tgataaaaat atagattatt ttttatgcaa ctagctagcc aaaaagagaa   4560

cacgggtata tataaaaaga gtacctttaa attctactgt acttccttta ttcctgacgt   4620

ttttatatca agtggacata cgtgaagatt ttaattatca gtctaaatat ttcattagca   4680

cttaatactt ttctgtttta ttcctatcct ataagtagtc ccgattctcc caacattgct   4740

tattcacaca actaactaag aaagtcttcc atagcccccc aagcggccgc gacacaagtg   4800

tgagagtact aaataaatgc tttggttgta cgaaatcatt acactaaata aaataatcaa   4860

agcttatata tgccttccgc taaggccgaa tgcaaagaaa ttggttcttt ctcgttatct   4920

tttgccactt ttactagtac gtattaatta ctacttaatc atctttgttt acggctcatt   4980

atatccggtc tagaggatcc aaggccgcga agttaaaagc aatgttgtca cttgtcgtac   5040

taacacatga tgtgatagtt tatgctagct agctataaca taagctgtct ctgagtgtgt   5100

tgtatattaa taaagatcat cactggtgaa tggtgatcgt gtacgtaccc tacttagtag   5160

gcaatggaag cacttagagt gtgctttgtg catggccttg cctctgtttt gagacttttg   5220

taatgttttc gagtttaaat ctttgccttt gc                                 5252


<210>  184
<211>  6532
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKR1058


<220>
<221>  misc_feature
<222>  (4340)..(4340)
<223>  n is a, c, g, or t

<400>  184
ggccgcgaca caagtgtgag agtactaaat aaatgctttg gttgtacgaa atcattacac     60

taaataaaat aatcaaagct tatatatgcc ttccgctaag gccgaatgca aagaaattgg    120

ttctttctcg ttatcttttg ccacttttac tagtacgtat taattactac ttaatcatct    180

ttgtttacgg ctcattatat ccggtctaga ggatccaagg ccgcgaagtt aaaagcaatg    240

ttgtcacttg tcgtactaac acatgatgtg atagtttatg ctagctagct ataacataag    300

ctgtctctga gtgtgttgta tattaataaa gatcatcact ggtgaatggt gatcgtgtac    360

gtaccctact tagtaggcaa tggaagcact tagagtgtgc tttgtgcatg gccttgcctc    420

tgttttgaga cttttgtaat gttttcgagt ttaaatcttt gcctttgcgt acgtgggcgg    480

atcccccggg ctgcaggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc    540

ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata    600

gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc    660

gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca    720

ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac    780

ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga    840

ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac    900

gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt    960

agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct   1020

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat   1080

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg   1140

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg   1200

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc   1260

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat   1320

gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact   1380

attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca   1440

tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact   1500

tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg   1560

atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg   1620

agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg   1680

aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg   1740

caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag   1800

ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc   1860

gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga   1920

tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat   1980

atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc   2040

tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag   2100

accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct   2160

gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac   2220

caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc   2280

tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg   2340

ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt   2400

tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt   2460

gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc   2520

tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca   2580

gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata   2640

gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg   2700

ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct   2760

ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta   2820

ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag   2880

tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga   2940

ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg   3000

caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg   3060

ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc   3120

atgattacgc caagcttgca tgcctgcagg tcgactcgac gtacgtcctc gaagagaagg   3180

gttaataaca cattttttaa catttttaac acaaatttta gttatttaaa aatttattaa   3240

aaaatttaaa ataagaagag gaactcttta aataaatcta acttacaaaa tttatgattt   3300

ttaataagtt ttcaccaata aaaaatgtca taaaaatatg ttaaaaagta tattatcaat   3360

attctcttta tgataaataa aaagaaaaaa aaaataaaag ttaagtgaaa atgagattga   3420

agtgacttta ggtgtgtata aatatatcaa ccccgccaac aatttattta atccaaatat   3480

attgaagtat attattccat agcctttatt tatttatata tttattatat aaaagcttta   3540

tttgttctag gttgttcatg aaatattttt ttggttttat ctccgttgta agaaaatcat   3600

gtgctttgtg tcgccactca ctattgcagc tttttcatgc attggtcaga ttgacggttg   3660

attgtatttt tgttttttat ggttttgtgt tatgacttaa gtcttcatct ctttatctct   3720

tcatcaggtt tgatggttac ctaatatggt ccatgggtac atgcatggtt aaattaggtg   3780

gccaactttg ttgtgaacga tagaattttt tttatattaa gtaaactatt tttatattat   3840

gaaataataa taaaaaaaat attttatcat tattaacaaa atcatattag ttaatttgtt   3900

aactctataa taaaagaaat actgtaacat tcacattaca tggtaacatc tttccaccct   3960

ttcatttgtt ttttgtttga tgactttttt tcttgtttaa atttatttcc cttcttttaa   4020

atttggaata cattatcatc atatataaac taaaatacta aaaacaggat tacacaaatg   4080

ataaataata acacaaatat ttataaatct agctgcaata tatttaaact agctatatcg   4140

atattgtaaa ataaaactag ctgcattgat actgataaaa aaatatcatg tgctttctgg   4200

actgatgatg cagtatactt ttgacattgc ctttatttta tttttcagaa aagctttctt   4260

agttctgggt tcttcattat ttgtttccca tctccattgt gaattgaatc atttgcttcg   4320

tgtcacaaat acaatttagn taggtacatg cattggtcag attcacggtt tattatgtca   4380

tgacttaagt tcatggtagt acattacctg ccacgcatgc attatattgg ttagatttga   4440

taggcaaatt tggttgtcaa caatataaat ataaataatg tttttatatt acgaaataac   4500

agtgatcaaa acaaacagtt ttatctttat taacaagatt ttgtttttgt ttgatgacgt   4560

tttttaatgt ttacgctttc ccccttcttt tgaatttaga acactttatc atcataaaat   4620

caaatactaa aaaaattaca tatttcataa ataataacac aaatattttt aaaaaatctg   4680

aaataataat gaacaatatt acatattatc acgaaaattc attaataaaa atattatata   4740

aataaaatgt aatagtagtt atatgtagga aaaaagtact gcacgcataa tatatacaaa   4800

aagattaaaa tgaactatta taaataataa cactaaatta atggtgaatc atatcaaaat   4860

aatgaaaaag taaataaaat ttgtaattaa cttctatatg tattacacac acaaataata   4920

aataatagta aaaaaaatta tgataaatat ttaccatctc ataagatatt taaaataatg   4980

ataaaaatat agattatttt ttatgcaact agctagccaa aaagagaaca cgggtatata   5040

taaaaagagt acctttaaat tctactgtac ttcctttatt cctgacgttt ttatatcaag   5100

tggacatacg tgaagatttt aattatcagt ctaaatattt cattagcact taatactttt   5160

ctgttttatt cctatcctat aagtagtccc gattctccca acattgctta ttcacacaac   5220

taactaagaa agtcttccat agccccccaa gcggccgcac catggtgaag gcttctcgac   5280

aggctctgcc cctcgtcatc gacggaaagg tgtacgacgt ctccgcttgg gtgaacttcc   5340

accctggtgg agctgaaatc attgagaact accagggacg agatgctact gacgccttca   5400

tggttatgca ctctcaggaa gccttcgaca agctcaagcg aatgcccaag atcaaccagg   5460

cttccgagct gcctccccag gctgccgtca acgaagctca ggaggatttc cgaaagctcc   5520

gagaagagct gatcgccact ggcatgtttg acgcctctcc cctctggtac tcgtacaaga   5580

tcttgaccac cctgggtctt ggcgtgcttg ccttcttcat gctggtccag taccacctgt   5640

acttcattgg tgctctcgtg ctcggtatgc actaccagca aatgggatgg ctgtctcatg   5700

acatctgcca ccaccagacc ttcaagaacc gaaactggaa taacgtcctg ggtctggtct   5760

ttggcaacgg actccagggc ttctccgtga cctggtggaa ggacagacac aacgcccatc   5820

attctgctac caacgttcag ggtcacgatc ccgacattga taacctgcct ctgctcgcct   5880

ggtccgagga cgatgtcact cgagcttctc ccatctcccg aaagctcatt cagttccaac   5940

agtactattt cctggtcatc tgtattctcc tgcgattcat ctggtgtttc cagtctgtgc   6000

tgaccgttcg atccctcaag gaccgagaca accagttcta ccgatctcag tacaagaaag   6060

aggccattgg actcgctctg cactggactc tcaagaccct gttccacctc ttctttatgc   6120

cctccatcct gacctcgatg ctggtgttct ttgtttccga gctcgtcggt ggcttcggaa   6180

ttgccatcgt ggtcttcatg aaccactacc ctctggagaa gatcggtgat tccgtctggg   6240

acggacatgg cttctctgtg ggtcagatcc atgagaccat gaacattcga cgaggcatca   6300

ttactgactg gttctttgga ggcctgaact accagatcga gcaccatctc tggcccaccc   6360

tgcctcgaca caacctcact gccgtttcct accaggtgga acagctgtgc cagaagcaca   6420

acctccccta ccgaaaccct ctgccccatg aaggtctcgt catcctgctc cgatacctgt   6480

cccagttcgc tcgaatggcc gagaagcagc ccggtgccaa ggctcagtaa gc           6532


<210>  185
<211>  7887
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKR607

<400>  185
ggccgcagta tatcttaaat tctttaatac ggtgtactag gatattgaac tggttcttga     60

tgatgaaaac ctgggccgag attgcagcta tttatagtca taggtcttgt taacatgcat    120

ggacatttgg ccacggggtg gcatgcagtt tgacgggtgt tgaaataaac aaaaatgagg    180

tggcggaaga gaatacgagt ttgaggttgg gttagaaaca acaaatgtga gggctcatga    240

tgggttgagt tggtgaatgt tttgggctgc tcgattgaca cctttgtgag tacgtgttgt    300

tgtgcatggc ttttggggtc cagttttttt ttcttgacgc ggcgatcctg atcagctagt    360

ggataagtga tgtccactgt gtgtgattgc gtttttgttt gaattttatg aacttagaca    420

ttgctatgca aaggatactc tcattgtgtt ttgtcttctt ttgttccttg gctttttctt    480

atgatccaag agactagtca gtgttgtggc attcgagact accaagatta attatgatgg    540

gggaaggata agtaactgat tagtacggac tgttaccaaa ttaattaata agcggcaaat    600

gaagggcatg gatcaaaagc ttggatctcc tgcaggatct ggccggccgg atctcgtacg    660

gatccgtcga cggcgcgccc gatcatccgg atatagttcc tcctttcagc aaaaaacccc    720

tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag    780

ccaactcagc ttcctttcgg gctttgttag cagccggatc gatccaagct gtacctcact    840

attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta    900

cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc    960

cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat   1020

tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga   1080

gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca   1140

tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga   1200

acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt   1260

tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca   1320

tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc   1380

agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac   1440

cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga   1500

tcgcatccat agcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt   1560

cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt   1620

ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat   1680

aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc   1740

ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga   1800

cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt   1860

ccatgggtat atctccttct taaagttaaa caaaattatt tctagaggga aaccgttgtg   1920

gtctccctat agtgagtcgt attaatttcg cgggatcgag atcgatccaa ttccaatccc   1980

acaaaaatct gagcttaaca gcacagttgc tcctctcaga gcagaatcgg gtattcaaca   2040

ccctcatatc aactactacg ttgtgtataa cggtccacat gccggtatat acgatgactg   2100

gggttgtaca aaggcggcaa caaacggcgt tcccggagtt gcacacaaga aatttgccac   2160

tattacagag gcaagagcag cagctgacgc gtacacaaca agtcagcaaa cagacaggtt   2220

gaacttcatc cccaaaggag aagctcaact caagcccaag agctttgcta aggccctaac   2280

aagcccacca aagcaaaaag cccactggct cacgctagga accaaaaggc ccagcagtga   2340

tccagcccca aaagagatct cctttgcccc ggagattaca atggacgatt tcctctatct   2400

ttacgatcta ggaaggaagt tcgaaggtga aggtgacgac actatgttca ccactgataa   2460

tgagaaggtt agcctcttca atttcagaaa gaatgctgac ccacagatgg ttagagaggc   2520

ctacgcagca ggtctcatca agacgatcta cccgagtaac aatctccagg agatcaaata   2580

ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaattgca tcaagaacac   2640

agagaaagac atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt   2700

gcttcataaa ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcctactga   2760

atctaaggcc atgcatggag tctaagattc aaatcgagga tctaacagaa ctcgccgtga   2820

agactggcga acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg   2880

tcaacatggt ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag   2940

aagaccaaag ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat   3000

tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct   3060

acaaatgcca tcattgcgat aaaggaaagg ctatcattca agatgcctct gccgacagtg   3120

gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca   3180

cgtcttcaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat gacgcacaat   3240

cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga   3300

cacgctcgag ctcatttctc tattacttca gccataacaa aagaactctt ttctcttctt   3360

attaaaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa   3420

agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca   3480

gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct   3540

acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc   3600

ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg   3660

tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg   3720

ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac   3780

cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc   3840

atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc   3900

tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg   3960

atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga   4020

gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt   4080

ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag   4140

gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct   4200

tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc   4260

gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga   4320

ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga   4380

gggcaaagga atagtgaggt acctaaagaa ggagtgcgtc gaagcagatc gttcaaacat   4440

ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata   4500

atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat   4560

gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa   4620

aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg   4680

atgtcgaatc gatcaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc   4740

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc   4800

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata   4860

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg   4920

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct   4980

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa   5040

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc   5100

tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt   5160

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg   5220

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg   5280

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct   5340

tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc   5400

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg   5460

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc   5520

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt   5580

aagggatttt ggtcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc   5640

tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca   5700

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg   5760

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc   5820

accatatgga catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca   5880

tacacatacg atttaggtga cactatagaa cggcgcgcca agcttgttga aacatccctg   5940

aagtgtctca ttttatttta tttattcttt gctgataaaa aaataaaata aaagaagcta   6000

agcacacggt caaccattgc tctactgcta aaagggttat gtgtagtgtt ttactgcata   6060

aattatgcag caaacaagac aactcaaatt aaaaaatttc ctttgcttgt ttttttgttg   6120

tctctgactt gactttcttg tggaagttgg ttgtataagg attgggacac cattgtcctt   6180

cttaatttaa ttttattctt tgctgataaa aaaaaaaatt tcatatagtg ttaaataata   6240

atttgttaaa taaccaaaaa gtcaaatatg tttactctcg tttaaataat tgagattcgt   6300

ccagcaaggc taaacgattg tatagattta tgacaatatt tactttttta tagataaatg   6360

ttatattata ataaatttat atacatatat tatatgttat ttattattat tttaaatcct   6420

tcaatatttt atcaaaccaa ctcataattt tttttttatc tgtaagaagc aataaaatta   6480

aatagaccca ctttaaggat gatccaacct ttatacagag taagagagtt caaatagtac   6540

cctttcatat acatatcaac taaaatatta gaaatatcat ggatcaaacc ttataaagac   6600

attaaataag tggataagta taatatataa atgggtagta tataatatat aaatggatac   6660

aaacttctct ctttataatt gttatgtctc cttaacatcc taatataata cataagtggg   6720

taatatataa tatataaatg gagacaaact tcttccatta taattgttat gtcttcttaa   6780

cacttatgtc tcgttcacaa tgctaaggtt agaattgttt agaaagtctt atagtacaca   6840

tttgtttttg tactatttga agcattccat aagccgtcac gattcagatg atttataata   6900

ataagaggaa atttatcata gaacaataag gtgcatagat agagtgttaa tatatcataa   6960

catcctttgt ttattcatag aagaagtgag atggagctca gttattatac tgttacatgg   7020

tcggatacaa tattccatgc tctccatgag ctcttacacc tacatgcatt ttagttcata   7080

cttgcggccg ctaaagctgc ttaccagcct tagcggattt cttggtggcc aggttgtcct   7140

ggtaaaagaa gtgacagaac aggagaaaga cagatccgac gtaggcgtag ttgaaagccc   7200

aggagaacag cttgcccttg tcagagttga agcagggaac gttgatgtag tcccagacca   7260

ggagaaagcc accgacgaac tggcaaatct gcatggcagt gatcagaggc ttggccttga   7320

acttgtagcc agcggcagtc agtccatagt aggtgtacat gatggtgtga atgaacgagt   7380

taaagaacat gaagatccac acaccctcgt tgtgcagtcg aatgccgagg tagacgtccc   7440

agggagctcc aaagtgatgg aaggcctgca gaaaggacac tcgcttgccc ttgaggacca   7500

gccaagcggt gtcgaggtac tccacgtact tagaatagta gaaggccttg gcagtccagg   7560

tgaacagctt ggagtcccag acaggagagg gacactgaaa gagaggctgg ggagtatcac   7620

cggtctgtct tcgcagccag gctccagtac cgtagtccca gccgagagcg gtggcagtca   7680

cgtagaagga cagggcagag aagagagcca ggaggacgtt gtaccagatc atggaggttc   7740

ggtaggctcc tttcttctcg tccacgagac cagagtttcg caggagaggc ttcaggagca   7800

ggtaggagaa ggtgccaatg aggatttcgg gatcggtgac ggcagcccag attcgctcgc   7860

cagcgtcgtt ggccagagcc atggtgc                                       7887


<210>  186
<211>  11766
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKR1060


<220>
<221>  misc_feature
<222>  (10571)..(10571)
<223>  n is a, c, g, or t

<400>  186
ggagatccaa gcttttgatc catgcccttc atttgccgct tattaattaa tttggtaaca     60

gtccgtacta atcagttact tatccttccc ccatcataat taatcttggt agtctcgaat    120

gccacaacac tgactagtct cttggatcat aagaaaaagc caaggaacaa aagaagacaa    180

aacacaatga gagtatcctt tgcatagcaa tgtctaagtt cataaaattc aaacaaaaac    240

gcaatcacac acagtggaca tcacttatcc actagctgat caggatcgcc gcgtcaagaa    300

aaaaaaactg gaccccaaaa gccatgcaca acaacacgta ctcacaaagg tgtcaatcga    360

gcagcccaaa acattcacca actcaaccca tcatgagccc tcacatttgt tgtttctaac    420

ccaacctcaa actcgtattc tcttccgcca cctcattttt gtttatttca acacccgtca    480

aactgcatgc caccccgtgg ccaaatgtcc atgcatgtta acaagaccta tgactataaa    540

tagctgcaat ctcggcccag gttttcatca tcaagaacca gttcaatatc ctagtacacc    600

gtattaaaga atttaagata tactgcggcc gcaccatggc tctggccaac gacgctggcg    660

agcgaatctg ggctgccgtc accgatcccg aaatcctcat tggcaccttc tcctacctgc    720

tcctgaagcc tctcctgcga aactctggtc tcgtggacga gaagaaagga gcctaccgaa    780

cctccatgat ctggtacaac gtcctcctgg ctctcttctc tgccctgtcc ttctacgtga    840

ctgccaccgc tctcggctgg gactacggta ctggagcctg gctgcgaaga cagaccggtg    900

atactcccca gcctctcttt cagtgtccct ctcctgtctg ggactccaag ctgttcacct    960

ggactgccaa ggccttctac tattctaagt acgtggagta cctcgacacc gcttggctgg   1020

tcctcaaggg caagcgagtg tcctttctgc aggccttcca tcactttgga gctccctggg   1080

acgtctacct cggcattcga ctgcacaacg agggtgtgtg gatcttcatg ttctttaact   1140

cgttcattca caccatcatg tacacctact atggactgac tgccgctggc tacaagttca   1200

aggccaagcc tctgatcact gccatgcaga tttgccagtt cgtcggtggc tttctcctgg   1260

tctgggacta catcaacgtt ccctgcttca actctgacaa gggcaagctg ttctcctggg   1320

ctttcaacta cgcctacgtc ggatctgtct ttctcctgtt ctgtcacttc ttttaccagg   1380

acaacctggc caccaagaaa tccgctaagg ctggtaagca gctttagcgg ccgcaagtat   1440

gaactaaaat gcatgtaggt gtaagagctc atggagagca tggaatattg tatccgacca   1500

tgtaacagta taataactga gctccatctc acttcttcta tgaataaaca aaggatgtta   1560

tgatatatta acactctatc tatgcacctt attgttctat gataaatttc ctcttattat   1620

tataaatcat ctgaatcgtg acggcttatg gaatgcttca aatagtacaa aaacaaatgt   1680

gtactataag actttctaaa caattctaac cttagcattg tgaacgagac ataagtgtta   1740

agaagacata acaattataa tggaagaagt ttgtctccat ttatatatta tatattaccc   1800

acttatgtat tatattagga tgttaaggag acataacaat tataaagaga gaagtttgta   1860

tccatttata tattatatac tacccattta tatattatac ttatccactt atttaatgtc   1920

tttataaggt ttgatccatg atatttctaa tattttagtt gatatgtata tgaaagggta   1980

ctatttgaac tctcttactc tgtataaagg ttggatcatc cttaaagtgg gtctatttaa   2040

ttttattgct tcttacagat aaaaaaaaaa ttatgagttg gtttgataaa atattgaagg   2100

atttaaaata ataataaata acatataata tatgtatata aatttattat aatataacat   2160

ttatctataa aaaagtaaat attgtcataa atctatacaa tcgtttagcc ttgctggacg   2220

aatctcaatt atttaaacga gagtaaacat atttgacttt ttggttattt aacaaattat   2280

tatttaacac tatatgaaat tttttttttt atcagcaaag aataaaatta aattaagaag   2340

gacaatggtg tcccaatcct tatacaacca acttccacaa gaaagtcaag tcagagacaa   2400

caaaaaaaca agcaaaggaa attttttaat ttgagttgtc ttgtttgctg cataatttat   2460

gcagtaaaac actacacata acccttttag cagtagagca atggttgacc gtgtgcttag   2520

cttcttttat tttatttttt tatcagcaaa gaataaataa aataaaatga gacacttcag   2580

ggatgtttca acaagcttgg cgcgccgttc tatagtgtca cctaaatcgt atgtgtatga   2640

tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtcc atatggtgca   2700

ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac   2760

ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga   2820

ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac   2880

gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgaccaaa atcccttaac   2940

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag   3000

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg   3060

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca   3120

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga   3180

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca   3240

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc   3300

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca   3360

ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa   3420

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc   3480

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc   3540

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg   3600

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat   3660

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca   3720

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca   3780

aaccgcctct ccccgcgcgt tggccgattc attaatgcag gttgatcgat tcgacatcga   3840

tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg   3900

ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata   3960

aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat   4020

atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg   4080

tttgaacgat ctgcttcgac gcactccttc tttaggtacc tcactattcc tttgccctcg   4140

gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc   4200

agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga   4260

cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc   4320

tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg   4380

caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg   4440

gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc   4500

agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg   4560

cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag   4620

cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg   4680

gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc   4740

cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct   4800

ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac   4860

cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca   4920

cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga   4980

aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga   5040

aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt   5100

cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcatg gtttaataag   5160

aagagaaaag agttcttttg ttatggctga agtaatagag aaatgagctc gagcgtgtcc   5220

tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt   5280

gtgcgtcatc ccttacgtca gtggagatgt cacatcaatc cacttgcttt gaagacgtgg   5340

ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac   5400

tgtcggcaga ggcatcttga atgatagcct ttcctttatc gcaatgatgg catttgtagg   5460

agccaccttc cttttctact gtcctttcga tgaagtgaca gatagctggg caatggaatc   5520

cgaggaggtt tcccgaaatt atcctttgtt gaaaagtctc aatagccctt tggtcttctg   5580

agactgtatc tttgacattt ttggagtaga ccagagtgtc gtgctccacc atgttgacga   5640

agattttctt cttgtcattg agtcgtaaaa gactctgtat gaactgttcg ccagtcttca   5700

cggcgagttc tgttagatcc tcgatttgaa tcttagactc catgcatggc cttagattca   5760

gtaggaacta cctttttaga gactccaatc tctattactt gccttggttt atgaagcaag   5820

ccttgaatcg tccatactgg aatagtactt ctgatcttga gaaatatgtc tttctctgtg   5880

ttcttgatgc aattagtcct gaatcttttg actgcatctt taaccttctt gggaaggtat   5940

ttgatctcct ggagattgtt actcgggtag atcgtcttga tgagacctgc tgcgtaggcc   6000

tctctaacca tctgtgggtc agcattcttt ctgaaattga agaggctaac cttctcatta   6060

tcagtggtga acatagtgtc gtcaccttca ccttcgaact tccttcctag atcgtaaaga   6120

tagaggaaat cgtccattgt aatctccggg gcaaaggaga tctcttttgg ggctggatca   6180

ctgctgggcc ttttggttcc tagcgtgagc cagtgggctt tttgctttgg tgggcttgtt   6240

agggccttag caaagctctt gggcttgagt tgagcttctc ctttggggat gaagttcaac   6300

ctgtctgttt gctgacttgt tgtgtacgcg tcagctgctg ctcttgcctc tgtaatagtg   6360

gcaaatttct tgtgtgcaac tccgggaacg ccgtttgttg ccgcctttgt acaaccccag   6420

tcatcgtata taccggcatg tggaccgtta tacacaacgt agtagttgat atgagggtgt   6480

tgaatacccg attctgctct gagaggagca actgtgctgt taagctcaga tttttgtggg   6540

attggaattg gatcgatctc gatcccgcga aattaatacg actcactata gggagaccac   6600

aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata cccatggaaa   6660

agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct   6720

ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag   6780

ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg   6840

tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat   6900

tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc   6960

tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggctatg gatgcgatcg   7020

ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc   7080

aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc   7140

aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc   7200

tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca   7260

atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg   7320

gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg   7380

agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc   7440

gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt   7500

tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga   7560

ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag   7620

aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagt   7680

gaggtacagc ttggatcgat ccggctgcta acaaagcccg aaaggaagct gagttggctg   7740

ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg   7800

gttttttgct gaaaggagga actatatccg gatgatcggg cgcgccgtcg acggatccgt   7860

acgagatccg gccggccaga tcctgcagcc cgggggatcc gcccacgtac gcaaaggcaa   7920

agatttaaac tcgaaaacat tacaaaagtc tcaaaacaga ggcaaggcca tgcacaaagc   7980

acactctaag tgcttccatt gcctactaag tagggtacgt acacgatcac cattcaccag   8040

tgatgatctt tattaatata caacacactc agagacagct tatgttatag ctagctagca   8100

taaactatca catcatgtgt tagtacgaca agtgacaaca ttgcttttaa cttcgcggcc   8160

ttggatcctc tagaccggat ataatgagcc gtaaacaaag atgattaagt agtaattaat   8220

acgtactagt aaaagtggca aaagataacg agaaagaacc aatttctttg cattcggcct   8280

tagcggaagg catatataag ctttgattat tttatttagt gtaatgattt cgtacaacca   8340

aagcatttat ttagtactct cacacttgtg tcgcggccgc ttactgagcc ttggcaccgg   8400

gctgcttctc ggccattcga gcgaactggg acaggtatcg gagcaggatg acgagacctt   8460

catggggcag agggtttcgg taggggaggt tgtgcttctg gcacagctgt tccacctggt   8520

aggaaacggc agtgaggttg tgtcgaggca gggtgggcca gagatggtgc tcgatctggt   8580

agttcaggcc tccaaagaac cagtcagtaa tgatgcctcg tcgaatgttc atggtctcat   8640

ggatctgacc cacagagaag ccatgtccgt cccagacgga atcaccgatc ttctccagag   8700

ggtagtggtt catgaagacc acgatggcaa ttccgaagcc accgacgagc tcggaaacaa   8760

agaacaccag catcgaggtc aggatggagg gcataaagaa gaggtggaac agggtcttga   8820

gagtccagtg cagagcgagt ccaatggcct ctttcttgta ctgagatcgg tagaactggt   8880

tgtctcggtc cttgagggat cgaacggtca gcacagactg gaaacaccag atgaatcgca   8940

ggagaataca gatgaccagg aaatagtact gttggaactg aatgagcttt cgggagatgg   9000

gagaagctcg agtgacatcg tcctcggacc aggcgagcag aggcaggtta tcaatgtcgg   9060

gatcgtgacc ctgaacgttg gtagcagaat gatgggcgtt gtgtctgtcc ttccaccagg   9120

tcacggagaa gccctggagt ccgttgccaa agaccagacc caggacgtta ttccagtttc   9180

ggttcttgaa ggtctggtgg tggcagatgt catgagacag ccatcccatt tgctggtagt   9240

gcataccgag cacgagagca ccaatgaagt acaggtggta ctggaccagc atgaagaagg   9300

caagcacgcc aagacccagg gtggtcaaga tcttgtacga gtaccagagg ggagaggcgt   9360

caaacatgcc agtggcgatc agctcttctc ggagctttcg gaaatcctcc tgagcttcgt   9420

tgacggcagc ctggggaggc agctcggaag cctggttgat cttgggcatt cgcttgagct   9480

tgtcgaaggc ttcctgagag tgcataacca tgaaggcgtc agtagcatct cgtccctggt   9540

agttctcaat gatttcagct ccaccagggt ggaagttcac ccaagcggag acgtcgtaca   9600

cctttccgtc gatgacgagg ggcagagcct gtcgagaagc cttcaccatg gtgcggccgc   9660

ttggggggct atggaagact ttcttagtta gttgtgtgaa taagcaatgt tgggagaatc   9720

gggactactt ataggatagg aataaaacag aaaagtatta agtgctaatg aaatatttag   9780

actgataatt aaaatcttca cgtatgtcca cttgatataa aaacgtcagg aataaaggaa   9840

gtacagtaga atttaaaggt actcttttta tatatacccg tgttctcttt ttggctagct   9900

agttgcataa aaaataatct atatttttat cattatttta aatatcttat gagatggtaa   9960

atatttatca taattttttt tactattatt tattatttgt gtgtgtaata catatagaag  10020

ttaattacaa attttattta ctttttcatt attttgatat gattcaccat taatttagtg  10080

ttattattta taatagttca ttttaatctt tttgtatata ttatgcgtgc agtacttttt  10140

tcctacatat aactactatt acattttatt tatataatat ttttattaat gaattttcgt  10200

gataatatgt aatattgttc attattattt cagatttttt aaaaatattt gtgttattat  10260

ttatgaaata tgtaattttt ttagtatttg attttatgat gataaagtgt tctaaattca  10320

aaagaagggg gaaagcgtaa acattaaaaa acgtcatcaa acaaaaacaa aatcttgtta  10380

ataaagataa aactgtttgt tttgatcact gttatttcgt aatataaaaa cattatttat  10440

atttatattg ttgacaacca aatttgccta tcaaatctaa ccaatataat gcatgcgtgg  10500

caggtaatgt actaccatga acttaagtca tgacataata aaccgtgaat ctgaccaatg  10560

catgtaccta nctaaattgt atttgtgaca cgaagcaaat gattcaattc acaatggaga  10620

tgggaaacaa ataatgaaga acccagaact aagaaagctt ttctgaaaaa taaaataaag  10680

gcaatgtcaa aagtatactg catcatcagt ccagaaagca catgatattt ttttatcagt  10740

atcaatgcag ctagttttat tttacaatat cgatatagct agtttaaata tattgcagct  10800

agatttataa atatttgtgt tattatttat catttgtgta atcctgtttt tagtatttta  10860

gtttatatat gatgataatg tattccaaat ttaaaagaag ggaaataaat ttaaacaaga  10920

aaaaaagtca tcaaacaaaa aacaaatgaa agggtggaaa gatgttacca tgtaatgtga  10980

atgttacagt atttctttta ttatagagtt aacaaattaa ctaatatgat tttgttaata  11040

atgataaaat atttttttta ttattatttc ataatataaa aatagtttac ttaatataaa  11100

aaaaattcta tcgttcacaa caaagttggc cacctaattt aaccatgcat gtacccatgg  11160

accatattag gtaaccatca aacctgatga agagataaag agatgaagac ttaagtcata  11220

acacaaaacc ataaaaaaca aaaatacaat caaccgtcaa tctgaccaat gcatgaaaaa  11280

gctgcaatag tgagtggcga cacaaagcac atgattttct tacaacggag ataaaaccaa  11340

aaaaatattt catgaacaac ctagaacaaa taaagctttt atataataaa tatataaata  11400

aataaaggct atggaataat atacttcaat atatttggat taaataaatt gttggcgggg  11460

ttgatatatt tatacacacc taaagtcact tcaatctcat tttcacttaa cttttatttt  11520

ttttttcttt ttatttatca taaagagaat attgataata tactttttaa catattttta  11580

tgacattttt tattggtgaa aacttattaa aaatcataaa ttttgtaagt tagatttatt  11640

taaagagttc ctcttcttat tttaaatttt ttaataaatt tttaaataac taaaatttgt  11700

gttaaaaatg ttaaaaaatg tgttattaac ccttctcttc gaggacgtac gtcgagtcga  11760

cctgca                                                             11766


<210>  187
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer oEugEL1-1

<400>  187
agcggccgca ccatggaggt ggtgaatgaa                                      30


<210>  188
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer oEugEL1-2

<400>  188
tgcggccgct cactgaatct ttttggctcc                                      30


<210>  189
<211>  4311
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKR906

<400>  189
agcggccgca ccatggaggt ggtgaatgaa atagtctcaa ttgggcagga agttttaccc     60

aaagttgatt atgcccaact ctggagtgat gccagtcact gtgaggtgct ttacttgtcc    120

atcgcatttg tcatcttgaa gttcactctt ggcccccttg gtccaaaagg tcagtctcgt    180

atgaagtttg ttttcaccaa ttacaacctt ctcatgtcca tttattcgtt gggatcattc    240

ctctcaatgg catatgccat gtacaccatc ggtgttatgt ctgacaactg cgagaaggct    300

tttgacaaca acgtcttcag gatcaccacg cagttgttct atttgagcaa gttcctggag    360

tatattgact ccttctattt gccactgatg ggcaagcctc tgacctggtt gcaattcttc    420

catcatttgg gggcaccgat ggatatgtgg ctgttctata attaccgaaa tgaagctgtt    480

tggatttttg tgctgttgaa tggtttcatc cactggatca tgtacggtta ttattggacc    540

agattgatca agctgaagtt ccccatgcca aaatccctga ttacatcaat gcagatcatt    600

caattcaatg ttggtttcta cattgtctgg aagtacagga acattccctg ttatcgccaa    660

gatgggatga ggatgtttgg ctggttcttc aattactttt atgttggcac agtcttgtgt    720

ttgttcttga atttctatgt gcaaacgtat atcgtcagga agcacaaggg agccaaaaag    780

attcagtgag cggccgcacc tgaattccag cacactggcg gccgttacta gtggatccga    840

gctcggtacc aagcttgatg catagcttga gtattctaac gcgtcaccta aatagcttgg    900

cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca    960

acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca   1020

cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc   1080

attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt   1140

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact   1200

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag   1260

caaaaggcca gcaaaagccc aggaaccgta aaaaggccgc gttgctggcg tttttccata   1320

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc   1380

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg   1440

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc   1500

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg   1560

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc   1620

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga   1680

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg   1740

gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa   1800

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg   1860

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt   1920

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat   1980

tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt agcacgtgtc   2040

agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact   2100

cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt   2160

tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc   2220

aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt   2280

cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt   2340

cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg   2400

tcaacttggc catggtggcc ctcctcacgt gctattattg aagcatttat cagggttatt   2460

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc   2520

gcacatttcc ccgaaaagtg ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag   2580

gagaaaatac cgcatcagga aattgtaagc gttaataatt cagaagaact cgtcaagaag   2640

gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg   2700

gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg   2760

atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc   2820

caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg   2880

catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc   2940

cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg   3000

tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc   3060

atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc   3120

cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc   3180

tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc   3240

attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag   3300

ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag   3360

cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa   3420

cgatcctcat cctgtctctt gatcagagct tgatcccctg cgccatcaga tccttggcgg   3480

cgagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc   3540

tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag   3600

cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca   3660

gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgaaaag   3720

gatctaggtg aagatccttt ttgataatct catgcctgac atttatattc cccagaacat   3780

caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc   3840

gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc   3900

gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc   3960

cagggggatc accatccgtc gccccggcgt gtcaataata tcactctgta catccacaaa   4020

cagacgataa cggctctctc ttttataggt gtaaacctta aactgccgta cgtataggct   4080

gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa   4140

agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg   4200

ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggccctct   4260

agatgcatgc tcgagcggcc gccagtgtga tggatatctg cagaattcag g            4311


<210>  190
<211>  7085
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKR72

<400>  190
gtacggatcc gtcgacggcg cgcccgatca tccggatata gttcctcctt tcagcaaaaa     60

acccctcaag acccgtttag aggccccaag gggttatgct agttattgct cagcggtggc    120

agcagccaac tcagcttcct ttcgggcttt gttagcagcc ggatcgatcc aagctgtacc    180

tcactattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac    240

ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac    300

agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc    360

gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc    420

ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg    480

ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc    540

cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac    600

attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca    660

aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt    720

ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta    780

ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc    840

agcgatcgca tccatagcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg    900

caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct    960

gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata   1020

aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg   1080

ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc   1140

ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg   1200

cttttccatg ggtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg   1260

ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatcga tccaattcca   1320

atcccacaaa aatctgagct taacagcaca gttgctcctc tcagagcaga atcgggtatt   1380

caacaccctc atatcaacta ctacgttgtg tataacggtc cacatgccgg tatatacgat   1440

gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg gagttgcaca caagaaattt   1500

gccactatta cagaggcaag agcagcagct gacgcgtaca caacaagtca gcaaacagac   1560

aggttgaact tcatccccaa aggagaagct caactcaagc ccaagagctt tgctaaggcc   1620

ctaacaagcc caccaaagca aaaagcccac tggctcacgc taggaaccaa aaggcccagc   1680

agtgatccag ccccaaaaga gatctccttt gccccggaga ttacaatgga cgatttcctc   1740

tatctttacg atctaggaag gaagttcgaa ggtgaaggtg acgacactat gttcaccact   1800

gataatgaga aggttagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga   1860

gaggcctacg cagcaggtct catcaagacg atctacccga gtaacaatct ccaggagatc   1920

aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ttgcatcaag   1980

aacacagaga aagacatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa   2040

ggcttgcttc ataaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttcct   2100

actgaatcta aggccatgca tggagtctaa gattcaaatc gaggatctaa cagaactcgc   2160

cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat   2220

cttcgtcaac atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt   2280

ctcagaagac caaagggcta ttgagacttt tcaacaaagg ataatttcgg gaaacctcct   2340

cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg   2400

ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatg cctctgccga   2460

cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc   2520

aaccacgtct tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc   2580

acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga   2640

gaggacacgc tcgagctcat ttctctatta cttcagccat aacaaaagaa ctcttttctc   2700

ttcttattaa accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat   2760

cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc   2820

tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg   2880

tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga   2940

agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca   3000

gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc   3060

ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt   3120

cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga   3180

tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca   3240

ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca   3300

cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga   3360

ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag   3420

gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct   3480

tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca   3540

gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat   3600

cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt   3660

ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg   3720

tccgagggca aaggaatagt gaggtaccta aagaaggagt gcgtcgaagc agatcgttca   3780

aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc   3840

atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta   3900

tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa   3960

aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta   4020

gatcgatgtc gaatcgatca acctgcatta atgaatcggc caacgcgcgg ggagaggcgg   4080

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg   4140

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg   4200

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa   4260

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg   4320

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc   4380

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc   4440

ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc   4500

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg   4560

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc   4620

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga   4680

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc   4740

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac   4800

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg   4860

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc   4920

acgttaaggg attttggtca tgacattaac ctataaaaat aggcgtatca cgaggccctt   4980

tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac   5040

ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc   5100

gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag   5160

agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg   5220

tatcatacac atacgattta ggtgacacta tagaacggcg cgccaagctt gttgaaacat   5280

ccctgaagtg tctcatttta ttttatttat tctttgctga taaaaaaata aaataaaaga   5340

agctaagcac acggtcaacc attgctctac tgctaaaagg gttatgtgta gtgttttact   5400

gcataaatta tgcagcaaac aagacaactc aaattaaaaa atttcctttg cttgtttttt   5460

tgttgtctct gacttgactt tcttgtggaa gttggttgta taaggattgg gacaccattg   5520

tccttcttaa tttaatttta ttctttgctg ataaaaaaaa aaatttcata tagtgttaaa   5580

taataatttg ttaaataacc aaaaagtcaa atatgtttac tctcgtttaa ataattgaga   5640

ttcgtccagc aaggctaaac gattgtatag atttatgaca atatttactt ttttatagat   5700

aaatgttata ttataataaa tttatataca tatattatat gttatttatt attattttaa   5760

atccttcaat attttatcaa accaactcat aatttttttt ttatctgtaa gaagcaataa   5820

aattaaatag acccacttta aggatgatcc aacctttata cagagtaaga gagttcaaat   5880

agtacccttt catatacata tcaactaaaa tattagaaat atcatggatc aaaccttata   5940

aagacattaa ataagtggat aagtataata tataaatggg tagtatataa tatataaatg   6000

gatacaaact tctctcttta taattgttat gtctccttaa catcctaata taatacataa   6060

gtgggtaata tataatatat aaatggagac aaacttcttc cattataatt gttatgtctt   6120

cttaacactt atgtctcgtt cacaatgcta aggttagaat tgtttagaaa gtcttatagt   6180

acacatttgt ttttgtacta tttgaagcat tccataagcc gtcacgattc agatgattta   6240

taataataag aggaaattta tcatagaaca ataaggtgca tagatagagt gttaatatat   6300

cataacatcc tttgtttatt catagaagaa gtgagatgga gctcagttat tatactgtta   6360

catggtcgga tacaatattc catgctctcc atgagctctt acacctacat gcattttagt   6420

tcatacttgc ggccgcagta tatcttaaat tctttaatac ggtgtactag gatattgaac   6480

tggttcttga tgatgaaaac ctgggccgag attgcagcta tttatagtca taggtcttgt   6540

taacatgcat ggacatttgg ccacggggtg gcatgcagtt tgacgggtgt tgaaataaac   6600

aaaaatgagg tggcggaaga gaatacgagt ttgaggttgg gttagaaaca acaaatgtga   6660

gggctcatga tgggttgagt tggtgaatgt tttgggctgc tcgattgaca cctttgtgag   6720

tacgtgttgt tgtgcatggc ttttggggtc cagttttttt ttcttgacgc ggcgatcctg   6780

atcagctagt ggataagtga tgtccactgt gtgtgattgc gtttttgttt gaattttatg   6840

aacttagaca ttgctatgca aaggatactc tcattgtgtt ttgtcttctt ttgttccttg   6900

gctttttctt atgatccaag agactagtca gtgttgtggc attcgagact accaagatta   6960

attatgatgg gggaaggata agtaactgat tagtacggac tgttaccaaa ttaattaata   7020

agcggcaaat gaagggcatg gatcaaaagc ttggatctcc tgcaggatct ggccggccgg   7080

atctc                                                               7085


<210>  191
<211>  7873
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKR1010

<400>  191
cgcgccgttc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa     60

ttgtagccgc gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct    120

gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg    180

gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg    240

tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc    300

ctatttttat aggttaatgt catgaccaaa atcccttaac gtgagttttc gttccactga    360

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta    420

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa    480

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact    540

gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca    600

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt    660

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg    720

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag    780

cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta    840

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat    900

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg    960

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc   1020

ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac   1080

cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc   1140

gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt   1200

tggccgattc attaatgcag gttgatcgat tcgacatcga tctagtaaca tagatgacac   1260

cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat   1320

gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat   1380

gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc   1440

ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttcgac   1500

gcactccttc tttaggtacc tcactattcc tttgccctcg gacgagtgct ggggcgtcgg   1560

tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg   1620

gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc   1680

tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga   1740

ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc   1800

tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg   1860

gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg   1920

cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact   1980

tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg   2040

acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg   2100

aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc   2160

gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca   2220

gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg   2280

caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg   2340

gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt   2400

acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc   2460

tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca   2520

gacgtcgcgg tgagttcagg ctttttcatg gtttaataag aagagaaaag agttcttttg   2580

ttatggctga agtaatagag aaatgagctc gagcgtgtcc tctccaaatg aaatgaactt   2640

ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca   2700

gtggagatgt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc   2760

acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga   2820

atgatagcct ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttctact   2880

gtcctttcga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgaaatt   2940

atcctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgacattt   3000

ttggagtaga ccagagtgtc gtgctccacc atgttgacga agattttctt cttgtcattg   3060

agtcgtaaaa gactctgtat gaactgttcg ccagtcttca cggcgagttc tgttagatcc   3120

tcgatttgaa tcttagactc catgcatggc cttagattca gtaggaacta cctttttaga   3180

gactccaatc tctattactt gccttggttt atgaagcaag ccttgaatcg tccatactgg   3240

aatagtactt ctgatcttga gaaatatgtc tttctctgtg ttcttgatgc aattagtcct   3300

gaatcttttg actgcatctt taaccttctt gggaaggtat ttgatctcct ggagattgtt   3360

actcgggtag atcgtcttga tgagacctgc tgcgtaggcc tctctaacca tctgtgggtc   3420

agcattcttt ctgaaattga agaggctaac cttctcatta tcagtggtga acatagtgtc   3480

gtcaccttca ccttcgaact tccttcctag atcgtaaaga tagaggaaat cgtccattgt   3540

aatctccggg gcaaaggaga tctcttttgg ggctggatca ctgctgggcc ttttggttcc   3600

tagcgtgagc cagtgggctt tttgctttgg tgggcttgtt agggccttag caaagctctt   3660

gggcttgagt tgagcttctc ctttggggat gaagttcaac ctgtctgttt gctgacttgt   3720

tgtgtacgcg tcagctgctg ctcttgcctc tgtaatagtg gcaaatttct tgtgtgcaac   3780

tccgggaacg ccgtttgttg ccgcctttgt acaaccccag tcatcgtata taccggcatg   3840

tggaccgtta tacacaacgt agtagttgat atgagggtgt tgaatacccg attctgctct   3900

gagaggagca actgtgctgt taagctcaga tttttgtggg attggaattg gatcgatctc   3960

gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat   4020

aattttgttt aactttaaga aggagatata cccatggaaa agcctgaact caccgcgacg   4080

tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg   4140

gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg   4200

gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg   4260

gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat   4320

tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc   4380

gctgttctgc agccggtcgc ggaggctatg gatgcgatcg ctgcggccga tcttagccag   4440

acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat   4500

ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc   4560

gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc   4620

gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc   4680

cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc   4740

gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc   4800

gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt   4860

ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg   4920

cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc   4980

gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga   5040

aaccgacgcc ccagcactcg tccgagggca aaggaatagt gaggtacagc ttggatcgat   5100

ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa   5160

ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga   5220

actatatccg gatgatcggg cgcgccgtcg acggatccgt acgagatccg gccggccaga   5280

tcctgcagga gatccaagct tttgatccat gcccttcatt tgccgcttat taattaattt   5340

ggtaacagtc cgtactaatc agttacttat ccttccccca tcataattaa tcttggtagt   5400

ctcgaatgcc acaacactga ctagtctctt ggatcataag aaaaagccaa ggaacaaaag   5460

aagacaaaac acaatgagag tatcctttgc atagcaatgt ctaagttcat aaaattcaaa   5520

caaaaacgca atcacacaca gtggacatca cttatccact agctgatcag gatcgccgcg   5580

tcaagaaaaa aaaactggac cccaaaagcc atgcacaaca acacgtactc acaaaggtgt   5640

caatcgagca gcccaaaaca ttcaccaact caacccatca tgagccctca catttgttgt   5700

ttctaaccca acctcaaact cgtattctct tccgccacct catttttgtt tatttcaaca   5760

cccgtcaaac tgcatgccac cccgtggcca aatgtccatg catgttaaca agacctatga   5820

ctataaatag ctgcaatctc ggcccaggtt ttcatcatca agaaccagtt caatatccta   5880

gtacaccgta ttaaagaatt taagatatac tgcggccgca ccatggaggt ggtgaatgaa   5940

atagtctcaa ttgggcagga agttttaccc aaagttgatt atgcccaact ctggagtgat   6000

gccagtcact gtgaggtgct ttacttgtcc atcgcatttg tcatcttgaa gttcactctt   6060

ggcccccttg gtccaaaagg tcagtctcgt atgaagtttg ttttcaccaa ttacaacctt   6120

ctcatgtcca tttattcgtt gggatcattc ctctcaatgg catatgccat gtacaccatc   6180

ggtgttatgt ctgacaactg cgagaaggct tttgacaaca acgtcttcag gatcaccacg   6240

cagttgttct atttgagcaa gttcctggag tatattgact ccttctattt gccactgatg   6300

ggcaagcctc tgacctggtt gcaattcttc catcatttgg gggcaccgat ggatatgtgg   6360

ctgttctata attaccgaaa tgaagctgtt tggatttttg tgctgttgaa tggtttcatc   6420

cactggatca tgtacggtta ttattggacc agattgatca agctgaagtt ccccatgcca   6480

aaatccctga ttacatcaat gcagatcatt caattcaatg ttggtttcta cattgtctgg   6540

aagtacagga acattccctg ttatcgccaa gatgggatga ggatgtttgg ctggttcttc   6600

aattactttt atgttggcac agtcttgtgt ttgttcttga atttctatgt gcaaacgtat   6660

atcgtcagga agcacaaggg agccaaaaag attcagtgag cggccgcaag tatgaactaa   6720

aatgcatgta ggtgtaagag ctcatggaga gcatggaata ttgtatccga ccatgtaaca   6780

gtataataac tgagctccat ctcacttctt ctatgaataa acaaaggatg ttatgatata   6840

ttaacactct atctatgcac cttattgttc tatgataaat ttcctcttat tattataaat   6900

catctgaatc gtgacggctt atggaatgct tcaaatagta caaaaacaaa tgtgtactat   6960

aagactttct aaacaattct aaccttagca ttgtgaacga gacataagtg ttaagaagac   7020

ataacaatta taatggaaga agtttgtctc catttatata ttatatatta cccacttatg   7080

tattatatta ggatgttaag gagacataac aattataaag agagaagttt gtatccattt   7140

atatattata tactacccat ttatatatta tacttatcca cttatttaat gtctttataa   7200

ggtttgatcc atgatatttc taatatttta gttgatatgt atatgaaagg gtactatttg   7260

aactctctta ctctgtataa aggttggatc atccttaaag tgggtctatt taattttatt   7320

gcttcttaca gataaaaaaa aaattatgag ttggtttgat aaaatattga aggatttaaa   7380

ataataataa ataacatata atatatgtat ataaatttat tataatataa catttatcta   7440

taaaaaagta aatattgtca taaatctata caatcgttta gccttgctgg acgaatctca   7500

attatttaaa cgagagtaaa catatttgac tttttggtta tttaacaaat tattatttaa   7560

cactatatga aatttttttt tttatcagca aagaataaaa ttaaattaag aaggacaatg   7620

gtgtcccaat ccttatacaa ccaacttcca caagaaagtc aagtcagaga caacaaaaaa   7680

acaagcaaag gaaatttttt aatttgagtt gtcttgtttg ctgcataatt tatgcagtaa   7740

aacactacac ataacccttt tagcagtaga gcaatggttg accgtgtgct tagcttcttt   7800

tattttattt ttttatcagc aaagaataaa taaaataaaa tgagacactt cagggatgtt   7860

tcaacaagct tgg                                                      7873


<210>  192
<211>  11752
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pKR1059


<220>
<221>  misc_feature
<222>  (10557)..(10557)
<223>  n is a, c, g, or t

<400>  192
ggagatccaa gcttttgatc catgcccttc atttgccgct tattaattaa tttggtaaca     60

gtccgtacta atcagttact tatccttccc ccatcataat taatcttggt agtctcgaat    120

gccacaacac tgactagtct cttggatcat aagaaaaagc caaggaacaa aagaagacaa    180

aacacaatga gagtatcctt tgcatagcaa tgtctaagtt cataaaattc aaacaaaaac    240

gcaatcacac acagtggaca tcacttatcc actagctgat caggatcgcc gcgtcaagaa    300

aaaaaaactg gaccccaaaa gccatgcaca acaacacgta ctcacaaagg tgtcaatcga    360

gcagcccaaa acattcacca actcaaccca tcatgagccc tcacatttgt tgtttctaac    420

ccaacctcaa actcgtattc tcttccgcca cctcattttt gtttatttca acacccgtca    480

aactgcatgc caccccgtgg ccaaatgtcc atgcatgtta acaagaccta tgactataaa    540

tagctgcaat ctcggcccag gttttcatca tcaagaacca gttcaatatc ctagtacacc    600

gtattaaaga atttaagata tactgcggcc gcaccatgga ggtggtgaat gaaatagtct    660

caattgggca ggaagtttta cccaaagttg attatgccca actctggagt gatgccagtc    720

actgtgaggt gctttacttg tccatcgcat ttgtcatctt gaagttcact cttggccccc    780

ttggtccaaa aggtcagtct cgtatgaagt ttgttttcac caattacaac cttctcatgt    840

ccatttattc gttgggatca ttcctctcaa tggcatatgc catgtacacc atcggtgtta    900

tgtctgacaa ctgcgagaag gcttttgaca acaacgtctt caggatcacc acgcagttgt    960

tctatttgag caagttcctg gagtatattg actccttcta tttgccactg atgggcaagc   1020

ctctgacctg gttgcaattc ttccatcatt tgggggcacc gatggatatg tggctgttct   1080

ataattaccg aaatgaagct gtttggattt ttgtgctgtt gaatggtttc atccactgga   1140

tcatgtacgg ttattattgg accagattga tcaagctgaa gttccccatg ccaaaatccc   1200

tgattacatc aatgcagatc attcaattca atgttggttt ctacattgtc tggaagtaca   1260

ggaacattcc ctgttatcgc caagatggga tgaggatgtt tggctggttc ttcaattact   1320

tttatgttgg cacagtcttg tgtttgttct tgaatttcta tgtgcaaacg tatatcgtca   1380

ggaagcacaa gggagccaaa aagattcagt gagcggccgc aagtatgaac taaaatgcat   1440

gtaggtgtaa gagctcatgg agagcatgga atattgtatc cgaccatgta acagtataat   1500

aactgagctc catctcactt cttctatgaa taaacaaagg atgttatgat atattaacac   1560

tctatctatg caccttattg ttctatgata aatttcctct tattattata aatcatctga   1620

atcgtgacgg cttatggaat gcttcaaata gtacaaaaac aaatgtgtac tataagactt   1680

tctaaacaat tctaacctta gcattgtgaa cgagacataa gtgttaagaa gacataacaa   1740

ttataatgga agaagtttgt ctccatttat atattatata ttacccactt atgtattata   1800

ttaggatgtt aaggagacat aacaattata aagagagaag tttgtatcca tttatatatt   1860

atatactacc catttatata ttatacttat ccacttattt aatgtcttta taaggtttga   1920

tccatgatat ttctaatatt ttagttgata tgtatatgaa agggtactat ttgaactctc   1980

ttactctgta taaaggttgg atcatcctta aagtgggtct atttaatttt attgcttctt   2040

acagataaaa aaaaaattat gagttggttt gataaaatat tgaaggattt aaaataataa   2100

taaataacat ataatatatg tatataaatt tattataata taacatttat ctataaaaaa   2160

gtaaatattg tcataaatct atacaatcgt ttagccttgc tggacgaatc tcaattattt   2220

aaacgagagt aaacatattt gactttttgg ttatttaaca aattattatt taacactata   2280

tgaaattttt ttttttatca gcaaagaata aaattaaatt aagaaggaca atggtgtccc   2340

aatccttata caaccaactt ccacaagaaa gtcaagtcag agacaacaaa aaaacaagca   2400

aaggaaattt tttaatttga gttgtcttgt ttgctgcata atttatgcag taaaacacta   2460

cacataaccc ttttagcagt agagcaatgg ttgaccgtgt gcttagcttc ttttatttta   2520

tttttttatc agcaaagaat aaataaaata aaatgagaca cttcagggat gtttcaacaa   2580

gcttggcgcg ccgttctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg   2640

tattaattgt agccgcgttc taacgacaat atgtccatat ggtgcactct cagtacaatc   2700

tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc   2760

tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc   2820

tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg   2880

atacgcctat ttttataggt taatgtcatg accaaaatcc cttaacgtga gttttcgttc   2940

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg   3000

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg   3060

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca   3120

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg   3180

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg   3240

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga   3300

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac   3360

ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat   3420

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc   3480

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga   3540

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc   3600

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg   3660

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag   3720

cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc   3780

gcgcgttggc cgattcatta atgcaggttg atcgattcga catcgatcta gtaacataga   3840

tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta   3900

ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca   3960

ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc   4020

aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc   4080

ttcgacgcac tccttcttta ggtacctcac tattcctttg ccctcggacg agtgctgggg   4140

cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt   4200

ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat   4260

cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg   4320

tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc   4380

ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag   4440

atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct   4500

gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc   4560

cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac   4620

gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg   4680

catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac   4740

ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc   4800

agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg   4860

gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg   4920

agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag   4980

ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct   5040

tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc   5100

tcgacagacg tcgcggtgag ttcaggcttt ttcatggttt aataagaaga gaaaagagtt   5160

cttttgttat ggctgaagta atagagaaat gagctcgagc gtgtcctctc caaatgaaat   5220

gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt   5280

acgtcagtgg agatgtcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct   5340

ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca   5400

tcttgaatga tagcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt   5460

tctactgtcc tttcgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc   5520

gaaattatcc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg   5580

acatttttgg agtagaccag agtgtcgtgc tccaccatgt tgacgaagat tttcttcttg   5640

tcattgagtc gtaaaagact ctgtatgaac tgttcgccag tcttcacggc gagttctgtt   5700

agatcctcga tttgaatctt agactccatg catggcctta gattcagtag gaactacctt   5760

tttagagact ccaatctcta ttacttgcct tggtttatga agcaagcctt gaatcgtcca   5820

tactggaata gtacttctga tcttgagaaa tatgtctttc tctgtgttct tgatgcaatt   5880

agtcctgaat cttttgactg catctttaac cttcttggga aggtatttga tctcctggag   5940

attgttactc gggtagatcg tcttgatgag acctgctgcg taggcctctc taaccatctg   6000

tgggtcagca ttctttctga aattgaagag gctaaccttc tcattatcag tggtgaacat   6060

agtgtcgtca ccttcacctt cgaacttcct tcctagatcg taaagataga ggaaatcgtc   6120

cattgtaatc tccggggcaa aggagatctc ttttggggct ggatcactgc tgggcctttt   6180

ggttcctagc gtgagccagt gggctttttg ctttggtggg cttgttaggg ccttagcaaa   6240

gctcttgggc ttgagttgag cttctccttt ggggatgaag ttcaacctgt ctgtttgctg   6300

acttgttgtg tacgcgtcag ctgctgctct tgcctctgta atagtggcaa atttcttgtg   6360

tgcaactccg ggaacgccgt ttgttgccgc ctttgtacaa ccccagtcat cgtatatacc   6420

ggcatgtgga ccgttataca caacgtagta gttgatatga gggtgttgaa tacccgattc   6480

tgctctgaga ggagcaactg tgctgttaag ctcagatttt tgtgggattg gaattggatc   6540

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct   6600

agaaataatt ttgtttaact ttaagaagga gatataccca tggaaaagcc tgaactcacc   6660

gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag   6720

ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc   6780

ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt   6840

gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg   6900

acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa   6960

ctgcccgctg ttctgcagcc ggtcgcggag gctatggatg cgatcgctgc ggccgatctt   7020

agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg   7080

cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac   7140

gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac   7200

tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac   7260

aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac   7320

gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc   7380

tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc   7440

cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct   7500

tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca   7560

caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat   7620

agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagtgagg tacagcttgg   7680

atcgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag   7740

caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa   7800

ggaggaacta tatccggatg atcgggcgcg ccgtcgacgg atccgtacga gatccggccg   7860

gccagatcct gcagcccggg ggatccgccc acgtacgcaa aggcaaagat ttaaactcga   7920

aaacattaca aaagtctcaa aacagaggca aggccatgca caaagcacac tctaagtgct   7980

tccattgcct actaagtagg gtacgtacac gatcaccatt caccagtgat gatctttatt   8040

aatatacaac acactcagag acagcttatg ttatagctag ctagcataaa ctatcacatc   8100

atgtgttagt acgacaagtg acaacattgc ttttaacttc gcggccttgg atcctctaga   8160

ccggatataa tgagccgtaa acaaagatga ttaagtagta attaatacgt actagtaaaa   8220

gtggcaaaag ataacgagaa agaaccaatt tctttgcatt cggccttagc ggaaggcata   8280

tataagcttt gattatttta tttagtgtaa tgatttcgta caaccaaagc atttatttag   8340

tactctcaca cttgtgtcgc ggccgcttac tgagccttgg caccgggctg cttctcggcc   8400

attcgagcga actgggacag gtatcggagc aggatgacga gaccttcatg gggcagaggg   8460

tttcggtagg ggaggttgtg cttctggcac agctgttcca cctggtagga aacggcagtg   8520

aggttgtgtc gaggcagggt gggccagaga tggtgctcga tctggtagtt caggcctcca   8580

aagaaccagt cagtaatgat gcctcgtcga atgttcatgg tctcatggat ctgacccaca   8640

gagaagccat gtccgtccca gacggaatca ccgatcttct ccagagggta gtggttcatg   8700

aagaccacga tggcaattcc gaagccaccg acgagctcgg aaacaaagaa caccagcatc   8760

gaggtcagga tggagggcat aaagaagagg tggaacaggg tcttgagagt ccagtgcaga   8820

gcgagtccaa tggcctcttt cttgtactga gatcggtaga actggttgtc tcggtccttg   8880

agggatcgaa cggtcagcac agactggaaa caccagatga atcgcaggag aatacagatg   8940

accaggaaat agtactgttg gaactgaatg agctttcggg agatgggaga agctcgagtg   9000

acatcgtcct cggaccaggc gagcagaggc aggttatcaa tgtcgggatc gtgaccctga   9060

acgttggtag cagaatgatg ggcgttgtgt ctgtccttcc accaggtcac ggagaagccc   9120

tggagtccgt tgccaaagac cagacccagg acgttattcc agtttcggtt cttgaaggtc   9180

tggtggtggc agatgtcatg agacagccat cccatttgct ggtagtgcat accgagcacg   9240

agagcaccaa tgaagtacag gtggtactgg accagcatga agaaggcaag cacgccaaga   9300

cccagggtgg tcaagatctt gtacgagtac cagaggggag aggcgtcaaa catgccagtg   9360

gcgatcagct cttctcggag ctttcggaaa tcctcctgag cttcgttgac ggcagcctgg   9420

ggaggcagct cggaagcctg gttgatcttg ggcattcgct tgagcttgtc gaaggcttcc   9480

tgagagtgca taaccatgaa ggcgtcagta gcatctcgtc cctggtagtt ctcaatgatt   9540

tcagctccac cagggtggaa gttcacccaa gcggagacgt cgtacacctt tccgtcgatg   9600

acgaggggca gagcctgtcg agaagccttc accatggtgc ggccgcttgg ggggctatgg   9660

aagactttct tagttagttg tgtgaataag caatgttggg agaatcggga ctacttatag   9720

gataggaata aaacagaaaa gtattaagtg ctaatgaaat atttagactg ataattaaaa   9780

tcttcacgta tgtccacttg atataaaaac gtcaggaata aaggaagtac agtagaattt   9840

aaaggtactc tttttatata tacccgtgtt ctctttttgg ctagctagtt gcataaaaaa   9900

taatctatat ttttatcatt attttaaata tcttatgaga tggtaaatat ttatcataat   9960

tttttttact attatttatt atttgtgtgt gtaatacata tagaagttaa ttacaaattt  10020

tatttacttt ttcattattt tgatatgatt caccattaat ttagtgttat tatttataat  10080

agttcatttt aatctttttg tatatattat gcgtgcagta cttttttcct acatataact  10140

actattacat tttatttata taatattttt attaatgaat tttcgtgata atatgtaata  10200

ttgttcatta ttatttcaga ttttttaaaa atatttgtgt tattatttat gaaatatgta  10260

atttttttag tatttgattt tatgatgata aagtgttcta aattcaaaag aagggggaaa  10320

gcgtaaacat taaaaaacgt catcaaacaa aaacaaaatc ttgttaataa agataaaact  10380

gtttgttttg atcactgtta tttcgtaata taaaaacatt atttatattt atattgttga  10440

caaccaaatt tgcctatcaa atctaaccaa tataatgcat gcgtggcagg taatgtacta  10500

ccatgaactt aagtcatgac ataataaacc gtgaatctga ccaatgcatg tacctancta  10560

aattgtattt gtgacacgaa gcaaatgatt caattcacaa tggagatggg aaacaaataa  10620

tgaagaaccc agaactaaga aagcttttct gaaaaataaa ataaaggcaa tgtcaaaagt  10680

atactgcatc atcagtccag aaagcacatg atattttttt atcagtatca atgcagctag  10740

ttttatttta caatatcgat atagctagtt taaatatatt gcagctagat ttataaatat  10800

ttgtgttatt atttatcatt tgtgtaatcc tgtttttagt attttagttt atatatgatg  10860

ataatgtatt ccaaatttaa aagaagggaa ataaatttaa acaagaaaaa aagtcatcaa  10920

acaaaaaaca aatgaaaggg tggaaagatg ttaccatgta atgtgaatgt tacagtattt  10980

cttttattat agagttaaca aattaactaa tatgattttg ttaataatga taaaatattt  11040

tttttattat tatttcataa tataaaaata gtttacttaa tataaaaaaa attctatcgt  11100

tcacaacaaa gttggccacc taatttaacc atgcatgtac ccatggacca tattaggtaa  11160

ccatcaaacc tgatgaagag ataaagagat gaagacttaa gtcataacac aaaaccataa  11220

aaaacaaaaa tacaatcaac cgtcaatctg accaatgcat gaaaaagctg caatagtgag  11280

tggcgacaca aagcacatga ttttcttaca acggagataa aaccaaaaaa atatttcatg  11340

aacaacctag aacaaataaa gcttttatat aataaatata taaataaata aaggctatgg  11400

aataatatac ttcaatatat ttggattaaa taaattgttg gcggggttga tatatttata  11460

cacacctaaa gtcacttcaa tctcattttc acttaacttt tatttttttt ttctttttat  11520

ttatcataaa gagaatattg ataatatact ttttaacata tttttatgac attttttatt  11580

ggtgaaaact tattaaaaat cataaatttt gtaagttaga tttatttaaa gagttcctct  11640

tcttatttta aattttttaa taaattttta aataactaaa atttgtgtta aaaatgttaa  11700

aaaatgtgtt attaaccctt ctcttcgagg acgtacgtcg agtcgacctg ca          11752


<210>  193
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M13F Universal primer

<400>  193
tgtaaaacga cggccagt                                                   18


<210>  194
<211>  757
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (677)..(677)
<223>  n is a, c, g, or t

<400>  194
ttttttttcg aacacttaat ggaggtggtg aatgaaatag tctcaattgg gcaggaagtt     60

ttacccaaag ttgattatgc ccaactctgg agtgatgcca gtcactgtga ggtgctttac    120

ttgtccatcg catttgtcat cttgaagttc actcttggcc cccttggtcc aaaaggtcag    180

tctcgtatga agtttgtttt caccaattac aaccttctca tgtccattta ttcgttggga    240

tcattcctct caatggcata tgccatgtac accatcggtg ttatgtctga caactgcgag    300

aaggcttttg acaacaacgt cttcaggatc accacgcagt tgttctattt gagcaagttc    360

ctggagtata ttgactcctt ctatttgcca ctgatgggca agcctctgac ctggttgcaa    420

ttcttccatc atttgggggc accgatggat atgtggctgt tctataatta ccgaaatgaa    480

gctgtttgga tttttgtgct gttgaatggt ttcatccact ggatcatgta cggttattat    540

tggaccagat tgatcaagct gaagttcccc atgccaaaat ccctgattac atcaatgcag    600

atcattcaat tcaatgttgg tttctacatt gtctggaagt acaggaacat tccctgttat    660

cgccaagatg ggatgangat gtttggctgg ttcttcaatt acttttatgt tggcacagtc    720

ttgtgtttgt tcttgaattt ctatgtgcaa acgtata                             757


<210>  195
<211>  774
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (69)..(69)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (112)..(112)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (218)..(220)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (707)..(709)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (711)..(711)
<223>  n is a, c, g, or t

<400>  195
tcaggatcac cacgcagttg ttctatttga gcangttcct ggagtatatt gactccttct     60

atttgccant gatgggcaag cntctgacct ggttgcaatt cttccatcat tngggggcac    120

cgatggatat gtggctgttc tataattacc gaaatgaagc tgtttggatt tttgtgctgt    180

tgaatggttt catccactgg atcatgtacg gttattannn gaccagattg atcaagctga    240

agttccccat gccaaaatcc ctgattacat caatgcagat cattcaattc aatgttggtt    300

tctacattgt ctggaagtac aggaacattc cctgttatcg ccaagatggg atgaggatgt    360

ttggctggtt cttcaattac ttttatgttg gcacagtctt gtgtttgttc ttgaatttct    420

atgtgcaaac gtatatcgtc aggaagcaca agggagccaa aaagattcag tgatatttcc    480

tcctctgcgg tggcctcttt tgacctcccc ttgacaccta taatgtggag gtgtcgggct    540

ctctccgtct caccagcact tgactctgca ggtgctcact tttatttttt acccatcttt    600

gcttgttgac cattcacctc tcccacttcc acatagtcca ttctaactgt tgcagactgc    660

ggtccatttt ttccagagct cccaatgacc atacgcgaca ccttgtnnnc ncccagccca    720

ttgtgcacaa ttcatagtgg catcgttttg ccttgatacg tgtgcatcca gcgg          774


<210>  196
<211>  1201
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1134)..(1136)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1138)..(1138)
<223>  n is a, c, g, or t

<400>  196
gacatggcaa ctatgatttt attttgactg atagtgacct gttcgttgca acaaattgat     60

gagcaatgct tttttataat gccaactttg tacaaaaaag ttggattttt tttcgaacac    120

ttaatggagg tggtgaatga aatagtctca attgggcagg aagttttacc caaagttgat    180

tatgcccaac tctggagtga tgccagtcac tgtgaggtgc tttacttgtc catcgcattt    240

gtcatcttga agttcactct tggccccctt ggtccaaaag gtcagtctcg tatgaagttt    300

gttttcacca attacaacct tctcatgtcc atttattcgt tgggatcatt cctctcaatg    360

gcatatgcca tgtacaccat cggtgttatg tctgacaact gcgagaaggc ttttgacaac    420

aacgtcttca ggatcaccac gcagttgttc tatttgagca agttcctgga gtatattgac    480

tccttctatt tgccactgat gggcaagcct ctgacctggt tgcaattctt ccatcatttg    540

ggggcaccga tggatatgtg gctgttctat aattaccgaa atgaagctgt ttggattttt    600

gtgctgttga atggtttcat ccactggatc atgtacggtt attattggac cagattgatc    660

aagctgaagt tccccatgcc aaaatccctg attacatcaa tgcagatcat tcaattcaat    720

gttggtttct acattgtctg gaagtacagg aacattccct gttatcgcca agatgggatg    780

aggatgtttg gctggttctt caattacttt tatgttggca cagtcttgtg tttgttcttg    840

aatttctatg tgcaaacgta tatcgtcagg aagcacaagg gagccaaaaa gattcagtga    900

tatttcctcc tctgcggtgg cctcttttga cctccccttg acacctataa tgtggaggtg    960

tcgggctctc tccgtctcac cagcacttga ctctgcaggt gctcactttt attttttacc   1020

catctttgct tgttgaccat tcacctctcc cacttccaca tagtccattc taactgttgc   1080

agactgcggt ccattttttc cagagctccc aatgaccata cgcgacacct tgtnnncncc   1140

cagcccattg tgcacaattc atagtggcat cgttttgcct tgatacgtgt gcatccagcg   1200

g                                                                   1201


<210>  197
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S consensus, optionally comprising M1, M2, M3, M6, M8,
       M12, M14, M15, M16, M18, M19, M21, M22, M26, M38, M39, M40, M41, 
       M45, M46, M49, M50, M51, M53, M54, M58, M63, M68, M69 and M70 
       mutation sites


<220>
<221>  misc_feature
<222>  (2)..(1270)
<223>  mutant delta-8 desaturase CDS

<400>  197
catggtgaag nnnnnncgac aggctnnncc cctcnnnatc gacggannnn nntacnnnnn     60

ntccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact accagggacg    120

agatgctact gacgccttca tggttatgca ctctcaggaa nnnnnngaca agctcnnncg    180

aatgcccaag nnnnnnnnnn nntccnnnnn ncctccccag gctnnnnnna acgaagctca    240

ggaggatttc cgaaagctcc gagaagagct gatcgccact ggcatgtttg acgcctctcc    300

cctctggtac tcgtacaaga tcnnnaccac cctgggtctt ggcgtgcttn nnnnnttcnn    360

nnnnnnncag tacnnnnnnn nnttcattgg tgctnnnnnn ctcggtatgc actaccagca    420

aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc gaaactggaa    480

taacnnnnnn ggtctggtct ttggcaacnn nnnncagggc ttctccgtga cctggtggaa    540

ggacagacac aacgcccatc attctgctac caacgttcag ggtcacgatc ccgacattga    600

taacctgcct ctgctcgcct ggtccgagga cgatgtcact cgagcttctc ccatctcccg    660

aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat    720

ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta    780

ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc tcaagnnnnn    840

nttccacctc ttctttatgc cctccatcct gacctcgnnn ctggtgttcn nnnnntccga    900

gctcgtcggt ggcttcggaa ttgccnnnnn ngtcttcatg aaccactacc ctctggagaa    960

gatcggtgat tccgtctggg acggacatgg cttctctgtg ggtcagatcc atgagaccat   1020

gaacattcga cgaggcnnnn nnnnngactg gttctttgga ggcctgaact accagatcga   1080

gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga   1140

acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg aaggtctcgt   1200

catcctgctc cgatacctgn nnnnnttcgc tcgaatggcc gagaagnnnn nnnnnnnnaa   1260

ggctnnntaa gc                                                       1272


<210>  198
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S consensus, optionally comprising M1, M2, M3, M6, M8,
       M12, M14, M15, M16, M18, M19, M21, M22, M26, M38, M39, M40, M41, 
       M45, M46, M49, M50, M51, M53, M54, M58, M63, M68, M69 and M70 
       mutation sites


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X = S (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X = K (synthetic codon-optimized) or S (mutant)

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  X = L (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  X = T (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  X = T (synthetic codon-optimized) or K (mutant)

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  X = T (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  X = D (synthetic codon-optimized) or E (mutant)

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  X = V (synthetic codon-optimized) or I (mutant)

<220>
<221>  MISC_FEATURE
<222>  (54)..(54)
<223>  X = A (synthetic codon-optimized) or G (mutant)

<220>
<221>  MISC_FEATURE
<222>  (55)..(55)
<223>  X = F (synthetic codon-optimized) or Y (mutant)

<220>
<221>  MISC_FEATURE
<222>  (59)..(59)
<223>  X = K (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (64)..(64)
<223>  X = I (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (65)..(65)
<223>  X = N (synthetic codon-optimized) or D (mutant) or Q (mutant)

<220>
<221>  MISC_FEATURE
<222>  (66)..(66)
<223>  X = P (synthetic codon-optimized) or Q (mutant)

<220>
<221>  MISC_FEATURE
<222>  (67)..(67)
<223>  X = S (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (69)..(69)
<223>  X = E (synthetic codon-optimized) or D (mutant)

<220>
<221>  MISC_FEATURE
<222>  (70)..(70)
<223>  X = L (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (75)..(75)
<223>  X = A (synthetic codon-optimized) or G (mutant)

<220>
<221>  MISC_FEATURE
<222>  (76)..(76)
<223>  X = V (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (108)..(108)
<223>  X = S (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (117)..(117)
<223>  X = G (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (118)..(118)
<223>  X = Y (synthetic codon-optimized) or F (mutant)

<220>
<221>  MISC_FEATURE
<222>  (120)..(120)
<223>  X = L (synthetic codon-optimized) or M (mutant)

<220>
<221>  MISC_FEATURE
<222>  (121)..(121)
<223>  X = M (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (122)..(122)
<223>  X = V (synthetic codon-optimized) or S (mutant)

<220>
<221>  MISC_FEATURE
<222>  (125)..(125)
<223>  X = Q (synthetic codon-optimized) or H (mutant)

<220>
<221>  MISC_FEATURE
<222>  (126)..(126)
<223>  X = M (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (127)..(127)
<223>  X = Y (synthetic codon-optimized) or Q (mutant)

<220>
<221>  MISC_FEATURE
<222>  (132)..(132)
<223>  X = V (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (133)..(133)
<223>  X = L (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (162)..(162)
<223>  X = L (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (163)..(163)
<223>  X = V (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (170)..(170)
<223>  X = G (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (171)..(171)
<223>  X = L (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (279)..(279)
<223>  X = T (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (280)..(280)
<223>  X = L (synthetic codon-optimized) or T (mutant)

<220>
<221>  MISC_FEATURE
<222>  (293)..(293)
<223>  X = L (synthetic codon-optimized) or M (mutant)

<220>
<221>  MISC_FEATURE
<222>  (297)..(297)
<223>  X = F (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (298)..(298)
<223>  X = V (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (309)..(309)
<223>  X = I (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (310)..(310)
<223>  X = V (synthetic codon-optimized) or I (mutant)

<220>
<221>  MISC_FEATURE
<222>  (346)..(346)
<223>  X = I (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (347)..(347)
<223>  X = I (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (347)..(347)
<223>  X = I (synthetic codon-optimized) or L (mutant)

<220>
<221>  MISC_FEATURE
<222>  (348)..(348)
<223>  X = T (synthetic codon-optimized) or S (mutant)

<220>
<221>  MISC_FEATURE
<222>  (348)..(348)
<223>  X = T (synthetic codon-optimized) or S (mutant)

<220>
<221>  MISC_FEATURE
<222>  (407)..(407)
<223>  X = A (synthetic codon-optimized) or S (mutant)

<220>
<221>  MISC_FEATURE
<222>  (408)..(408)
<223>  X = V (synthetic codon-optimized) or Q (mutant)

<220>
<221>  MISC_FEATURE
<222>  (416)..(416)
<223>  X = Q (synthetic codon-optimized) or V (mutant)

<220>
<221>  MISC_FEATURE
<222>  (417)..(417)
<223>  X = P (synthetic codon-optimized) or Y (mutant)

<220>
<221>  MISC_FEATURE
<222>  (418)..(418)
<223>  X = A (synthetic codon-optimized) or G (mutant)

<220>
<221>  MISC_FEATURE
<222>  (419)..(419)
<223>  X = G (synthetic codon-optimized) or A (mutant)

<220>
<221>  MISC_FEATURE
<222>  (422)..(422)
<223>  X = L (synthetic codon-optimized) or Q (mutant)

<400>  198

Met Val Lys Xaa Xaa Arg Gln Ala Xaa Pro Leu Xaa Ile Asp Gly Xaa 
1               5                   10                  15      


Xaa Tyr Xaa Xaa Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Xaa Xaa Asp Lys Leu Xaa Arg Met Pro Lys Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Ser Xaa Xaa Pro Pro Gln Ala Xaa Xaa Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Xaa Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Xaa Xaa Phe Xaa Xaa Xaa Gln Tyr Xaa Xaa Xaa Phe 
        115                 120                 125             


Ile Gly Ala Xaa Xaa Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Xaa Xaa Gly Leu Val Phe Gly Asn Xaa Xaa Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Xaa Xaa Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Xaa Leu Val Phe Xaa Xaa Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Xaa Xaa Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Xaa Xaa Xaa Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Xaa Xaa Phe Ala Arg Met Ala Glu Lys Xaa 
                405                 410                 415     


Xaa Xaa Xaa Lys Ala Xaa 
            420         


